Next® 酶学转化法甲基化建库多样本接头引物试剂盒（Unique 双端 Index 引物） |NEB酶试剂 New England Biolabs

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产品信息

NEBNext 酶学转化法甲基化建库多样本接头引物试剂盒（Unique 双端 Index 引物）适用于 Illumina® 测序平台，包含 NEBNext EM-seq™ 接头和 NEBNext PCR 引物，可在文库扩增过程中引入 Unique 双端 Index 引物。

96 次反应的试剂盒含有一板一次性的密封着可穿刺的铝箔封板膜 96 孔板。96 孔板的每个孔都含有一对唯一的 i5 和 i7 Index 引物，因此共提供 96 种 Unique 双端 Index 引物。对于 24 次反应的试剂盒，Index 引物封装在 24 个单独的小管中，每个小管都含有一对独特的 i5 和 i7 Index 引物。24 次和 96 次反应试剂盒中提供的 i5 和 i7 Index 引物之间没有重复。

虽然 NEBNext EM-seq 接头针对 EM-seq 实验流程进行了优化，但同样适用于重亚硫酸盐测序实验流程。

NEBNext 酶学转化法甲基化建库试剂盒（NEB #E7120）也提供这些组份。

产品类别:
NEBNext® Multiplex Oligos (Adaptors & Primers) Products,
Epigenetic Analysis Products,
Epigenetics Products,

Next Generation Sequencing Library Preparation Products

应用:
DNA Methylation Analysis,
Bisulfite Conversion,
Epigenetics

  • 试剂盒组成

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • E7140S     -20    
        EM-seq™ Index Primer 1 (S) E7141AVIAL -20 1 x 0.005 ml 10 µM
        EM-seq™ Index Primer 2 (S) E7142AVIAL -20 1 x 0.005 ml 10 µM
        EM-seq™ Index Primer 3 (S) E7143AVIAL -20 1 x 0.005 ml 10 µM
        EM-seq™ Index Primer 4 (S) E7144AVIAL -20 1 x 0.005 ml 10 µM
        EM-seq™ Index Primer 5 (S) E7145AVIAL -20 1 x 0.005 ml 10 µM
        EM-seq™ Index Primer 6 (S) E7146AVIAL -20 1 x 0.005 ml 10 µM
        EM-seq™ Index Primer 7 (S) E7147AVIAL -20 1 x 0.005 ml 10 µM
        EM-seq™ Index Primer 8 (S) E7148AVIAL -20 1 x 0.005 ml 10 µM
        EM-seq™ Index Primer 9 (S) E7149AVIAL -20 1 x 0.005 ml 10 µM
        EM-seq™ Index Primer 10 (S) E7150AVIAL -20 1 x 0.005 ml 10 µM
        EM-seq™ Index Primer 11 (S) E7151AVIAL -20 1 x 0.005 ml 10 µM
        EM-seq™ Index Primer 12 (S) E7152AVIAL -20 1 x 0.005 ml 10 µM
        EM-seq™ Index Primer 13 (S) E7153AVIAL -20 1 x 0.005 ml 10 µM
        EM-seq™ Index Primer 14 (S) E7154AVIAL -20 1 x 0.005 ml 10 µM
        EM-seq™ Index Primer 15 (S) E7155AVIAL -20 1 x 0.005 ml 10 µM
        EM-seq™ Index Primer 16 (S) E7156AVIAL -20 1 x 0.005 ml 10 µM
        EM-seq™ Index Primer 17 (S) E7157AVIAL -20 1 x 0.005 ml 10 µM
        EM-seq™ Index Primer 18 (S) E7158AVIAL -20 1 x 0.005 ml 10 µM
        EM-seq™ Index Primer 19 (S) E7159AVIAL -20 1 x 0.005 ml 10 µM
        EM-seq™ Index Primer 20 (S) E7160AVIAL -20 1 x 0.005 ml 10 µM
        EM-seq™ Index Primer 21 (S) E7161AVIAL -20 1 x 0.005 ml 10 µM
        EM-seq™ Index Primer 22 (S) E7162AVIAL -20 1 x 0.005 ml 10 µM
        EM-seq™ Index Primer 23 (S) E7163AVIAL -20 1 x 0.005 ml 10 µM
        EM-seq™ Index Primer 24 (S) E7164AVIAL -20 1 x 0.005 ml 10 µM
        NEBNext® EM-seq™ Adaptor E7165AVIAL -20 1 x 0.06 ml Not Applicable
    • E7140L     -20    
        NEBNext® 96 Unique Dual Index Primer Pairs Plate (L) E7166AVIAL -20 1 x 5 μl/well 10 µM
        NEBNext® EM-seq™ Adaptor E7165AAVIAL -20 1 x 0.24 ml Not Applicable

操作说明、说明书 & 用法

  • 操作说明

    1. Where can I find protocols for using the NEBNext® Enzymatic Methyl-seq Kit? (NEB #E7120, E7125, E7140)

  • 说明书

    产品说明书包含产品使用的详细信息、产品配方和质控分析。

    • manualE7140

  • 使用指南

    • E7140L_E7120L_EMSeqUDI_IlluminaLRM
    • E7140S_E7120S_EMSeqUDI_IlluminaLRM
    • Forward Strand Workflow Sample Sheet for E7140L: NovaSeq 6000 with v1.0 reagent kits, MiniSeq with Rapid reagent kits, MiSeq, HiSeq 2000/2500 (paired-end flow cell), HiSeq 3000/4000 (single-read flow cell)
    • Forward Strand Workflow Sample Sheet for E7140S: NovaSeq 6000 with v1.0 reagent kits, MiniSeq with Rapid reagent kits, MiSeq, HiSeq 2000/2500 (paired-end flow cell), HiSeq 3000/4000 (single-read flow cell)
    • Reverse Complement Workflow Sample Sheet for E7140L: iSeq 100, MiniSeq with Standard reagent kits, NextSeq Systems, NovaSeq 6000 with v1.5 reagent kits, HiSeq 2000/2500 (single-read flow cell), HiSeq 3000/4000 (paired-end flow cell)
    • Reverse Complement Workflow Sample Sheet for E7140S: iSeq 100, MiniSeq with Standard reagent kits, NextSeq Systems, NovaSeq 6000 with v1.5 reagent kits, HiSeq 2000/2500 (single-read flow cell), HiSeq 3000/4000 (paired-end flow cell)

工具 & 资源

  • 选择指南

    • NEBNext® Multiplex Oligos Selection Chart

FAQs & 问题解决指南

  • FAQs

    1. What is provided in the NEBNext Multiplex Oligos for Enzymatic Methyl-seq?
    2. Can the NEBNext® Multiplex Oligos for Enzymatic Methyl-seq be used to address “index hopping” with Illumina patterned flow cells?
    3. How many indices are available with the 24 reaction- or 96 reaction-size of the NEBNext® Multiplex Oligos for Enzymatic Methyl-seq?
    4. Are the NEBNext® Multiplex Oligos for Enzymatic Methyl-seq the same as NEBNext® Multiplex Oligos for Illumina®?
    5. Are the EM-seq Adaptor and Index Primers validated in the EM-seq workflow?
    6. What is the concentration of the EM-seq Adaptor and Index Primers?
    7. How many reactions worth of primer are in each well/tube?
    8. Can I use the 96-well primer plate to do my PCR reactions?
    9. Do I need to spike in custom sequencing primers when sequencing libraries made with NEBNext® Multiplex Oligos for Enzymatic Methyl-seq on Illumina sequencing instruments?
    10. Can I perform single read runs and still get both index sequences? Can I run both single read and paired-end recipes with dual-indexed libraries?
    11. Are Sample Sheets available for use with the NEBNext® Multiplex Oligos for EM-seq?
    12. Can the EM-seq Adaptor be used for bisulfite sequencing?
    13. Are dual indexed libraries compatible with single end sequencing?
    14. What sequences need to be trimmed for NEBNext libraries that are sequenced on an Illumina instrument?