Amylose Resin |NEB酶试剂 New England Biolabs

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产品信息

An affinity matrix used for the isolation of proteins fused to maltose-binding protein. It is intended for use in a gravity flow column.

For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact [email protected] for further information.

产品类别:
Amylose Purification (MBP-tag),
Affinity Purification Products,
Protein Purification Products

应用:
MBP Affinity Tag,
Affinity Purification & Expression Tags

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • E8021V     4    
        Amylose Resin E8021VVIAL 4 1 x 10 ml Not Applicable
    • E8021S     4    
        Amylose Resin E8021SVIAL 4 1 x 15 ml Not Applicable
    • E8021L     4    
        Amylose Resin E8021LVIAL 4 1 x 100 ml Not Applicable

  • 特性和用法

    贮存溶液

    20% Ethanol

    结合容量

    >4mg MBP5*-paramyosin ΔSal fusion protein/ml amylose resin.

    可再生

    Yes

  • 注意事项

    1. Store at 4°C. After use, resin should be stored incolumn buffer plus 0.02% sodium azide or 20% ethanol.
    2. Amylose Resin column should be washed with 5 volumes ofcolumn buffer before each use.
    3. For optimum performance, load crude extract at < 60cm/hour.
    4. When regenerating the column at 4°C, please note that0.1% SDS can precipitate at that temperature. It is therefore recommended thatthe SDS solution be stored at room temperature until needed. The resin may begenerated up to five times.
    5. For a complete affinity purification protocol, download the pMAL Protein Fusion and Purification System technical bulletin (NEB #E8200) from www.neb.com.
    6. Regeneration: The packed resin may beregenerated by the following wash sequence: Water 3 – column volumes, 0.1% SDS -3 column volumes, Water – 1 column volume, Column Buffer – 5 columnvolumes.

工具 & 资源

  • 选择指南

    • Purification Beads, Columns and Resins

FAQs & 问题解决指南

  • FAQs

    1. Much of my fusion protein flows through the amylose column. Is there anything I can do to improve my fusion’s affinity for the amylose column?
    2. How many times can I use the amylose column?
    3. What is known about binding in the presence of nonionic detergents?
    4. Can I substitute a different buffer and/or salt concentration in the Column Buffer?
    5. I see my intact fusion protein by SDS-PAGE when I run cells boiled in Sample Buffer, but when I check the crude extract the fusion is degraded.
    6. When I run my purified fusion protein on SDS-PAGE, why do I see multiple bands instead of a single band of the expected MW?
    7. Can I perform a batch purification using the amylose resin?
    8. Can MBP fusions be purified in the presence of denaturants like urea or guanidine-HCl?
    9. Is the amylose resin damaged by storage at -20°C?
    10. What gravity column do you recommend?
    11. Can amylose resin be cleaned, stored, and used again?