上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
产品信息
Properties | |
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Purification format | Magnetic bead |
Processing format | Manual or automated |
Sample purification (representative examples) | Viral DNA and RNA* from respiratory viruses (enveloped and non-enveloped, dsDNA and ssRNA) |
Sample sources | Saliva, respiratory swab in viral transport media (VTM)** |
Sample input volume | Up to 200 μl** |
Carrier supplier | Poly A carrier RNA*** |
Binding capacity | Up to 3 μg |
Elution volume | 33–100 μl |
Tested automation platforms | KingFisher Flex; Agilent Bravo and MGISP liquid handlers |
Compatible downstream applications | qPCR, RT-qPCR, ddPCR, library prep for NGS |
* Viral DNA and RNA are purified in parallel. Preparation of DNA-free RNA or RNA-free DNA requires further treatment with the appropriate nuclease (not supplied).
** The sample input volume may be scalable to accommodate larger sample volumes. Further workflow optimization may be required.
*** Use of carrier RNA is recommended for recovery of low amounts of viral nucleic acid. Carrier RNA should be omitted if the downstream application utilizes poly(A) RNA enrichment; however, viral nucleic acid recovery may be reduced.
Silica-coated Monarch Mag Beads M1 are utilized for highly sensitive nucleic acid capture from samples containing few target molecules. Silanol groups on the bead shell provide an optimal binding surface for nucleic acids, and the uniform, submicron bead size offers a high surface area with abundant binding sites. Beads are supplied as a monodispersed suspension. The superparamagnetic properties of the bead core result in a fast magnetic response, contributing to ease of handling during use and compatibility with automation
An optimized viral nucleic acid extraction procedure employs a sample lysis step followed by a simple bind-wash-elute process. Samples are treated with Proteinase K and then mixed with a lysis buffer/bead mixture containing lysis buffer, carrier RNA, isopropanol, and magnetic beads, which promote binding of viral nucleic acid onto the silica-coated beads. Carrier RNA is utilized as a co-precipitant in the workflow to enhance the recovery of low amounts of viral nucleic acid. After binding of viral DNA and RNA to the magnetic beads, the beads are washed to remove contaminants, and nucleic acid is eluted in nuclease-free water. Purified viral nucleic acid is suitable for downstream applications, including qPCR/RT-qPCR, ddPCR, and library prep for Next Generation Sequencing (NGS).
- 产品类别:
- Nucleic Acid Purification Products
- 应用:
- Nucleic Acid Purification
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试剂盒组成
本产品提供以下试剂或组分:
NEB # 名称 组分货号 储存温度 数量 浓度 -
T4010S 25 Monarch® Mag Beads M1 T4005-21 25 1 x 2.4 ml Not Applicable Monarch® Carrier RNA T4006-21 25 1 x 125 µg Not Applicable Monarch StabiLyse™ DNA/RNA Buffer T2111-1 25 1 x 24 ml Not Applicable Monarch® Buffer BX T2041-1 25 1 x 19 ml Not Applicable Nuclease-free Water B1500-2 25 1 x 25 ml Not Applicable Proteinase K, Molecular Biology Grade P8200AAVIAL -20 1 x 0.55 ml 20 mg/ml
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T4010L 25 Monarch® Mag Beads M1 T4005-1 25 1 x 14 ml Not Applicable Monarch® Carrier RNA T4006-1 25 1 x 750 µg Not Applicable Monarch StabiLyse™ DNA/RNA Buffer T2111-3 25 1 x 145 ml Not Applicable Monarch® Buffer BX T2041-3 25 1 x 122 ml Not Applicable Nuclease-free Water B1500-3 25 1 x 122 ml Not Applicable Proteinase K, Molecular Biology Grade P8200SVIAL -20 3 x 1 ml 20 mg/ml Proteinase K, Molecular Biology Grade P8200AAVIAL -20 1 x 0.55 ml 20 mg/ml
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T4010X 25 Monarch® Mag Viral DNA/RNA Extraction Kit T4010L 25 Monarch® Mag Beads M1 T4005-1 25 3 x 14 ml Not Applicable Monarch® Carrier RNA T4006-1 25 3 x 750 µg Not Applicable Monarch StabiLyse™ DNA/RNA Buffer T2111-3 25 3 x 145 ml Not Applicable Monarch® Buffer BX T2041-3 25 3 x 122 ml Not Applicable Nuclease-free Water B1500-3 25 3 x 122 ml Not Applicable Proteinase K, Molecular Biology Grade P8200SVIAL -20 9 x 1 ml 20 mg/ml Proteinase K, Molecular Biology Grade P8200AAVIAL -20 3 x 0.55 ml 20 mg/ml
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相关产品
相关产品
- Monarch® 总 RNA 小量提取试剂盒
- Monarch® 基因组 DNA 提取试剂盒
- Luna® SARS-CoV-2 多重 RT-qPCR 检测试剂盒
操作说明、说明书 & 用法
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操作说明
- Reagent Preparation Guidance
- Protocol for Manual Isolation of Viral DNA/RNA in Microfuge Tubes (1.5 or 2.0 ml)
- Protocol Guidance for Automated Isolation of Viral DNA/RNA in Deep Well Plates
- Protocol for KingFisher Flex Automated Isolation of Viral DNA/RNA
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说明书
产品说明书包含产品使用的详细信息、产品配方和质控分析。- manualT4010
FAQs & 问题解决指南
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FAQs
- The enzymes in the kit were not stored at -20°C right away. Will they still work?
- How do I prepare Viral DNA/RNA Wash Buffer?
- Does the kit include Nuclease-Free Water to prepare all wash buffers?
- Where can I find automation scripts?
- What is the sample input volume range?
- What sample types are compatible with T4010?
- What downstream applications are compatible with T4010?
- What is the purpose of Carrier RNA, and is it necessary to include in the extraction?