上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
E. coli K12 ER2738
A suspension of E. coli strain ER2738 that has been grown in LB medium and stored as a 50% glycerol stock.
Storage Temperature: -70°C

上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
A suspension of E. coli strain ER2738 that has been grown in LB medium and stored as a 50% glycerol stock.
Storage Temperature: -70°C
产品信息
F´proA+B+ lacIq Δ(lacZ)M15 zzf::Tn10(TetR)/ fhuA2 glnV Δ(lac-proAB) thi-1 Δ(hsdS-mcrB)5
LB
37°C
质控、安全 & 法规
上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
我们很高兴宣布,我们即将完成把所有反应缓冲液转换为无 BSA(牛血清白蛋白)缓冲液。从 2021 年 4 月开始,NEB 将含有 BSA 的反应缓冲液转换为含有重组白蛋白(rAlbumin)的缓冲液,用于限制性内切酶和某些 DNA 修饰酶。网站上的所有信息均已根据此变化更新。如需了解更多信息,请访问 www.neb.cn/BSA-free。
产品信息
本酶提供高保真版 MfeI-HF®(NEB #R3589)
高保真(HF)限制性内切酶在 rCutSmart 缓冲液中具有 100% 活性;统一缓冲液意味着更加直接、简化的样品处理过程。HF 内切酶还会显著降低星号活性。所有 HF 内切酶均符合省时酶(Time-Saver)标准,可在 5-15 分钟内酶切底物 DNA,也可实现过夜酶切,而且不会造成 DNA 降解。HF 限制性内切酶在改造时便将性能作为重要指标,可在更宽的条件范围下具有完全活性,最大限度地减少非特异性酶切产物,同时为实验设计提供灵活性。
大肠杆菌菌株,携带有来自发酵支原体(Mycoplasma fermentans)(N.F. Halden)的 MfeI 基因。
一个单位是指在 50 µl 的总反应体系中,37℃ 下,1 小时内酶切 1 µg λ DNA 所需的酶量。
1X rCutSmart™ 缓冲液
Incubate at 37°C
1X rCutSmart™ 缓冲液
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)
NEBuffer™ r1.1: 75%
NEBuffer™ r2.1: 50%
NEBuffer™ r3.1: 10%
rCutSmart™ Buffer: 100%
10 mM Tris-HCl
50 mM NaCl
1 mM DTT
0.1 mM EDTA
200 µg/ml BSA
50% Glycerol
pH 7.4 @ 25°C
否
dam 甲基化: 不敏感
dcm 甲基化: 不敏感
CpG甲基化: 不敏感
MfeI-HF
MunI
操作说明、说明书 & 用法
工具 & 资源
FAQs & 问题解决指南
引用 & 技术文献
质控、安全 & 法规
产品说明表包含产品的储存温度、有效期和规格,这些文件的命名规则如下:[货号]_[规格]_[版本]
上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
The N-Glycan Sequencing Kit consists of seven well characterized, highly pure, recombinant exoglycosidase enzymes selected to simplify the process of characterizing typical N-linked glycan structures.
Storage Temperature: 4°C
产品信息
The N-Glycan Sequencing Kit consists of seven well characterized, highly pure, recombinant exoglycosidase enzymes selected to simplify the process of characterizing typical N-linked glycan structures. The extreme diversity of glycan structures on a glycoprotein makes elucidation of the profile challenging and often a number of orthogonal approaches are employed to verify the individual structures. The use of sequential or tandem exoglycosidase digestion of oligosaccharides followed by mass spectrometry or capillary electrophoresis analysis provides detailed carbohydrate sequence information and resolves ambiguities. The exoglycosidases are all active in a universal buffer system, GlycoBuffer 1, and can be used in single digests or in combination.
This kit is compatible with N-linked glycans released from a variety of CHO and murine derived antibodies, as well as N-linked glycans released from other glycoproteins. Optimal incubation times and enzyme concentration may vary for more complex glycans. The recommended enzyme quantity is sufficient for digestion of up to 130 pmol of released glycans (equivalent to released glycans from approximately 10 µgs of antibody) labeled with 2AA, 2AB or procainamide. Other commercially available “Instant labels” may require a larger quantity of enzyme or a longer incubation time for complete digestion.
操作说明、说明书 & 用法
FAQs & 问题解决指南
Remove all traces of Acetonitrile before digesting labeled substrate with exoglycosidases for optimal results.
Use PCR tubes to avoid evaporation when incubating reactions overnight.
Include ZnCl2 in reactions with α1-2,3,6 Mannosidase (P0768) for optimal digestion.
Fucosidase O (P0749) requires an 18 hr incubation for complete digest of some instant labelled glycans and complex oligosaccharides with multiple branches.
If using Fucosidase O (P0749) alone, incubation temperature can be increased to 50°C for more efficient digestion.
引用 & 技术文献
质控、安全 & 法规
产品说明表包含产品的储存温度、有效期和规格,这些文件的命名规则如下:[货号]_[规格]_[版本]
上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
This product has been discontinued and is replaced with the High-Fidelity version, NheI-HF (NEB #R3131)
产品信息
High Fidelity (HF) Restriction Enzymes have 100% activity in CutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
An E. coli strain that carries the NheI gene from Neisseria mucosa heidelbergensis (ATCC 25999).
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA (HindIII digest) in 1 hour at 37°C in a total reaction volume of 50 µl.
1X NEBuffer™ 2.1
Incubate at 37°C
1X NEBuffer™ 2.1
50 mM NaCl
10 mM Tris-HCl
10 mM MgCl2
100 µg/ml BSA
(pH 7.9 @ 25°C)
NEBuffer™ 1.1: 100%
NEBuffer™ 2.1: 100%
NEBuffer™ 3.1: 10%
10 mM Tris-HCl
250 mM NaCl
1 mM DTT
0.1 mM EDTA
200 µg/ml BSA
50% Glycerol
0.15% Triton® X-100
pH 7.4 @ 25°C
65°C for 20 min
dam 甲基化: Not Sensitive
dcm 甲基化: Not Sensitive
CpG甲基化: Blocked by Some Combinations of Overlapping
操作说明、说明书 & 用法
工具 & 资源
FAQs & 问题解决指南
引用 & 技术文献
质控、安全 & 法规
产品说明表包含产品的储存温度、有效期和规格,这些文件的命名规则如下:[货号]_[规格]_[版本]