N-Glycopeptide Binding Protein |NEB酶试剂 New England Biolabs

上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。

产品信息

N-Glycopeptide Binding Protein, also known as Fbs1-GYR lectin, is engineered to selectively bind and enrich a wide variety of N-glycopeptides in a complex proteomic sample to assist N-glycoprotein identification and discovery, without requiring the use of traditional lectin affinity chromatography (LAC) techniques, HILIC columns or cartridges. This engineered lectin, originating from human Fbs1 protein, exhibits improved specificity towards high mannose, complex, and hybrid N-glycans, as well as towards fucosylated and non-fucosylated, sialylated and non-sialylated, with bi-, tri- and tetra-antennary structures. N-Glycopeptide Binding Protein includes a SNAP-tag for immobilizing to create versatile workflows.

产品来源

An E. coli strain that carries human Fbs1 mutant gene.

产品类别:
Glycobiology Products,
Proteome Analysis Products

应用:
Glycobiology & Proteomics,
Glycoprotein Analysis

  • 产品组分信息

    本产品提供以下试剂或组分:

    NEB # 名称 组分货号 储存温度 数量 浓度
    • P0872S     -20    
        N-Glycopeptide Binding Protein P0872SVIAL -20 1 x 1 ml 1 mg/ml

  • 特性和用法

    贮存溶液

    20 mM Tris-HCl
    50 mM NaCl
    0.2 mM TCEP
    pH 8

    热失活

    分子量

    理论上的: 45 kDa

    单位活性检测条件

    Functional Testing (NGlycan Enrichment) – A 200 μL reaction in 20 mM Tris-HCl, 50 mM NaCl, 0.2 mM TCEP, 10% acetonitrile, pH 8.0 containing 1 nmol of fluorescently labeled sialylglycopeptide and 100 μg NGlycopeptide Binding Protein incubated for 30 minutes at 4°C results in ≥40% enrichment as determined by UV-Vis Spectroscopy at 520 nm.

  • 优势和特性

    Features

    • Engineered lectin, a glycan-binding protein that recognizes a wide variety of N-glycoforms, for enrichment and identification of glycopeptides and glycoproteins
    • Selectively binds and enriches N-glycopeptides in a complex proteomics sample, unlike other lectin affinity chromatography (LAC) techniques, HILIC, or other lectin-based enrichment methods
    • Significantly increased glycopeptide spectrum match and glycoprotein ID and discovery
    • Versatile workflow

  • 相关产品

    相关产品

    • Trypsin-ultra™, Mass Spectrometry Grade
    • Endoproteinase LysC
    • SNAP-捕获磁珠
    • 6 孔磁性分离架

  • 参考文献

    1. Chen M. et al. (2017). An engineered high affinity Fbs1 carbohydrate binding protein for selective capture of N-glycans and N-glycopeptides. Nat Commun. May 23;8, 15487.
    2. Chen M., et al. (2023). Comparative site-specific N-glycoproteome analysis reveals aberrant N-glycosylation and gives insights into mannose-6-phosphate pathway in cancer. Commun Biol. Jan13;6(1), 48.
    3. Mersha F. et al. (2023). Defining the filarial N-glycoproteome by glycosite mapping in the human parasitic nematode Brugia malay. Sci Rep. May 16;13(1), 7951.

操作说明、说明书 & 用法

  • 操作说明

    1. N-Glycopeptide Binding Protein Typical Reaction Protocol (NEB #P8072)

FAQs & 问题解决指南

  • FAQs

    1. How does N-Glycopeptide Binding Protein improve glycoproteomic analysis?
    2. What is the specificity of N-Glycopeptide Binding Protein?
    3. How strong does the NGlycopeptide Binding Protein bind to a glycopeptide?
    4. Can N-Glycopeptide Binding Protein bind and enrich free glycans?
    5. Can N-Glycopeptide Binding Protein bind an intact glycoprotein?
    6. What influences the binding affinity of the N-Glycopeptide Binding Protein?

Monarch® gDNA Binding Buffer |NEB酶试剂 New England Biolabs

上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。

产品信息

The Monarch gDNA Binding Buffer is a chaotropic salt-based binding buffer with low alcohol content, designed to optimize binding of gDNA to the spin column. The binding of gDNA takes place at low speed, maximizing binding and efficiency.  

产品类别:
Genomic DNA Extraction & Purification Products,
Nucleic Acid Purification Products

应用:
Nucleic Acid Purification

  • 产品组分信息

    本产品提供以下试剂或组分:

    NEB # 名称 组分货号 储存温度 数量 浓度
    • T3014L     25    
        Monarch® gDNA Binding Buffer T3014-2 25 1 x 65 ml Not Applicable

操作说明、说明书 & 用法

  • 操作说明

    1. Quick Protocol for Extraction and Purification of Genomic DNA
    2. Protocol for Extraction and Purification of Genomic DNA from Cells (NEB #T3010)
    3. Protocol for Extraction and Purification of Genomic DNA from Blood (NEB #T3010)
    4. Protocol for Extraction and Purification of Genomic DNA from Tissues (NEB #T3010)
    5. Protocol for Genomic DNA Cleanup (NEB #T3010)
    6. Genomic DNA Purification from Gram-positive Bacteria and Archaea (NEB #T3010)
    7. Genomic DNA Purification from Yeast (NEB #T3010)
    8. Genomic DNA Purification from Saliva (NEB #T3010)
    9. Genomic DNA Purification from Buccal Swabs (NEB #T3010)
    10. Genomic DNA Purification from Gram-negative Bacteria (NEB #T3010)

  • 使用指南

    • Choosing Input Amounts for the Monarch Genomic DNA Purification Kit
    • Factors Affecting DNA Quality when Purifying gDNA from Blood and Tissues with the Monarch Genomic DNA Purification Kit
    • Guidelines for Handling Tissue Samples when using the Monarch Genomic DNA Purification Kit

FAQs & 问题解决指南

  • FAQs

    1. What is the composition of each buffer provided with the Monarch® Genomic DNA Purification Kit?
    2. What size gDNA can be purified with the Monarch® Genomic DNA Purification Kit?
       

  • 问题解决指南

    • Troubleshooting Guide for Genomic DNA Extraction & Purification (NEB #T3010)

pJN-RLBP (Coelenterazine-Binding Protein)


pJN-RLBP (Coelenterazine-Binding Protein)

品牌:Cosmo Bio
CAS No.:
储存条件:-20℃
纯度:
产品编号

(生产商编号)

等级 规格 运输包装 零售价(RMB) 库存情况 参考值

JNC-P-003

10 µg 咨询


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动,恕不另行通知,确切详情请联系上海金畔生物科技有限公司。

Acyl-CoA-binding Protein (human) ELISA Kit 酰基辅酶A结合蛋白 (人) ELISA试剂盒


Acyl-CoA-binding Protein (human) ELISA Kit

酰基辅酶A结合蛋白 (人) ELISA试剂盒

品牌:Adipogen
CAS No.:
储存条件:+4°C
纯度:
产品编号

(生产商编号)

等级 规格 运输包装 零售价(RMB) 库存情况 参考值

AG-45B-0019-KI01

96 wells 7,080.00


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动,恕不另行通知,确切详情请联系上海金畔生物科技有限公司。