产品信息

View or download extensive performance data below and in our Data Supplement
 
This new kit meets the need for substantially faster, more streamlined RNA library prep workflows that deliver high-quality directional libraries from a variety of sample types in a single day from total RNA. 

The kit allows the use of a single protocol for all RNA inputs (25 -250 ng total RNA) and incorporates master mixed reagents, reduced incubation times and fewer cleanup steps, reducing total time and consumables used.  

• 3-hour library prep enables single day total RNA to library, in conjunction with mRNA enrichment or rRNA depletion kits
• Fewer cleanups and pipetting steps reduce hands-on time
• A robust single protocol for all input amounts increases ease of use
• Flexibility is enabled with simple guidelines for customized protocols, if desired
• Automation-friendly, enabling scalability

Figure 1: NEBNext UltraExpress™RNA Library Prep Kit workflow

Figure 2: The NEBNext UltraExpress™ RNA Library Prep Kit produces high library yields across a range of inputs

Figure 3: The NEBNext UltraExpress™ RNA Library Prep Kit provides consistent transcript coverage

Figure 4: The NEBNext UltraExpress™ RNA Library Prep Kit provides greater sensitivity of transcript detection

Figure 5: The NEBNext UltraExpress™ RNA Library Prep Kit provides excellent transcript correlation between inputs and replicates
 

Figure 6: The NEBNext UltraExpress™ RNA Library Prep Kit provides excellent transcript correlation across sample types

产品类别:

Library Preparation for Illumina Products,

RNA Library Prep for Illumina,

Next Generation Sequencing Library Preparation Products

• 试剂盒组成

  本产品提供以下试剂或组分：

  NEB #	名称	组分货号	储存温度	数量	浓度
  E3330S     -20       NEBNext UltraExpress™ RNA Fragmentation Mix E3329AVIAL -20 1 x 0.096 ml 2 X   NEBNext UltraExpress™ First Strand Enzyme Mix E3331AVIAL -20 1 x 0.024 ml Not Applicable   NEBNext UltraExpress™ Strand Specificity Reagent E3332AVIAL -20 1 x 0.096 ml Not Applicable   NEBNext UltraExpress™ Second Strand Master Mix E3333AVIAL -20 1 x 0.72 ml Not Applicable   NEBNext UltraExpress™ End Prep Enzyme Mix E3334AVIAL -20 1 x 0.036 ml Not Applicable   NEBNext UltraExpress™ End Prep Reaction Buffer E3335AVIAL -20 1 x 0.06 ml Not Applicable   NEBNext UltraExpress™ Ligation Master Mix E3336AVIAL -20 1 x 0.288 ml Not Applicable   NEBNext UltraExpress™ USER Enzyme E3337AVIAL -20 1 x 0.048 ml Not Applicable   NEBNext® MSTC™ High Yield Master Mix E3338AVIAL -20 1 x 1.2 ml Not Applicable   NEBNext® Adaptor Dilution Buffer E7762AVIAL -20 1 x 2.4 ml Not Applicable   0.1X TE E3341AVIAL -20 1 x 2.4 ml Not Applicable   Nuclease-free Water E3342AVIAL -20 1 x 0.096 ml Not Applicable   NEBNext® Bead Reconstitution Buffer E3339AVIAL -20 1 x 0.096 ml Not Applicable
  E3330L     -20       NEBNext UltraExpress™ RNA Fragmentation Mix E3329AAVIAL -20 1 x 0.384 ml 2 X   NEBNext UltraExpress™ First Strand Enzyme Mix E3331AAVIAL -20 1 x 0.096 ml Not Applicable   NEBNext UltraExpress™ Strand Specificity Reagent E3332AAVIAL -20 1 x 0.384 ml Not Applicable   NEBNext UltraExpress™ Second Strand Master Mix E3333AAVIAL -20 3 x 0.96 ml Not Applicable   NEBNext UltraExpress™ End Prep Enzyme Mix E3334AAVIAL -20 1 x 0.144 ml Not Applicable   NEBNext UltraExpress™ End Prep Reaction Buffer E3335AAVIAL -20 1 x 0.24 ml Not Applicable   NEBNext UltraExpress™ Ligation Master Mix E3336AAVIAL -20 1 x 1.16 ml Not Applicable   NEBNext UltraExpress™ USER Enzyme E3337AAVIAL -20 1 x 0.192 ml Not Applicable   NEBNext® MSTC™ High Yield Master Mix E3338AAVIAL -20 1 x 4.8 ml Not Applicable   NEBNext® Adaptor Dilution Buffer E7762AAVIAL -20 1 x 9.6 ml Not Applicable   0.1X TE E3341AAVIAL -20 1 x 9.6 ml Not Applicable   Nuclease-free Water E3342AAVIAL -20 1 x 0.384 ml Not Applicable   NEBNext® Bead Reconstitution Buffer E3339AAVIAL -20 1 x 3.84 ml Not Applicable

• 特性和用法

  需要但不提供的材料

  • NEBNext Multiplex Oligos for Illumina®
  • NEBNext Multiplex Oligos options can be found at www.neb.com/oligos. Alternatively, customer supplied adaptor and primers can be used; please see information in link: https://www.neb.com/faqs/2019/03/08/can-i-use-this-nebnext-kit-with-adaptors-and-primers-from-other-vendors-than-neb
  • SPRIselect™ Reagent Kit (Beckman Coulter®, Inc. #B23317) or AMPure® XP Beads (Beckman Coulter, Inc. #A63881)
  • Magnetic Rack (NEB S1515S, Alpaqua® cat. #A001322, or equivalent)
  • 80% Ethanol (freshly prepared)
  • Thermal cycler
  • DNase-, RNase-free PCR strip tubes, for example TempAssure® PCR flex-free 8-tube strips (USA Scientific® #1402-4708)
  • Bioanalyzer® or TapeStation® (Agilent® Technologies, Inc.) and associated reagents and consumables

   

  For use with NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490):

  • 1.5 ml Microcentrifuge tube and NEB #S1506 Magnet stand or equivalent (for washing beads only)

   

  For use with NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) (NEB #E7400) and other NEBNext RNA depletion kits that do not include beads (NEB #E7750, E7850, E7865):

  • Agencourt® RNAClean® XP Beads (Beckman Coulter, Inc. #A63987)

• 相关产品

  相关产品

  • e7490-nebnext-polya-mrna-magnetic-isolation-module
  • E7400 NEBNext rRNA Depletion Kit v2 Human Mouse Rat
  • NEBNext^® 多样本接头引物试剂盒 5（96 种 Unique 双端 Index 引物）
  • NEBNext^® 文库定量试剂盒（Illumina^®）
  • NEBNext^® 磁性分离架
  • T2030 Monarch RNA Cleanup Kit 10 ug
  • Monarch^® 总 RNA 小量提取试剂盒

操作说明、说明书 & 用法

• 操作说明

  1. Where can I find protocols for using the NEBNext UltraExpress™ RNA Library Prep Kit (NEB #E3330)?

• 说明书

  产品说明书包含产品使用的详细信息、产品配方和质控分析。

  • manualE3330

工具 & 资源

• Web 工具

  • NEBNext Selector

FAQs & 问题解决指南

• FAQs

  1. Is the NEBNext UltraExpress™ RNA library prep kit a directional (stranded) library kit?
  2. What is the difference between the NEBNext UltraExpress™ RNA Library Prep Kit and the NEBNext® Ultra™ II Directional RNA Library Prep Kit? How do I choose the right one?
  3. Is it possible to use total RNA input amounts outside the suggested range for the NEBNext UltraExpress™ RNA library Kit?
  4. Does the NEBNext UltraExpress™ RNA Library Prep Kit include poly(A) mRNA isolation or rRNA Depletion reagents?
  5. Do the NEBNext UltraExpress™ Library Prep kits come with beads?
  6. Can I perform poly(A) mRNA isolation or rRNA depletion and then freeze my samples at -80°C before beginning library preparation with the NEBNext UltraExpress™ RNA Library Prep Kit?
  7. Which NEBNext^® Multiplex Oligos (Adaptors & Primers) can be used with the NEBNext UltraExpress Library Prep Kits?
  8. What should I do if I see adaptor dimer in my NEBNext UltraExpress™ libraries?
  9. Can this kit be used with adaptors and primers from suppliers other than NEB?
  10. Are the USER enzyme included in the NEBNext UltraExpress™ RNA Library Prep Kit (blue cap) and the USER enzyme included with NEBNext Index Primer sets (red cap) the same?
  11. What sample types and quality are compatible with the NEBNext UltraExpress™ RNA Library Prep Kit?
  12. What do I do if I see a precipitate in the Ultra II End Prep Reaction Buffer?