分析物意义：食品添加剂
Megazyme检测试剂盒优点：反应快、试剂稳定
The D-Gluconate/D-Glucono-δ-lactone test kit is suitable for the specific measurement and analysis of D-gluconic acid/D-gluconolactone in foods and beverages.
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
Suitable for manual, auto-analyser and microplate formats.
UV-method for the determination of D-Gluconic Acid and
D-Glucono-δ-lactone in foodstuffs, beverages and other
materials
Principle:
(gluconate kinase)
(1) D-Gluconate + ATP → gluconate-6-phosphate + ADP
(gluconate-6-phosphate dehydrogenase)
(2) Gluconate-6-phosphate + NADP+ → ribulose-5-phosphate +
NADPH + CO2 + H+
(pH 11)
(3) D-Glucono-δ-lactone + H2O → D-gluconate
Kit size: 60 assays (manual) / 600 (microplate)
/ 600 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 6 min
Detection limit: 0.5 mg/L
Application examples:
Wine, meat, processed meat (e.g. additives), fruit juice, dairy products,
pharmaceuticals, paper and other materials (e.g. biological cultures,
samples, etc.)
Method recognition:
Methods based on this principle have been accepted by ISO,
DIN and GOST
Advantages
- All reagents stable for > 2 years after preparation
- Very competitive price (cost per test)
- Very rapid reaction
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
- Extended cofactors stability
- Suitable for manual, microplate and auto-analyser formats