上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
产品信息
Monarch® gDNA 去除离心柱随 Monarch 总 RNA 小量提取试剂盒提供,可在总 RNA 小量提取流程中结合基因组 DNA,将其从提取的 RNA 溶液中去除。去除柱随附 Monarch 收集管 II(NEB #T2018),与真空歧管兼容。
- 产品类别:
- Total RNA Extraction & Purification Products,
- RNA Extraction and Purification,
- Nucleic Acid Purification Products
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产品组分信息
本产品提供以下试剂或组分:
NEB # 名称 组分货号 储存温度 数量 浓度 -
T2017L 25 Monarch® gDNA Removal Columns T2017-1 25 2 x 50 columns Not Applicable Monarch® Spin Collection Tubes T2118-1 25 2 x 50 tubes Not Applicable
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相关产品
相关产品
- Luna® 通用一步法 RT-qPCR 试剂盒
- Luna® 通用探针一步法 RT-qPCR 试剂盒
- e6560-protoscript-ii-first-strand-cdna-synthesis-kit
- NEBNext® Ultra™ II RNA 非链特异性文库制备试剂盒
- e7760-nebnext-ultra-ii-directional-rna-library-prep-kit-for-illumina
- e7765-nebnext-ultra-ii-directional-rna-library-prep-with-sample-purification-beads
- NEBNext® rRNA 去除试剂盒 – 人/小鼠/大鼠(含 RNA 纯化磁珠)
- e7490-nebnext-polya-mrna-magnetic-isolation-module
- m0368-protoscript-ii-reverse-transcriptase
- t1010-monarch-plasmid-miniprep-kit
- Monarch® DNA 胶回收试剂盒
- Monarch® PCR & DNA 纯化试剂盒(5 μg)
单独销售的组分
- Monarch® RNA 提取离心柱
- Monarch® DNA/RNA 保护试剂
- Monarch® 总 RNA 小量提取试剂盒
- Monarch® 收集管 II
- Monarch® RNA 裂解液
- Monarch® RNA 初始洗涤缓冲液
- t2014-monarch-rna-wash-buffer
- Monarch® 总 RNA 小量提取酶套装
- Nuclease-free Water
操作说明、说明书 & 用法
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操作说明
- Quick Protocol for Total RNA Extraction (NEB #T2010)
- Download Quick Protocol Card T2010
- FFPE Tissue Deparaffinization and Subsequent RNA Purification Using the Monarch Total RNA Miniprep Kit (NEB #T2010)
- Total RNA Purification from Cultured Mammalian Cells using the Monarch Total RNA Miniprep Kit (NEB #T2010)
- Total RNA Purification from Tissues and Leukocytes using the Monarch Total RNA Miniprep Kit (NEB #T2010)
- Total RNA Purification from Tough-to-Lyse Samples (bacteria, yeast, plant, etc.) using the Monarch Total RNA Miniprep Kit (NEB #T2010)
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使用指南
- General Guidelines for Successful RNA Purification Using the Monarch Total RNA Miniprep Kit
- Guidance on Choosing Sample Input Amounts when Using the Monarch Total RNA Miniprep Kit
- Guidelines for Purification of RNA from Cultured Mammalian Cells
- Guidelines for RNA Purification from Bacteria
- Guidelines for RNA Purification from Leukocytes (WBCs, PBMCs)
- Guidelines for RNA Purification from Plants
- Guidelines for RNA Purification from Tissues
- Guidelines for RNA Purification from Whole Blood
- Guidelines for RNA Purification from Yeast
- Guidelines for Sample Stabilization Using the Monarch DNA/RNA Protection Reagent
FAQs & 问题解决指南
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FAQs
- How can I tell the difference between the gDNA Removal Column and the RNA Purification Column?
- Can I skip the gDNA removal column step of the Monarch Total RNA Miniprep Kit protocol?
- Can the genomic DNA captured on the Monarch gDNA Removal Column (NEB #T2017) be eluted and used in downstream applications?
- Is the Monarch Total RNA Miniprep Kit compatible with Luna RT-qPCR Reagents?
- Is the Monarch Total RNA Miniprep Kit compatible with NEBNext reagents for RNA Library Prep?
- Can the Monarch Total RNA Miniprep Kit be used to purify RNA from bacteria?
- Can the Monarch Total RNA Miniprep Kit be used to purify RNA from plants?
- Can the Monarch Total RNA Miniprep Kit be used to purify RNA from yeast?
- What is the lowest volume in which I can elute my RNA with the Monarch Total RNA Miniprep Kit (NEB #T2010)?
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问题解决指南
- Troubleshooting Guide for Total RNA Extraction & Purification