上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
产品信息
产品来源
Isolated from a strain of E. coli that carries the coding sequence for potato S. tuberosum apyrase (4).
- 产品类别:
- Phosphatases Products,
- RNA Modification
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产品组分信息
本产品提供以下试剂或组分:
NEB # 名称 组分货号 储存温度 数量 浓度 -
M0398S -20 Apyrase M0398SVIAL -20 1 x 0.02 ml 500 units/ml Apyrase Reaction Buffer B0398SVIAL -20 1 x 1.25 ml 10 X
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M0398L -20 Apyrase M0398LVIAL -20 1 x 0.1 ml 500 units/ml Apyrase Reaction Buffer B0398SVIAL -20 1 x 1.25 ml 10 X
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特性和用法
单位定义
One unit is defined as the amount of enzyme that catalyses the release of 1 μmol of inorganic phosphate from ATP (1 mM, NEB #P0756) in 1X Apyrase Reaction Buffer in 1 minute at 30°C in a total reaction of 50 μl.
反应条件
1X Apyrase Reaction Buffer
Incubate at 30°C1X Apyrase Reaction Buffer
20 mM MES
50 mM NaCl
5 mM CaCl2
1 mM DTT
0.05% Tween® 20
(pH 6.5 @ 25°C)贮存溶液
20 mM MES
50 mM NaCl
0.1 mM CaCl2
1 mM DTT
0.1% Tween® 20
50% Glycerol
pH 6.5 @ 25°C热失活
65°C for 20 minutes
分子量
实际: 47 kDa
比活度
3,000 units/mg
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优势和特性
Features
- Enzyme of highest purity, efficiency and quality
- Tested in updated range of more stringent quality controls
- Supplied at 10-fold higher concentration in the optimized storage buffer
- Supplied with new optimal reaction buffer
- Provided with updated, detailed characterization
- Discovered and characterized a new function: the ability to convert 5’ triphosphorylated RNA to monophosphorylated form, which can be ligated or removed by 5’ exonuclease
应用特性
- Highly efficient degradation of ATP to AMP.
- Removal of deoxynucleotides in DNA pyrosequencing between cycles (3).
- Conversion of 5´ triphosphorylated RNA to ligatable monophosphorylated form that can be used for 5´ RNA adaptor ligation.
- Conversion of 5´ triphosphorylated RNA to 5´ exonuclease XRN-1 (NEB #M0338) sensitive monophosphorylated RNA.
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相关产品
相关产品
- t1010-monarch-plasmid-miniprep-kit
- Monarch® DNA 胶回收试剂盒
- Monarch® PCR & DNA 纯化试剂盒(5 μg)
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注意事项
- Apyrase has a higher ratio of activity for ATP:ADP (14:1).
- Apyrase is a calcium-activated enzyme. It is approximately 50% active when Mg2+ substitutes Ca2+ in Apyrase Reaction Buffer.
- As a metal-dependent enzyme, Apyrase can be inhibited by EGTA and EDTA.
- The activity of Apyrase is approximately 30% in NEBuffers r1.1, r2.1, r3.1 and rCutSmart™ Buffer.
- Apyrase does not remove 5´ caps from eukaryotic mRNA.
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参考文献
- Kettlun, A.M. et al. (2005). Phytochemistry. 66, 975-982.
- Schildkraut, I., Schildkraut, E. and Barshevsky, T., New England Biolabs, Inc. Unpublished observation
- Hornung, V. et al . (2006). Science. 314, 994-997.
- Schildkraut, I. and Schildkraut, E., New England Biolabs, Inc. Unpublished observation
FAQs & 问题解决指南
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FAQs
- What is the activity of Apyrase in other NEBuffers?
- Can Mg2+ be substituted for Ca2+ in Apyrase reaction buffer?