产品信息

 

Q5 超保真 2X 预混液采用方便的预混液形式，且高效、高保真。Q5 超保真 2X 预混液是一种热稳定的高保真 DNA 聚合酶，具有 3´→5´ 核酸外切酶活性，并且融合了具有增强持续合成能力的 Sso7d 结构域，能够进行稳定的 DNA 扩增。Q5 超保真 DNA 聚合酶的错配率不到 Taq DNA 聚合酶的二百八十分之一，是克隆的理想选择，可用于长片段或难扩增片段的扩增。这款便捷的预混液剂型浓度为 2X。 本预混液包含 dNTP、Mg⁺⁺ 以及应用广泛的专有缓冲液，不论模板 GC 含量多少，仅需加入引物和 DNA 模板即可进行高效扩增。终浓度为 1X（推荐使用）时，Q5 超保真预混液含有 2 mM Mg⁺⁺。 Q5 超保真 DNA 聚合酶不同于常规的较低保真度的 PCR 酶。强烈建议使用 NEB T_m Calculator，确定给定引物组的最佳退火温度。

产品来源

大肠杆菌菌株，携带 Q5 超保真 DNA 聚合酶基因。

产品类别:

Q5® High-Fidelity DNA Polymerases Products,

Master Mixes Products

应用:

Long Range PCR,

Fast PCR,

High-Fidelity PCR,

Multiplex PCR,

Specialty PCR,

Routine PCR,

DNA Amplification, PCR & qPCR,

PCR,

Fast Cloning: Accelerate your cloning workflows with reagents from NEB

• 产品组分信息

  本产品提供以下试剂或组分：

  NEB #	名称	组分货号	储存温度	数量	浓度
  M0492S     -20       Q5® High-Fidelity 2X Master Mix M0492SVIAL -20 2 x 1.25 ml 2 X
  M0492L     -20       Q5® High-Fidelity 2X Master Mix M0492SVIAL -20 10 x 1.25 ml 2 X

• 特性和用法

  热失活

  否

• 优势和特性

  应用特性

  • 超保真 PCR
  • 克隆
  • 长片段或难扩增片段的扩增
  • 高通量 PCR

• 相关产品

  相关产品

  • dNTP 混合液
  • dNTP 套装
  • Q5® 超保真 DNA 聚合酶
  • Q5® 热启动超保真 2X 预混液
  • Q5® 热启动超保真 DNA 聚合酶
  • Q5® 反应缓冲液套装

• 注意事项

  1. A precipitate (most noticeable after the first 1–2 freeze/thaw cycles) is not uncommon. To ensure optimal performance, the master mix should be thawed and resuspended prior to use. Stability testing using up to 15 freeze/thaw cycles has shown no negative effect on master mix performance.
  2. Product specifications for individual components in the Q5 High-Fidelity 2X Master Mix are available separately.

• 参考文献

  1. Anastassia Voronova Erin Coyne, Ashraf Al Madhoun, Joel V. Fair, Neven Bosiljcic, Catherine St-Louis, Grace Li, Sherry Thurig, Valerie A. Wallace, Nadine Wiper-Bergeron, and Ilona S. Skerjanc. (2013). Hedgehog Signaling Regulates MyoD Expression and Activity. J Biol Chem. 288(6), 4389–4404. PubMedID: PMC3567689
  2. Lieve Naesens, Luke Guddat, Dianne Keough, André B.P. van Kuilenburg, Judith Meijer, Johan Vande Voorde and Jan Balzarini (2013). ROLE OF HUMAN HYPOXANTHINE GUANINE 
     PHOSPHORIBOSYLTRANSFERASE IN ACTIVATION 
     OF THE ANTIVIRAL AGENT T-705 (FAVIPIRAVIR). Molecular Pharmacology Fast Forward. 87247,
  3. Hicham Bouabe, Klaus Okkenhaug (2013). A Protocol for Construction of Gene Targeting Vectors and Generation of Homologous Recombinant Embryonic Stem Cells. Methods in Molecular Biology. 1064, 337-354.

操作说明、说明书 & 用法

• 操作说明

  1. Protocol for Q5® High-Fidelity 2X Master Mix

• 使用指南

  • Activity of Restriction Enzymes in PCR Buffers
  • Guidelines for PCR Optimization with Thermophilic DNA Polymerases

工具 & 资源

• 选择指南

  • DNA Polymerase Selection Chart

• Web 工具

  • Tm Calculator

FAQs & 问题解决指南

• FAQs

  1. What are the advantages of using Q5^® High-Fidelity DNA Polymerase?
  2. What are the differences between the numerous Q5^® Polymerase products available?
  3. My results are not as expected. Where can I find troubleshooting help?
  4. What ends will my PCR products have?
  5. What does exonuclease activity mean for a DNA polymerase?
  6. What are the properties of this polymerase (fidelity, product ends, max amplicon, modified base incorporation, etc.)?
  7. Does Q5^® High-Fidelity DNA Polymerase exhibit a strand displacement activity?
  8. How should I determine the appropriate annealing temperature for my reaction?
  9. What should the final primer concentration be in my PCR?
  10. When and how should I use the Q5^® High GC Enhancer?
  11. There is a precipitate in the bottom of the buffer tube. Is this normal?

• 问题解决指南

  • PCR Troubleshooting Guide