LabAssay 碱性磷酸酶检测试剂盒

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  • 货号: 291-58601
    保存条件: 2-10℃
    供应商: 上海金畔生物科技有限公司·中国
    数量: 10
    英文名: LabAssay ALP
    保质期: 咨询
    规格: 900 tests
    Alkaline Phosphatase activity assay with p-Nitrophenylphosphate as a substrate

    Alkaline Phosphatase (ALP) is distributed in a variety of tissues such as liver, bone, and small intestine in animals. The change of the enzyme activity in tissues is an im-portant hallmark for physiological phenomena as osteo-genesis and so on. This kit is for Alkaline Phosphatase assay in a simultaneous multi-sample assay format with a microplate using p-Nitrophenylphosphate as a substrate.

    [Kit Contents]

    1. Substrate Tablet 20 tablets
      (p-Nitrophenylphosphate Disodium 6.7 mmol/L, after dissolving)
    2. Buffer Solution 100 mL
      (2.0 mmol/L MgCl2, 0.1mol/L Carbonate Buffer, pH 9.8)
    3. Stop Solution 100 mL(0.2 mol/L Sodium Hydroxide Solution)
    4. Standard Solution 10 mL
      (0.5 mmol/L p-Nitrophenol Solution)


    [Assay principle]
    p-Nitrophenylphosphate is hydrolyzed into p-Nitrophenol and Phosphoric Acid in the carbonate buffer (pH 9.8) in the presence of Alkaline Phosphatase in sample. Released p-Nitrophenol showing yellow color is optically measured at 405 nm wavelength as the enzyme activity.

    Dynamic assay range: > 0.06 mmol/L
    Standard assay range: 0 ~ 0.5 mmol/L (The activity at concentration more than 0.5 mmol/L can be determined by extrapolation due to its linearity.)
    Reproducibility: C.V. < 10%

    Yamamoto, M., Takahashi, Y., Tabata, Y.: Biomaterials, 24 (24), 4375 (2003). 



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