标签归档：gdna

Monarch® gDNA Wash Buffer |NEB酶试剂 New England Biolabs

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上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

产品信息

Monarch gDNA Wash Buffer is a component of the Monarch Genomic DNA Purification Kit (NEB #T3010). It is an ethanol-based, low salt buffer that allows for complete removal of chaotropic salt and cell components in just 2 wash steps, enabling the purification of highly-pure gDNA.

产品类别:
Genomic DNA Extraction & Purification Products,
RNA Extraction and Purification,
Nucleic Acid Purification Products

应用:
Nucleic Acid Purification

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • T3015L     25    
        Monarch® gDNA Wash Buffer T3015-2 25 1 x 60 ml Not Applicable

操作说明、说明书 & 用法

  • 操作说明

    1. Quick Protocol for Extraction and Purification of Genomic DNA
    2. Protocol for Extraction and Purification of Genomic DNA from Cells (NEB #T3010)
    3. Protocol for Extraction and Purification of Genomic DNA from Blood (NEB #T3010)
    4. Protocol for Extraction and Purification of Genomic DNA from Tissues (NEB #T3010)
    5. Protocol for Genomic DNA Cleanup (NEB #T3010)
    6. Genomic DNA Purification from Gram-positive Bacteria and Archaea (NEB #T3010)
    7. Genomic DNA Purification from Yeast (NEB #T3010)
    8. Genomic DNA Purification from Saliva (NEB #T3010)
    9. Genomic DNA Purification from Buccal Swabs (NEB #T3010)
    10. Genomic DNA Purification from Gram-negative Bacteria (NEB #T3010)

  • 使用指南

    • Choosing Input Amounts for the Monarch Genomic DNA Purification Kit
    • Factors Affecting DNA Quality when Purifying gDNA from Blood and Tissues with the Monarch Genomic DNA Purification Kit
    • Guidelines for Handling Tissue Samples when using the Monarch Genomic DNA Purification Kit

FAQs & 问题解决指南

  • FAQs

    1. What is the composition of each buffer provided with the Monarch® Genomic DNA Purification Kit?
    2. What size gDNA can be purified with the Monarch® Genomic DNA Purification Kit?
       

  • 问题解决指南

    • Troubleshooting Guide for Genomic DNA Extraction & Purification (NEB #T3010)

Monarch® gDNA 去除离心柱 |NEB酶试剂 New England Biolabs

发表于由

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

产品信息

Monarch® gDNA 去除离心柱 |

Monarch® gDNA 去除离心柱随 Monarch 总 RNA 小量提取试剂盒提供，可在总 RNA 小量提取流程中结合基因组 DNA，将其从提取的 RNA 溶液中去除。去除柱随附 Monarch 收集管 II（NEB #T2018），与真空歧管兼容。

Monarch® gDNA 去除离心柱 |

产品类别:
Total RNA Extraction & Purification Products,
RNA Extraction and Purification,
Nucleic Acid Purification Products

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • T2017L     25    
        Monarch® gDNA Removal Columns T2017-1 25 2 x 50 columns Not Applicable
        Monarch® Spin Collection Tubes T2118-1 25 2 x 50 tubes Not Applicable

  • 相关产品

    相关产品

    • Luna® 通用一步法 RT-qPCR 试剂盒
    • Luna® 通用探针一步法 RT-qPCR 试剂盒
    • e6560-protoscript-ii-first-strand-cdna-synthesis-kit
    • NEBNext® Ultra™ II RNA 非链特异性文库制备试剂盒
    • e7760-nebnext-ultra-ii-directional-rna-library-prep-kit-for-illumina
    • e7765-nebnext-ultra-ii-directional-rna-library-prep-with-sample-purification-beads
    • NEBNext® rRNA 去除试剂盒 – 人/小鼠/大鼠（含 RNA 纯化磁珠）
    • e7490-nebnext-polya-mrna-magnetic-isolation-module
    • m0368-protoscript-ii-reverse-transcriptase
    • t1010-monarch-plasmid-miniprep-kit
    • Monarch® DNA 胶回收试剂盒
    • Monarch® PCR & DNA 纯化试剂盒（5 μg）

    单独销售的组分

    • Monarch® RNA 提取离心柱
    • Monarch® DNA/RNA 保护试剂
    • Monarch® 总 RNA 小量提取试剂盒
    • Monarch® 收集管 II
    • Monarch® RNA 裂解液
    • Monarch® RNA 初始洗涤缓冲液
    • t2014-monarch-rna-wash-buffer
    • Monarch® 总 RNA 小量提取酶套装
    • Nuclease-free Water

操作说明、说明书 & 用法

  • 操作说明

    1. Quick Protocol for Total RNA Extraction (NEB #T2010)
    2. Download Quick Protocol Card T2010
    3. FFPE Tissue Deparaffinization and Subsequent RNA Purification Using the Monarch Total RNA Miniprep Kit  (NEB #T2010)
    4. Total RNA Purification from Cultured Mammalian Cells using the Monarch Total RNA Miniprep Kit (NEB #T2010)
    5. Total RNA Purification from Tissues and Leukocytes using the Monarch Total RNA Miniprep Kit (NEB #T2010)
    6. Total RNA Purification from Tough-to-Lyse Samples (bacteria, yeast, plant, etc.) using the Monarch Total RNA Miniprep Kit (NEB #T2010)

  • 使用指南

    • General Guidelines for Successful RNA Purification Using the Monarch Total RNA Miniprep Kit
    • Guidance on Choosing Sample Input Amounts when Using the Monarch Total RNA Miniprep Kit
    • Guidelines for Purification of RNA from Cultured Mammalian Cells
    • Guidelines for RNA Purification from Bacteria
    • Guidelines for RNA Purification from Leukocytes (WBCs, PBMCs)
    • Guidelines for RNA Purification from Plants
    • Guidelines for RNA Purification from Tissues 
    • Guidelines for RNA Purification from Whole Blood
    • Guidelines for RNA Purification from Yeast
    • Guidelines for Sample Stabilization Using the Monarch DNA/RNA Protection Reagent

FAQs & 问题解决指南

  • FAQs

    1. How can I tell the difference between the gDNA Removal Column and the RNA Purification Column?
    2. Can I skip the gDNA removal column step of the Monarch Total RNA Miniprep Kit protocol?
    3. Can the genomic DNA captured on the Monarch gDNA Removal Column (NEB #T2017) be eluted and used in downstream applications?
    4. Is the Monarch Total RNA Miniprep Kit compatible with Luna RT-qPCR Reagents?
    5. Is the Monarch Total RNA Miniprep Kit compatible with NEBNext reagents for RNA Library Prep?
    6. Can the Monarch Total RNA Miniprep Kit be used to purify RNA from bacteria?
    7. Can the Monarch Total RNA Miniprep Kit be used to purify RNA from plants?
    8. Can the Monarch Total RNA Miniprep Kit be used to purify RNA from yeast?
    9. What is the lowest volume in which I can elute my RNA with the Monarch Total RNA Miniprep Kit (NEB #T2010)?

  • 问题解决指南

    • Troubleshooting Guide for Total RNA Extraction & Purification

Monarch® gDNA Binding Buffer |NEB酶试剂 New England Biolabs

发表于由

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

产品信息

The Monarch gDNA Binding Buffer is a chaotropic salt-based binding buffer with low alcohol content, designed to optimize binding of gDNA to the spin column. The binding of gDNA takes place at low speed, maximizing binding and efficiency.  

产品类别:
Genomic DNA Extraction & Purification Products,
Nucleic Acid Purification Products

应用:
Nucleic Acid Purification

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • T3014L     25    
        Monarch® gDNA Binding Buffer T3014-2 25 1 x 65 ml Not Applicable

操作说明、说明书 & 用法

  • 操作说明

    1. Quick Protocol for Extraction and Purification of Genomic DNA
    2. Protocol for Extraction and Purification of Genomic DNA from Cells (NEB #T3010)
    3. Protocol for Extraction and Purification of Genomic DNA from Blood (NEB #T3010)
    4. Protocol for Extraction and Purification of Genomic DNA from Tissues (NEB #T3010)
    5. Protocol for Genomic DNA Cleanup (NEB #T3010)
    6. Genomic DNA Purification from Gram-positive Bacteria and Archaea (NEB #T3010)
    7. Genomic DNA Purification from Yeast (NEB #T3010)
    8. Genomic DNA Purification from Saliva (NEB #T3010)
    9. Genomic DNA Purification from Buccal Swabs (NEB #T3010)
    10. Genomic DNA Purification from Gram-negative Bacteria (NEB #T3010)

  • 使用指南

    • Choosing Input Amounts for the Monarch Genomic DNA Purification Kit
    • Factors Affecting DNA Quality when Purifying gDNA from Blood and Tissues with the Monarch Genomic DNA Purification Kit
    • Guidelines for Handling Tissue Samples when using the Monarch Genomic DNA Purification Kit

FAQs & 问题解决指南

  • FAQs

    1. What is the composition of each buffer provided with the Monarch® Genomic DNA Purification Kit?
    2. What size gDNA can be purified with the Monarch® Genomic DNA Purification Kit?
       

  • 问题解决指南

    • Troubleshooting Guide for Genomic DNA Extraction & Purification (NEB #T3010)

Monarch® gDNA Blood Lysis Buffer |NEB酶试剂 New England Biolabs

发表于由

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

产品信息

The Monarch gDNA Blood Lysis Buffer contains a strong protective component against the high nuclease activity in blood samples and supports rapid degradation of hemoglobin and other protein components.

产品类别:
Genomic DNA Extraction & Purification Products,
Nucleic Acid Purification Products

应用:
Nucleic Acid Purification

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • T3013L     25    
        Monarch® gDNA Blood Lysis Buffer T3013-2 25 1 x 20 ml Not Applicable

操作说明、说明书 & 用法

  • 操作说明

    1. Genomic DNA Purification from Buccal Swabs (NEB #T3010)
    2. Protocol for Extraction and Purification of Genomic DNA from Blood (NEB #T3010)
    3. Protocol for Extraction and Purification of Genomic DNA from Tissues (NEB #T3010)
    4. Genomic DNA Purification from Yeast (NEB #T3010)
    5. Genomic DNA Purification from Saliva (NEB #T3010)
    6. Protocol for Genomic DNA Cleanup (NEB #T3010)
    7. Genomic DNA Purification from Gram-negative Bacteria (NEB #T3010)
    8. Genomic DNA Purification from Gram-positive Bacteria and Archaea (NEB #T3010)
    9. Protocol for Extraction and Purification of Genomic DNA from Cells (NEB #T3010)
    10. Quick Protocol for Extraction and Purification of Genomic DNA

  • 使用指南

    • Choosing Input Amounts for the Monarch Genomic DNA Purification Kit
    • Factors Affecting DNA Quality when Purifying gDNA from Blood and Tissues with the Monarch Genomic DNA Purification Kit
    • Guidelines for Handling Tissue Samples when using the Monarch Genomic DNA Purification Kit

FAQs & 问题解决指南

  • FAQs

    1. What is the composition of each buffer provided with the Monarch® Genomic DNA Purification Kit?
    2. What size gDNA can be purified with the Monarch® Genomic DNA Purification Kit?
       
    3. Can I omit the RNase digestion step from the protocol? 
    4. Does it matter which anticoagulant is used in blood samples?

  • 问题解决指南

    • Troubleshooting Guide for Genomic DNA Extraction & Purification (NEB #T3010)