标签归档：resin

Amylose Resin |NEB酶试剂 New England Biolabs

发表于由

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

产品信息

An affinity matrix used for the isolation of proteins fused to maltose-binding protein. It is intended for use in a gravity flow column.

For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact [email protected] for further information.

产品类别:
Amylose Purification (MBP-tag),
Affinity Purification Products,
Protein Purification Products

应用:
MBP Affinity Tag,
Affinity Purification & Expression Tags

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • E8021V     4    
        Amylose Resin E8021VVIAL 4 1 x 10 ml Not Applicable
    • E8021S     4    
        Amylose Resin E8021SVIAL 4 1 x 15 ml Not Applicable
    • E8021L     4    
        Amylose Resin E8021LVIAL 4 1 x 100 ml Not Applicable

  • 特性和用法

    贮存溶液

    20% Ethanol

    结合容量

    >4mg MBP5*-paramyosin ΔSal fusion protein/ml amylose resin.

    可再生

    Yes

  • 注意事项

    1. Store at 4°C. After use, resin should be stored incolumn buffer plus 0.02% sodium azide or 20% ethanol.
    2. Amylose Resin column should be washed with 5 volumes ofcolumn buffer before each use.
    3. For optimum performance, load crude extract at < 60cm/hour.
    4. When regenerating the column at 4°C, please note that0.1% SDS can precipitate at that temperature. It is therefore recommended thatthe SDS solution be stored at room temperature until needed. The resin may begenerated up to five times.
    5. For a complete affinity purification protocol, download the pMAL Protein Fusion and Purification System technical bulletin (NEB #E8200) from www.neb.com.
    6. Regeneration: The packed resin may beregenerated by the following wash sequence: Water 3 – column volumes, 0.1% SDS -3 column volumes, Water – 1 column volume, Column Buffer – 5 columnvolumes.

工具 & 资源

  • 选择指南

    • Purification Beads, Columns and Resins

FAQs & 问题解决指南

  • FAQs

    1. Much of my fusion protein flows through the amylose column. Is there anything I can do to improve my fusion’s affinity for the amylose column?
    2. How many times can I use the amylose column?
    3. What is known about binding in the presence of nonionic detergents?
    4. Can I substitute a different buffer and/or salt concentration in the Column Buffer?
    5. I see my intact fusion protein by SDS-PAGE when I run cells boiled in Sample Buffer, but when I check the crude extract the fusion is degraded.
    6. When I run my purified fusion protein on SDS-PAGE, why do I see multiple bands instead of a single band of the expected MW?
    7. Can I perform a batch purification using the amylose resin?
    8. Can MBP fusions be purified in the presence of denaturants like urea or guanidine-HCl?
    9. Is the amylose resin damaged by storage at -20°C?
    10. What gravity column do you recommend?
    11. Can amylose resin be cleaned, stored, and used again?

Amylose Resin High Flow |NEB酶试剂 New England Biolabs

发表于由

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

产品信息

Amylose Resin High Flow is a cross-linked affinity matrix used for the isolation of proteins fused to maltose-binding protein (MBP). This rigid matrix can be used in automated chromatography systems.

DNASU is a central repository for plasmid clones and collections that may also be helpful.

For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact [email protected] for further information.

产品类别:
Amylose Purification (MBP-tag),
Affinity Purification Products,
Protein Purification Products

应用:
MBP Affinity Tag,
Affinity Purification & Expression Tags,
Protein Purification,

Phage Display

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • E8022S     4    
        Amylose Resin High Flow E8022SVIAL 4 1 x 15 ml Not Applicable
    • E8022L     4    
        Amylose Resin High Flow E8022LVIAL 4 1 x 100 ml Not Applicable

  • 特性和用法

    贮存溶液

    20% Ethanol

    结合容量

    >4 mg MBP5* -paramyosin δSal fusion protein / ml amylose high flow resin.

    可再生

    Yes

  • 注意事项

    1. Store at 4°C. After use, resin should be stored in column buffer plus 0.02% sodium azide or 20% ethanol.
    2. Amylose Resin column should be washed with 5 volumes of column buffer before each use. 
      For optimum performance, load crude extract at < 60 cm/hour.
    3. When regenerating the column at 4°C, please note that 0.1% SDS can precipitate at that temperature. It is therefore recommended that the SDS solution be stored at room temperature until needed. The resin may be generated up to five times. 
    4. For a complete affinity purification protocol, download the pMAL Protein Fusion and Purification System technical bulletin (NEB #E8200) from www.neb.com.
    5. Regeneration: The packed resin may be regenerated by the following wash sequence: Water 3 – column volumes, 0.1% SDS – 3 column volumes, Water – 1 column volume, Column Buffer – 5 column volumes.

工具 & 资源

  • 选择指南

    • Purification Beads, Columns and Resins

FAQs & 问题解决指南

  • FAQs

    1. What gravity column do you recommend?
    2. Much of my fusion protein flows through the amylose column. Is there anything I can do to improve my fusion’s affinity for the amylose column?
    3. How many times can I use the amylose column?
    4. What is known about binding in the presence of nonionic detergents?
    5. Can I substitute a different buffer and/or salt concentration in the Column Buffer?
    6. I see my intact fusion protein by SDS-PAGE when I run cells boiled in Sample Buffer, but when I check the crude extract the fusion is degraded.
    7. When I run my purified fusion protein on SDS-PAGE, why do I see multiple bands instead of a single band of the expected MW?
    8. Can I perform a batch purification using the amylose resin?
    9. Can MBP fusions be purified in the presence of denaturants like urea or guanidine-HCl?
    10. Is the amylose resin damaged by storage at -20°C?
    11. What is the recommended flow rate and maximum pressure for Amylose Resin High Flow once packed into a column?

Express Ni Resin |NEB酶试剂 New England Biolabs

发表于由

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

产品信息

NEBExpress® Ni Resin is an affinity matrix for the isolation and purification of polyhistidine-tagged (His-tagged) fusion proteins. It is intended for use in gravity or pressure flow columns, and batch purifications. NEBExpress Ni Resin is comprised of a highly uniform and stable chemical-tolerant resin, pre-charged with nickel ions on the matrix surface.  It is resistant to a wide range of chemicals, including NaOH, EDTA, DTT and β-Mercaptoethanol.

For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact [email protected] for further information.

Support Matrix: Spherical, agarose based microparticles ranging in size from 10-100 μm.
产品类别:
Amylose Purification (MBP-tag),
NEBExpress MBP Fusion and Purification System,
Bacterial E. coli Protein Expression Products,

Nickel Purification (His-tag),
Affinity Purification Products,
Protein Purification Products,

Protein Expression Products

应用:
MBP Affinity Tag,
Fusion Protein Cleavage,
Pull Down Assays,

His-tagged Protein Expression & Purification,
Affinity Purification & Expression Tags,
Protein Purification,

Protein Analysis Tools

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • S1428S     4    
        NEBExpress® Ni Resin S1428SVIAL 4 1 x 25 ml 50 %

  • 特性和用法

    贮存溶液

    20% Ethanol

    支持介质

    Spherical, agarose based microparticles ranging in size from 10-100 μm.

    结合容量

    1 ml of NEBExpress™ Ni Resin will bind ≥ 10 mg of His-tagged fusion protein.

  • 优势和特性

    Features

    • Isolation and purification of His-tagged fusion proteins under native or denaturing conditions as IMAC tolerates a wide range of conditions, including the presence of protein denaturants, reducing agents and detergents.
    • High specific binding of His-tagged proteins from various expression systems yielding milligram quantities of target protein with purities of >95%.
    • Can be used with common cell lysis reagents and a variety of buffer additives

  • 相关产品

    相关产品

    • NEBExpress® Ni-NTA 磁珠
    • TEV Protease
    • Amylose Resin
    • Amylose Resin High Flow
    • 几丁质树脂
    • NEBExpress® Ni Spin Columns

  • 注意事项

    1. Do not freeze.
    2. Several E. coli proteins are capable of binding immobilized metal affinity matrices with moderate to high affinity. The most common contaminants include SlyD (28kDa), GlmS (67kDa), ArnA (74kDa) and carbonic anhydrase (25 kDa) (1). The named contaminants may be eliminated from the Ni column elution fraction by employing NiCo21(DE3) (NEB# C2529H) as the expression host and then performing a second binding step with chitin magnetic beads (NEB# E8036) (2). 
    3. Protein yield and purity are dependent upon the expression level, conformation and solubility characteristics of the recombinant fusion protein.  For best results, estimate the expression level of the His-tagged protein of interest by first running a sample of the crude lysate on an SDS-PAGE gel. 
    4. Unlike most IMAC resins, the unique chemistry employed in NEBExpress™ Ni Resin provides chemical tolerance to chelating agents such as EDTA and reducing agents such as DTT.  Please consult the chemical compatibility table for more information. 
    5. Polyhistidine tags do not typically compromise the biological protein function and are not considered immunogenic, however if cleavage of the His-tag is necessary we recommend using TEV Protease (NEB# P8112).
    6. Ni resin yields highly variable binding capacities dependent on the target and conditions. The binding capacity value (≥ 10 mg/ml) for this product was determined by performing a mock purification from crude cell lysates expressing a His-tagged target protein.

  • 参考文献

    1. Bolanos-Garcia et al (2006). BBA. 1760, 1304-1313.
    2. Samuelson, J (2016). GEN. 26-27.

操作说明、说明书 & 用法

  • 操作说明

    1. NEBExpress® Ni Resin Gravity Flow Typical Protocol (NEB #S1428)
    2. NEBExpress® Ni Resin Batch Binding Typical Protocol (NEB #S1428)
    3. NEBExpress® Ni Resin Pressurized Column Typical Protocol (NEB #S1428)
    4. NEBExpress® Ni Resin Quick Start Protocol (NEB #S1428)
    5. His-tag removal from protein using TEV Protease

工具 & 资源

  • 选择指南

    • Purification Beads, Columns and Resins

FAQs & 问题解决指南

  • FAQs

    1. Can NEBExpress® Ni Resin be used in large scale, pressurized purification systems such as FPLC?
    2. Will extended binding increase the yield of the target protein in the eluate?
    3. Does NEBExpress Ni Resin need to be regenerated with nickel after cleaning?
    4. How can I reduce contaminating proteins in a protein purification when using Ni Resin?
    5. What are the recommendations for pH based elution, as opposed to imidazole?
    6. How can I remove imidazole from a protein sample?
    7. What are to the advantages of performing purification under denaturing conditions?
    8. How can I determine if my target protein remains bound to the resin?
    9. Why is imidazole not necessary in the lysis/binding buffer? Why does the wash buffer contain only 5 mM imidazole?
    10. Why is my protein precipitating on the column?
    11. What gravity column do you recommend?
    12. How can I determine if my target protein remains bound to the resin?

  • 问题解决指南

    • Troubleshooting Guide for Purification using NEBExpress® Ni Resin

Strong Acidic Cation Exchange Resin No.3 (4% Cross-linking, 100-200 Mesh, H Type)

发表于由

Strong Acidic Cation Exchange Resin No.3 (4% Cross-linking, 100-200 Mesh, H Type)

品牌：FUJIFILM Wako
CAS No.：69011-20-7
储存条件：室温
纯度：–
产品编号

(生产商编号)

 等级 规格 运输包装 零售价(RMB) 库存情况 参考值

358-45671

 – 100mL – 咨询 – –


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