Monarch® RNA 纯化洗涤缓冲液 |NEB酶试剂 New England Biolabs

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产品信息

Monarch® RNA 纯化洗涤缓冲液 |

Monarch RNA 纯化洗涤缓冲液是 Monarch RNA 纯化试剂盒（NEB #T2030、T2040、T2050）的组分，用于 RNA 结合后洗涤 RNA，以确保去除酶、NTP 和短 RNA 片段等污染物。该产品为高浓度 5X 缓冲液，使用前需要用乙醇（≥ 95%）稀释。该产品共 2 瓶，每瓶装有 20 ml 浓缩缓冲液。

产品类别:
RNA Cleanup Products,
RNA Extraction and Purification,
Nucleic Acid Purification Products

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • T2042L     25    
        Monarch® Buffer WX T2042-2 25 2 x 20 ml Not Applicable

操作说明、说明书 & 用法

  • 操作说明

    1. Purification of RNA from the Aqueous Phase Following TRIzol®/Chloroform Extraction using the Monarch® RNA Cleanup Kits
    2. Separation of Large and Small RNA into Fractions using the Monarch® RNA Cleanup Kits
    3. Extraction of RNA from Agarose Gels using the Monarch® RNA Cleanup Kits
    4. Monarch® RNA Cleanup Kit Protocol
    5. RNA Extraction from Cells Using the Monarch RNA Cleanup Kits
    6. RNA Extraction from Buccal/Nasopharyngeal Swabs Using the Monarch RNA Cleanup Kits
    7. RNA Extraction from Saliva Using the Monarch RNA Cleanup Kits
    8. Automation of Viral RNA Extraction from Saliva Using the Monarch RNA Cleanup Kit

  • 说明书

    产品说明书包含产品使用的详细信息、产品配方和质控分析。

    • manualT2030_T2040_T2050

  • 使用指南

    • Avoiding Ribonuclease Contamination
    • Guidelines for RNA Quantitation
    • Guidelines for Working with RNA During RNA Cleanup

FAQs & 问题解决指南

  • FAQs

    1. What is the composition of each buffer provided with the Monarch RNA Cleanup Kits?
    2. What factors affect my (A260/A230) when using the Monarch RNA Cleanup Kits?
    3. Can I purchase Monarch buffers and columns separately?
    4. Can extraction of RNA with the Monarch kits be automated?
    5. Can the Monarch RNA Cleanup Kits be used for RNA extraction?
    6. I noticed the buffer names have changed in the RNA Cleanup Kits. Are these buffers the same formulation as before?

  • 问题解决指南

    • Troubleshooting Guide for RNA Cleanup