产品信息

• 产品组分信息

  本产品提供以下试剂或组分：

  NEB #	名称	组分货号	储存温度	数量	浓度
  P0872S     -20       N-Glycopeptide Binding Protein P0872SVIAL -20 1 x 1 ml 1 mg/ml

• 特性和用法

  贮存溶液

  20 mM Tris-HCl
  50 mM NaCl
  0.2 mM TCEP
  pH 8

  热失活

  否

  分子量

  理论上的: 45 kDa

  单位活性检测条件

  Functional Testing (N–Glycan Enrichment) – A 200 μL reaction in 20 mM Tris-HCl, 50 mM NaCl, 0.2 mM TCEP, 10% acetonitrile, pH 8.0 containing 1 nmol of fluorescently labeled sialylglycopeptide and 100 μg N–Glycopeptide Binding Protein incubated for 30 minutes at 4°C results in ≥40% enrichment as determined by UV-Vis Spectroscopy at 520 nm.

• 优势和特性

  Features

  • Engineered lectin, a glycan-binding protein that recognizes a wide variety of N-glycoforms, for enrichment and identification of glycopeptides and glycoproteins
  • Selectively binds and enriches N-glycopeptides in a complex proteomics sample, unlike other lectin affinity chromatography (LAC) techniques, HILIC, or other lectin-based enrichment methods
  • Significantly increased glycopeptide spectrum match and glycoprotein ID and discovery
  • Versatile workflow

• 相关产品

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• 参考文献

  1. Chen M. et al. (2017). An engineered high affinity Fbs1 carbohydrate binding protein for selective capture of N-glycans and N-glycopeptides. Nat Commun. May 23;8, 15487.
  2. Chen M., et al. (2023). Comparative site-specific N-glycoproteome analysis reveals aberrant N-glycosylation and gives insights into mannose-6-phosphate pathway in cancer. Commun Biol. Jan13;6(1), 48.
  3. Mersha F. et al. (2023). Defining the filarial N-glycoproteome by glycosite mapping in the human parasitic nematode Brugia malay. Sci Rep. May 16;13(1), 7951.

操作说明、说明书 & 用法

• 操作说明

  1. N-Glycopeptide Binding Protein Typical Reaction Protocol (NEB #P8072)

FAQs & 问题解决指南

• FAQs

  1. How does N-Glycopeptide Binding Protein improve glycoproteomic analysis?
  2. What is the specificity of N-Glycopeptide Binding Protein?
  3. How strong does the N–Glycopeptide Binding Protein bind to a glycopeptide?
  4. Can N-Glycopeptide Binding Protein bind and enrich free glycans?
  5. Can N-Glycopeptide Binding Protein bind an intact glycoprotein?
  6. What influences the binding affinity of the N-Glycopeptide Binding Protein?