Good Cell-Free DNA Kit alternative products

Product Description

Good Cell-Free DNA Kit alternative products

Cell-Free DNA Blood Collection Tubes (BCTS) alternative products
Boykyo Cell-Free DNA

Boykyo Cell-Free DNA Blood Collection Tubes (BCTS) alternative products

Boykyo Cell-Free DNA Blood Collection Tubes (BCTS) The same product
Japanese website: www.boykyo.com
Chinese website: www.boykyo.com.cn

Contact information

Company: Shanghai Jinpan Biotech Co Ltd
Phone: +86 15221999938
Tel: +86 021-50837765
QQ: 3188791456
Email: sales@jinpanbio.com
Website: www.jinpanbio.com www.jinpanbio.cn

Blood Collection Tube

Boykyo Cell-Free DNA Blood Collection Tubes (BCTS) is a blood collection tube with a formaldehyde-free preservative stabilizing nucleated blood cells. This unique stabilization prevents the release of genomic DNA, allowing isolation of high-quality cell-free DNA which can be further used for a wide range of downstream applications in clinical research studies, drug discovery and diagnostic assay development.

Boykyo Cell-Free DNA Blood Collection Tubes (BCTS) reduces the need for immediate plasma preparation due to its unique stabilization properties. Cell-Free DNA is stable for up to 14 days, while circulating tumor cells are stable for up to 4 days, at room temperature, allowing convenient sample collection, transport and storage.

Boykyo Cell-Free DNA Blood Collection Tubes (BCTS) Catalog Number
Cell-Free DNA Blood Collection Tubes (BCTS) is For Research Use Only. Not for use in diagnostic procedures in the U.S.
6-Tube Pack 691012
100-Tube Box 691013

INSTRUCTIONS FOR USE
INTENDED USE

Boykyo Cell-Free DNA Blood Collection Tubes (BCTS)is a direct draw whole blood collection tube intended for collection, stabilization and transportation of cell-free plasma DNA. This device also stabilizes and preserves cellular genomic DNA present in nucleated blood cells and circulating epithelial cells (tumor cells) found in whole blood. This product has not been cleared by the U.S. Food and Drug Administration for In Vitro Diagnostic use. The product is For Research Use Only. Not for use in diagnostic procedures.

SUMMARY AND PRINCIPLES

Accurate analysis of cf-DNA can be compromised by sample handling, shipping and processing, causing lysis of nucleated blood cells and subsequent release of cellular genomic DNA. Additionally, degradation of cf-DNA due to nuclease activity can be problematic.

The formaldehyde-free preservative reagent contained in Cell-Free DNA Blood Collection Tubes (BCTS) stabilizes nucleated blood cells, preventing the release of cellular genomic DNA, and inhibits nuclease mediated degradation of cf-DNA, contributing to the overall stabilization of cf-DNA3 . Samples collected in Cell-Free DNA Blood Collection Tubes (BCTS) are stable for up to 14 days at temperatures between 6 °C-37 °C, allowing convenient sample collection, transport and storage4.

The formaldehyde-free preservative reagent contained in Cell-Free DNA Blood Collection Tubes (BCTS) stabilizes circulating epithelial cells (tumor cells) in whole blood for up to 4 days at temperatures between 15 °C-30 °C5.

Product Feature
Boykyo Cell-Free DNA Blood Collection Tubes (BCTS)Boykyo Cell-Free DNA Blood Collection Tubes (BCTS) alternative products

Product Specification/Models
Boykyo Cell-Free DNA Blood Collection Tubes (BCTS)Boykyo Cell-Free DNA Blood Collection Tubes (BCTS) alternative products

Application
Boykyo Cell-Free DNA Blood Collection Tubes (BCTS)Boykyo Cell-Free DNA Blood Collection Tubes (BCTS) alternative products

Other Information
Boykyo Cell-Free DNA Blood Collection Tubes (BCTS)Boykyo Cell-Free DNA Blood Collection Tubes (BCTS) alternative products

Payment
TT

Delivery
UPS

Origin
Japan

Minimum Order
1tube

Packaging
100tubes/BOX

Samples
Free

Sample Price
US$7

Remarks
Boykyo Cell-Free DNA Blood Collection Tubes (BCTS)

Contact information

Company: Shanghai Jinpan Biotech Co Ltd
Phone: +86 15221999938
Tel: +86 021-50837765
QQ: 3188791456
Email: sales@jinpanbio.com
Website: www.jinpanbio.com www.jinpanbio.cn

食源性肥胖症饲料    

食源性肥胖症饲料    
肥胖是一种世界范围内的慢性疾病,是导致高血压、糖尿病、冠心病、心肌梗死等多种慢性病发生的主要危险因。

遗传和环境因素对肥胖症的发生起重要作用,膳食则是影响肥胖症的主要的环境因素之一。膳食诱导的肥胖动物模型普遍被用于肥胖发生机理和治疗方法的研究。

我们与世界顶级动物饲料供应商美国RDI合作,为客户提供配方可报告、可复制、可修改的纯化饲料,我们的饲料具有造模时间短、造模效果好、有效易复制等特点。

重点推荐饲料:D12492和D12451。

 推荐产品  货号  规格  生产厂商
60%Kcal High-Fat(DIO) Diets D12492 12.5kg 美国RDI
45%Kcal High-Fat(DIO) Diets D12451 12.5kg 美国RDI
10%Kcal High-Fat(DIO) 7% Sucrose Control Diets match D12492 D12450J 12.5kg 美国RDI
10%Kcal High-Fat(DIO) 17% Sucrose Control Diets match D12451 D12450H 12.5kg 美国RDI
10%Kcal High-Fat(DIO) 35% Sucrose Control Diets D12450B 12.5kg 美国RDI
10%Kcal High-Fat(DIO) No Sucrose Control Diets D12450K 12.5kg 美国RDI
 糖尿病 / 胰岛素抵抗饲料    
精制的碳水化合物如高果糖玉米糖浆被用于许多加工的食品,美国的调查显示,自20世纪70年代开始,这种甜味剂的摄入量明显增加。

过去几十年我们认识到,过多的摄入这种精制的碳水化合物物与人体或动物模型中的体重增加、高脂血症和胰岛素抵抗相关。

在啮齿类动物中,高果糖饮食能够诱导SD大鼠、Wistar 大鼠和仓鼠高甘油三脂血症、胰岛素抵抗和高血压。

SD大鼠和Wistar大鼠是建立蔗糖诱导的胰岛素抵抗和高甘油三脂血症的很好模型。不同于大鼠和仓鼠,小鼠很少被用来建立胰岛素抵抗和高甘油三脂模型。

因为,常用的C57BL/6小鼠给予蔗糖/果糖饮食时,根本不形成胰岛素抵抗或者形成表型非常缓慢。

推荐饲料:D11707,D08040106。

 推荐产品  货号  规格  生产厂商
58%Kcal Diabetes/ Insulin Resistance Diets sucrose D12331 12.5kg 美国RDI
58%Kcal Diabetes/ Insulin Resistance Diets corn starch D12330 12.5kg 美国RDI
11%Kcal Diabetes/ Insulin Resistance Diets sucrose-Control D12329 12.5kg 美国RDI
11%Kcal Diabetes/ Insulin Resistance Diets corn starch-Control D12328 12.5kg 美国RDI
 脂肪肝(NAFLD)饲料    
非酒精性脂肪肝是一系列疾病状态,包括从脂肪变性到非酒精性脂肪性肝炎,其后进展为肝纤维化、肝硬化和肝癌。与人类很多疾病由膳食导致的一样,啮齿类动物的脂肪肝也可由膳食诱导。目前,常用三种膳食类型来诱导脂肪肝,分别为:蛋氨酸和胆碱缺乏(MCD)饲料、胆碱缺乏(CD)饲料和高脂(HFD)饲料。

MCD饲料用于研究肝病已有超过40年的历史,喂食MCD饲料的啮齿类动物将在2-4周内发展出显著脂肪肝,然后迅速演变为肝炎和纤维化。重要的是,与人类和其它方法诱导的NAFLD啮齿类动物模型不一样,喂养MCD饲料的啮齿类动物会减轻体重,且不发生胰岛素抵抗,这与人类典型的NASH患者情况相反,他们不但肥胖而且对胰岛素抵抗。

CD饲料与MCD饲料相比,潜在优势在于不但增加肝脏脂肪水平,而且增加体重,导致血脂异常和胰岛素抵抗。CD饲料所诱发的肝脏脂肪堆积、肝脏损伤和炎症并没有MCD饲料的严重。

HFD可以诱导啮齿类动物模型体重和体脂肪增加并导致胰岛素抵抗。一般来说,HFD喂养与MCD喂养相比,并不产生肝纤维化,且只有轻微脂肪肝。

推荐饲料:A02082002B,D05010401-03。

 推荐产品  货号  规格  生产厂商
Methionine and Choline Deficient Diet A02082002B 12.5kg 美国RDI
Methionine/choline deficient diet with 60 kcal% fat A06071301B 12.5kg 美国RDI
choline deficient diet with 0.1% Methionine A06071302 12.5kg 美国RDI
40 kcal% Fat, 20 kcal% Sucrose, and 2% Cholesterol D09100301 12.5kg 美国RDI
 动脉粥样硬化饲料    
动脉粥样硬化是一种复杂的慢性疾病,其特征为脂质在动脉壁堆积,最终形成版块,从而导致动脉缩小、硬化、以及/或者完全阻塞。

研究表明,含饱和脂肪和胆固醇的食物与循环胆固醇水平升高密切相关。

跟人类一样,西方膳食可诱导某些啮齿类动物(如小鼠、仓鼠和豚鼠)LDL-C升高和动脉粥样硬化。

ApoE敲除或者LDL受体敲除小鼠是诱导动脉粥样硬化的易感小鼠。大鼠很难诱导高胆固醇血症,因为当在膳食中添加胆固醇时,大鼠内源性的胆固醇产生迅速下降。然而,通常采用高脂添加胆固醇的饲料(如D12108C和D12079B)来诱导动脉粥样硬化和胆固醇血症,D12079B比D12108C含有更少的胆固醇,发展成动脉粥样硬化比较缓慢。Veillard等对ApoE敲除小鼠喂养D12108C饲料14周,观察到在第8周时,形成相当严重的病变。

推荐饲料:D12079B,D12108C。

 推荐产品  货号  规格  生产厂商
High fat rodent diet with 1.25% cholesterol D12108C 12.5kg 美国RDI
5% Sodium Cholic Acid,12.5% Cholesterol D12336 12.5kg 美国RDI
 高血压饲料    
人类高血压的发病机制尚未被完全了解,这种血压持续过高的病症是遗传和环境的多因素共同作用的结果。

为了更好的了解其发病机制,促进预防与防治,多种动物模型已经被研发来模仿人类的高血压反应。

高血压研究的首选动物模型是的Dahl或SHR大鼠,小鼠并没被广泛使用。

高钠饲料通常用于研究膳食诱导高血压,因为增加循环钠水平会使细胞释放水分子,从而提升了对血管壁的压力。

推荐饲料:D02011103,D05011703,D10001。

 推荐产品  货号  规格  生产厂商
AIN-76A + 8% Sodium Chloride D05011703 12.5kg 美国RDI
AIN-76A- widely used, balanced,purified rodent diet D10001 12.5kg 美国RDI

LBgard® Blood Tubes

LBgard® Blood Tubes

Robust cfDNA collection for liquid biopsy and NIPT

Boykyo Product Name Pack Size Catalog Number
Cell-Free DNA Kit 6-Tube Box (10 mL) 691012
Cell-Free DNA Kit 100-Tube Box (10 mL) 691013
Cell-Free DNA Kit 1000-Tube Box (10 mL) 691015
Cell-Free DNA Kit 6-Tube Box (2 mL) 691212
Cell-Free DNA Kit 100-Tube Box (2 mL) 691213

Japanese website: www.boykyo.com
Chinese website: www.boykyo.com.cn
English website:www.utopbio.com

Contact information

Company: Shanghai Jinpan Biotech Co Ltd
Phone: +86 15221999938
Tel: +86 021-50837765
QQ: 3188791456
Email: sales@jinpanbio.com
Website: www.jinpanbio.com

Website:www.jinpanbio.cn

  • Preserve cfDNA for 14 days
  • Preserve CTCs and WBCs for 4 days
  • Minimize hemolysis & gDNA release
  • Secure blood samples in plastic tubes
LBgard Blood Tube

Workflow Overview

LBgard Workflow
For Research Use Only. Not for use in diagnostic procedures.

Boykyo Cell-free DNA kit Protocol CELL-FREE DNA KIT 691013

Technical details of Boykyo Cell-free DNA kit 691013

Japanese website: www.boykyo.com
Chinese website: www.boykyo.com.cn

Contact information

Company: Shanghai Jinpan Biotech Co Ltd
Phone: +86 15221999938
Tel: +86 021-50837765
QQ: 3188791456
Email: sales@jinpanbio.com
Website: www.jinpanbio.com www.jinpanbio.cn

Boykyo Cell-free DNA kit Protocol  CELL-FREE DNA KIT 691013

  1. Storage and stability

 

When stored at 2-30 C , the empty Cell-Free DNA Blood Collection tubes is stable through expiration date.

Shelf life of the empty Cell-Free DNA Blood Collection tubes is two years.

When the blood is collected in the tubes, the Cell-Free DNA in the Boykyo Cell-free DNA Blood Collection tubes can be stable for up to 14 days, and circulating tumor cells are stable for up to 7 days, at room temperature between 6-37 C .

 

  1. Temperature

 

The Boykyo Cell-free DNA Blood Collection tubes can be stored at 2-30 C .

The Boykyo Cell-free DNA Blood Collection tubes can be used at room temperature between 6-37 C .

 

  1. Protocol

 

  • Double spin protocols are commonly used to separate blood plasma for Cell‑Free DNA isolation.

300 x g for 20 min followed by 5000 x g for 10 min or 1600 x g for 10 min followed by 16000 x g for10 min

 

  • The single spin protocol can also be used to separate blood plasma for Cell‑Free DNA isolation.

1600 x g for 15 min

 

3.3  Perform DNA extraction from plasma in accordance with assay instructions or standard laboratory protocols. For example, Plasma cfDNA can be purified using the commercially available QIAamp®Circulating Nucleic Acid Kit (QIAGEN®, Santa Clarita, CA). For optimal results, the manufacturer’s recommended protocol was modified slightly by increasing the duration of the proteinase K treatment from 30 min to 1 hour at 60 C . cfDNA samples were stored at ‑80 C  until analysis by PCR.

 

  1. Limitations

 

4.1 Do not use products after their expiration date.

4.2 DO NOT REFRIGERATE OR FREEZE.

4.3 For Research Use Only. Not for use in diagnostic procedures.

4.4 For Professional Use Only.

4.5 The Cell-Free DNA Collection Tube is not intended for pediatric blood draws.

4.6 The Cell-Free DNA Collection Tube is not intended for analytes other than cell-free DNA.

 

 

 

  1. Instructions for use

 

5.1  Blood Collection

General guidelines for specimen collection can be found in Clinical and Laboratory Standards Institute (CLSI) H3-A6, Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture1.

5.1.1  Collect specimen (avoid backflow):

  1. Place the patient’s arm in a downward position.
  2. Hold the tube with the stopper uppermost.
  3. Release tourniquet once blood starts to flow in the tube.
  4. Fill tube in 10 ml
  5. Ensure the tube contents do not touch the stopper or the end of the needle during collection.

5.1.2  Remove tube from adapter.

5.1.3  To avoid hemolysis, immediately mix by gentle inversion 8 to 10 times to ensure adequate mixing of the chemical additives with the blood specimen.

  • Plasma Separation

 

5.2.1   Plasma separation centrifugation protocols included the single spin protocol (1600 x g for 15 min) and two double spin processes(300 x g for 20 min followed by 5000 x g for 10 min or 1600 x g for 10 min followed by 16000 x g for10 min)

 

  • cfDNA Isolation From Plasma

 

5.3.1  Perform DNA extraction from plasma in accordance with assay instructions or standard laboratory protocols. For example, Plasma cfDNA can be purified using the commercially available QIAamp®Circulating Nucleic Acid Kit (QIAGEN®, Santa Clarita, CA). For optimal results, the manufacturer’s recommended protocol was modified slightly by increasing the duration of the proteinase K treatment from 30 min to 1 hour at 60 C . cfDNA samples were stored at ‑80 C  until analysis by PCR.

 

  1. References

Clinical and Laboratory Standards Institute. H3-A6, Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture; Approved Standard-Sixth Edition.