E. coli K12 ER2738 | NEB酶试剂 New England Biolabs

特色

发表于2022年9月24日由Wako和光纯药中国

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

E. coli K12 ER2738

A suspension of E. coli strain ER2738 that has been grown in LB medium and stored as a 50% glycerol stock.

Storage Temperature: -70°C

E. coli K12 ER2738 | NEB酶试剂 New England Biolabs

产品信息

基因型

F´proA⁺B⁺ lacI^q Δ(lacZ)M15 zzf::Tn10(Tet^R)/ fhuA2 glnV Δ(lac-proAB) thi-1 Δ(hsdS-mcrB)5

产品类别:: Strains

特性和用法
抗生素耐药性
- tetracycline
生长基质

LB

培养温度

37°C
注意事项
1. This strain is an fhuA2 version of E. coli NM522 in which the F’ can be selected for using tetracycline.
参考文献
1. Woodcock, D.M. et al. (1989). Nucl.Acids Res.. 17, 3469-3478.
2. Gough, J. and Murray, N. (1983). J. Mol.Biol.. 166, 1-19.

质控、安全 & 法规

质控分析

每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。
CoA
CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]
- E4104S__10007428
- E4104S_v0_10007428
- E4104S_v0_10021632
- E4104S_v0_10036891
- E4104S_v0_10053868
- E4104S_v0_10067105
- E4104S_v0_10080097
- E4104S_v0_10092624
- E4104S_v0_10102635
- E4104S_v0_10098508
- E4104S_v0_10124261
SDS
以下 SDS 文件可以帮助您安全地使用该产品
- E. coli K12 ER2738

Oligo (dT)25 Cellulose Beads | NEB酶试剂 New England Biolabs

发表于2022年9月24日由Wako和光纯药中国

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

Oligo (dT)₂₅ Cellulose Beads

We recommend Oligo d(T)₂₅Magnetic Beads (NEB #S1419S) as an alternative for purification of mRNA containing polyadenylic (poly A) regions. For NGS applications, the NEBNext Poly(A) mRNA Isolation Module (NEB #E7490) is recommended.

Catalog # S1408 was discontinued on March 31, 2020

Used for the isolation of mRNA containing polyadenylic (poly A) regions from purified total RNA
Consists of oligo (dT)₂₅covalently coupled to a cross-linked cellulose bead
Guaranteed for twenty-four months when stored at 4°C in the supplied storage buffer

Storage Temperature: 4°C

精选视频

Oligo (dT)25 Cellulose Beads | NEB酶试剂 New England Biolabs

Avoiding RNase Contamination

产品信息

An affinity matrix used for the isolation of mRNA containing polyadenylic (poly A) regions (1) from purified total RNA. This matrix consists of oligo (dT)₂₅ covalently coupled to a cross-linked cellulose bead. This product is guaranteed for twenty-four months when stored at 4°C in the supplied storage buffer.

产品类别:: Discontinued Products

特性和用法

贮存溶液

0.02% NaN₃
注意事项
1. Refrigerators are usually set at 2-8°C, and the recommended storage temperature for this product is 4°C, so it is safe to store it in the fridge, but it should not be shipped on dry ice.
2. This product is not designed to work with in-vitro RNA.
参考文献
1. Aviv, H. and Leder, P. (1972). Proc. Natl. Acad. Sci. USA. 69, 1408.

引用 & 技术文献

引用文献

产品引用文献查找工具

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质控、安全 & 法规

质控分析

每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。
产品说明与变更通知
产品说明与变更通知

产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]
- S1408S_v1
CoA
CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]
- S1408S_v0_10018421
- S1408S_v1_10040034
- S1408S_v1_10045724
- S1408S_v1_10050896
SDS
以下 SDS 文件可以帮助您安全地使用该产品
- Oligo (dT)₂₅ Cellulose Beads
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MfeI | NEB酶试剂 New England Biolabs

发表于2022年8月26日由Wako和光纯药中国

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

MfeI

我们很高兴宣布，我们即将完成把所有反应缓冲液转换为无 BSA（牛血清白蛋白）缓冲液。从 2021 年 4 月开始，NEB 将含有 BSA 的反应缓冲液转换为含有重组白蛋白（rAlbumin）的缓冲液，用于限制性内切酶和某些 DNA 修饰酶。网站上的所有信息均已根据此变化更新。如需了解更多信息，请访问 www.neb.cn/BSA-free。

Catalog # R0589 was discontinued on December 15, 2021

同裂酶 | 单字母标示 | Pronunciation:

在 rCutSmart^™ 缓冲液中具有 100% 活性（超过 210 种内切酶兼容于此缓冲液），便于进行双酶切
本酶可提供高保真（HF^®）版（NEB #R3589），随附 rCutSmart 缓冲液
限制性内切酶酶切位点：C/AATTG

精选视频

Reduce Star Activity with High-Fidelity Restriction Enzymes

观看其他视频

产品信息

本酶提供高保真版 MfeI-HF®（NEB #R3589）

高保真（HF）限制性内切酶在 rCutSmart 缓冲液中具有 100% 活性；统一缓冲液意味着更加直接、简化的样品处理过程。HF 内切酶还会显著降低星号活性。所有 HF 内切酶均符合省时酶（Time-Saver）标准，可在 5-15 分钟内酶切底物 DNA，也可实现过夜酶切，而且不会造成 DNA 降解。HF 限制性内切酶在改造时便将性能作为重要指标，可在更宽的条件范围下具有完全活性，最大限度地减少非特异性酶切产物，同时为实验设计提供灵活性。

产品来源

大肠杆菌菌株，携带有来自发酵支原体（Mycoplasma fermentans）（N.F. Halden）的 MfeI 基因。

产品类别:: Discontinued Products

特性和用法
单位定义

一个单位是指在 50 µl 的总反应体系中，37℃ 下，1 小时内酶切 1 µg λ DNA 所需的酶量。

反应条件

1X rCutSmart™ 缓冲液
Incubate at 37°C

1X rCutSmart™ 缓冲液
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)

在不同缓冲液中的活性

NEBuffer™ r1.1: 75%
NEBuffer™ r2.1: 50%
NEBuffer™ r3.1: 10%
rCutSmart™ Buffer: 100%

稀释兼容性
- 稀释液 A
贮存溶液

10 mM Tris-HCl
50 mM NaCl
1 mM DTT
0.1 mM EDTA
200 µg/ml BSA
50% Glycerol
pH 7.4 @ 25°C

热失活

否

甲基化敏感性

dam 甲基化: 不敏感
dcm 甲基化: 不敏感
CpG甲基化: 不敏感

同裂酶

MfeI-HF
MunI
相关产品
相关产品
- MfeI-HF®
- Monarch® Plasmid Miniprep Kit
- Monarch® DNA Gel Extraction Kit
- Monarch® PCR & DNA Cleanup Kit (5 μg)
- Nuclease-free Water
单独销售的组分
- rCutSmart™ 缓冲液
注意事项
1. MfeI 是 MunI 的同裂酶。
2. 对 CpG、dcm 或 dam 甲基化不敏感。
3. 延长酶切时间可能产生星号活性。

操作说明、说明书 & 用法

操作说明
1. Optimizing Restriction Endonuclease Reactions
2. Restriction Digest Protocol
3. Double Digest Protocol with Standard Restriction Enzymes
使用指南
- Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
- Activity of Restriction Enzymes in PCR Buffers
- Alteration of Apparent Recognition Specificities Using Methylases
- Cleavage Close to the End of DNA Fragments
- Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
- Double Digests
- Heat Inactivation
- Restriction Endonucleases – Survival in a Reaction
- Restriction Enzyme Diluent Buffer Compatibility
- Restriction Enzyme Tips
- Single Letter Codes
- Star Activity

工具 & 资源

选择指南
- Alphabetized List of Recognition Sequences
- Compatible Cohesive Ends and Generation of New Restriction Sites
- Dam-Dcm and CpG Methylation
- Frequencies of Restriction Sites
- Isoelectric Points (pI) for Restriction Enzymes
- Isoschizomers
- NEB Diluent and Buffer Table
- Recleavable Filled-in 5′ Overhangs
- Time-Saver™ Qualified Enzymes
Web 工具
- DNA Sequences and Maps Tool
- Double Digest Finder
- Enzyme Finder
- NEBcutter™ v3.0
- NEBioCalculator®
- REBASE®

FAQs & 问题解决指南

FAQs
1. Is MfeI inhibited by salt?
2. Does MfeI have trouble cleavin PCR products?
3. What is the molecular weight of MfeI?
4. Does MfeI have single sites in common vectors?
5. What is the activity of MfeI at 25°C?
6. I tested your restriction enzyme on the substrate DNA recommended by NEB, and it appears to be active, however it does not digest my DNA. What could be the reason?
7. Why is my Restriction Enzyme not cutting DNA?
8. Why do I see a DNA smear on an agarose gel after a restriction digest?
9. Why do I see additional DNA bands on my gel after a restriction digest?
10. How many nucleotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?
11. Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?
12. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

引用 & 技术文献

引用文献

产品引用文献查找工具

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质控、安全 & 法规

质控分析

每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。
产品说明与变更通知
产品说明与变更通知

产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]
- R0589S_L_v2
CoA
CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]
- R0589S_L_v1_0081402
- R0589S_L_v1_0081407
- R0589S_L_v1_0081404
- R0589S_L_v1_0081409
- R0589S_L_v1_0081411
- R0589S_L_v1_0071212
- R0589S_L_v1_0081305
- R0589S_L v1_0081311
- R0589S_L_v1_0081503
- R0589S_L_v1_0081509
- R0589S_L_v1_0081510
- R0589S_L_v1_0081602
- R0589S_L_v1_0081604
- R0589S_L_v1_0081607
- R0589S_L_v2_0081611
- R0589S_L_v2_0081703
- R0589S_L_v2_0081705
- R0589S_L_v2_0081710
- R0589S_L_v2_0081712
- R0589S_L_v2_0081803
- R0589L_v2_10012877
- R0589S_v2_10012879
- R0589L_v2_10021707
- R0589L_v2_10028201
- R0589S_v2_10028199
- R0589L_v2_10034798
- R0589S_v2_10034800
- R0589L_v2_10043669
- R0589S_v2_10043671
- R0589L_v2_10052857
- R0589S_v2_10052860
- R0589L_v2_10064278
- R0589S_v2_10064280
- R0589L_v2_10073966
- R0589S_v2_10076924
- R0589L_v2_10079179
- R0589S_v2_10082142
- R0589L_v2_10088520
- R0589L_v2_10092124
- R0589S_v2_10088518
- R0589L_v2_10103873
- R0589S_v2_10104219
- R0589L_v2_10116126
- R0589S_v2_10116130
SDS
以下 SDS 文件可以帮助您安全地使用该产品
- MfeI

N-Glycan Sequencing Kit | NEB酶试剂 New England Biolabs

发表于2022年8月25日由Wako和光纯药中国

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

N-Glycan Sequencing Kit

Recombinant enzymes with no detectable endoglycosidase or other exoglycosidase contaminating activities
Compatible with N-linked glycans released from a variety of CHO and murine derived antibodies, as well as N-linked glycans released from other glycoproteins
Simultaneous digestion with other exoglycosidases
Optimal activity and stability for up to 12 months

Storage Temperature: 4°C

N-Glycan Sequencing Kit | NEB酶试剂 New England Biolabs

产品信息

The N-Glycan Sequencing Kit consists of seven well characterized, highly pure, recombinant exoglycosidase enzymes selected to simplify the process of characterizing typical N-linked glycan structures. The extreme diversity of glycan structures on a glycoprotein makes elucidation of the profile challenging and often a number of orthogonal approaches are employed to verify the individual structures. The use of sequential or tandem exoglycosidase digestion of oligosaccharides followed by mass spectrometry or capillary electrophoresis analysis provides detailed carbohydrate sequence information and resolves ambiguities. The exoglycosidases are all active in a universal buffer system, GlycoBuffer 1, and can be used in single digests or in combination.

This kit is compatible with N-linked glycans released from a variety of CHO and murine derived antibodies, as well as N-linked glycans released from other glycoproteins. Optimal incubation times and enzyme concentration may vary for more complex glycans. The recommended enzyme quantity is sufficient for digestion of up to 130 pmol of released glycans (equivalent to released glycans from approximately 10 µgs of antibody) labeled with 2AA, 2AB or procainamide. Other commercially available “Instant labels” may require a larger quantity of enzyme or a longer incubation time for complete digestion.

产品类别:: Discontinued Products

特性和用法
优势和特性
Features
- N-glycan sequencing of antibodies
- The enzymes provided are all active in a universal buffer system, GlycoBuffer 1, and can be used in single digests or in any combination.
- All reagents supplied are mass spectrometry compatible.
相关产品
相关产品
- Rapid PNGase F
- Rapid™ PNGase F (non-reducing format)
- PNGase F (Glycerol-free), Recombinant
单独销售的组分
- α2-3,6,8,9 Neuraminidase A
- α2-3 Neuraminidase S
- α1-3,4,6 Galactosidase
- β1-4 Galactosidase S
- β-N-Acetylglucosaminidase S
- α1-2,3,6 Mannosidase
- p0749a1246fucosidaseo

操作说明、说明书 & 用法

操作说明
1. Protocols for E0577
说明书
产品说明书包含产品使用的详细信息、产品配方和质控分析。
- manualE0577
应用实例
- Detailed Characterization of Antibody Glycan Structure using the N-Glycan Sequencing Kit

FAQs & 问题解决指南

FAQs
1. Is the N-Glycan Sequencing Kit protocol suitable for digestion of glycoproteins?
2. How do I calculate the molarity of released glycans from my antibody substrate?
3. Why are two different neuraminidase enzymes provided with the N-Glycan Sequencing Kit?
4. Should I use the one- or two-step Rapid PNGase F deglycosylation method for my antibody?
5. The deglycosylation protocol included with the N-Glycan Sequencing Kit is recommended for up to 100 µg of antibody; How do I determine the amount of starting material I should deglycosylate?
6. Is it possible to combine the α1-2,3,6 Mannosidase with other exoglycosidases? Are any of the exoglycosidases inhibited by the Zinc that is required for optimal mannosidase activity?
7. Does ammonium formate inhibit exoglycosidase digestion?
8. How long do I need to incubate a Rapifluor-labelled substrate with the exoglycosidases?
9. Which is the enzyme of choice to release α1-6 fucose residues from antibody glycans: α1-2,4,6 Fucosidase O or α1-2,3,4,6 Fucosidase from Bovine Kidney?
实验技巧

Remove all traces of Acetonitrile before digesting labeled substrate with exoglycosidases for optimal results.

Use PCR tubes to avoid evaporation when incubating reactions overnight.

Include ZnCl₂ in reactions with α1-2,3,6 Mannosidase (P0768) for optimal digestion.

Fucosidase O (P0749) requires an 18 hr incubation for complete digest of some instant labelled glycans and complex oligosaccharides with multiple branches.

If using Fucosidase O (P0749) alone, incubation temperature can be increased to 50°C for more efficient digestion.

引用 & 技术文献

产品手册
- Glycoproteomics Brochure
- Glycoproteomics Technical Guide
引用文献

产品引用文献查找工具

Powered by Bioz See more details on Bioz
出版物
- Guthrie, E.P., Magneilli, P.E. (2016) Using glycosidases to remove, trim, or modify glycans on therapeutic proteins BioProcess Int; 14(2), 32-37.
专题文章
- Using Glycosidases to Remove Trim or Modify Glycans on Therapeutic Protein
- Redesigning glycosidase manufacturing quality for pharmaceutical and clinical applications

质控、安全 & 法规

质控分析

每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。
产品说明与变更通知
产品说明与变更通知

产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]
- E0577S_v1
CoA
CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]
- E0577S_v1_10031280
- E0577S_v1_10039223
- E0577S_v1_10052370
- E0577S_v1_10068091
- E0577S_v1_10083878

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E. coli K12 ER2738

基因型

抗生素耐药性

生长基质

培养温度

Oligo (dT)25 Cellulose Beads

精选视频

Avoiding RNase Contamination

贮存溶液

产品引用文献查找工具

产品说明与变更通知

MfeI

精选视频

Reduce Star Activity with High-Fidelity Restriction Enzymes

产品来源

单位定义

反应条件

在不同缓冲液中的活性

稀释兼容性

贮存溶液

热失活

甲基化敏感性

同裂酶

相关产品

单独销售的组分

产品引用文献查找工具

产品说明与变更通知

N-Glycan Sequencing Kit

Features

相关产品

单独销售的组分

产品引用文献查找工具

产品说明与变更通知

Oligo (dT)₂₅ Cellulose Beads