Hampton 膜蛋白结晶试剂 MembFac • Crystal Screen Lite • MembFac HT

Hampton  MembFac • Crystal Screen Lite • MembFac HT

Applications
Primary sparse matrix crystallization screen for membrane proteins and samples with limited solubility   膜蛋白和样品溶解度有限样品
Features
  Membrane protein sparse matrix screens
  Sparse matrix formula efficiently samples salts, polymers, organics and pH
  pH range 4.6 – 8.5
  Formulated for use with detergents
   Tube or Deep Well block format
   Crystal Screen Lite features a lower ionic strength than the original Crystal Screen

Description
MembFac is a highly effective sparse matrix screen specifically designed as a preliminary screen for the crystallization of membrane proteins. MembFac is based upon the highly effective biased sparse matrix methodology. The MembFac screen matrix is optimized for the crystallization of membrane proteins.
MembFac contains 48 unique reagent formulations. With this set of 48 conditions, numerous chemicals and chemical combinations are utilized, allowing one to evaluate a large variety of potential crystallization conditions. MembFac covers a broad pH range between 4 and 9.
The Rationale
The basic rationale of MembFac is to perform a sparse matrix screen while changing the detergent dimension because detergents play an important factor in the crystallization of membrane proteins. A MembFac screen is to be completed for each detergent. Detergent screens are available separately.
The Method
The membrane protein of interest is first isolated in the detergent which gives the highest stability and activity. The protein concentration should be approximately 10 to 20 mg/ml and the detergent concentration should be only slightly above the CMC. The sample is then set against MembFac, using all 48 solutions plus the screening detergent(s) of choice. The screening detergent concentration should be approximately one to three times the CMC. Hence, for each detergent screened, one would utilize approximately 1 to 2 mg of sample.
The MembFac protocol was used to crystallize membrane proteins including SQR, fumerate: quinone oxireductase, LHII, and LHCII.
Crystal Screen Lite is a sparse matrix of trial crystallization reagent conditions based upon the Crystal Screen™.3 The primary screen variables are salt, pH, and precipitant (salts, polymers, volatile organics, and non-volatile organics).2Crystal Screen Lite differs from the original Crystal Screen kit in that the primary precipitant reagents are one-half the concentration of that used in the original Crystal Screen formulation. The secondary salts, ions, and buffers remain at the original Crystal Screen concentration. Reducing the primary concentration of the primary precipitant results in a screen which is “more gentle” on the sample and typically produces much less precipitate conditions than the original Crystal Screen.
MembFac contains 48 unique reagents, 10 ml each.
MembFac HT contains 1 ml each of all 48 reagents from MembFac and reagents 1-48 from Crystal Screen Lite in a single Deep Well block format.
Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

CAT NO NAME DESCRIPTION
HR2-114 MembFac 10 ml, tube format
HR2-128 Crystal Screen Lite 10 ml, tube format
HR2-137 MembFac HT 1 ml, Deep Well block format

Hampton蛋白悬滴结晶板 HDP Hanging Drop Crystallization Plate

Hampton蛋白悬滴结晶板 HDP Hanging Drop Crystallization Plate
美国Hampton Research公司位于加利福尼亚州,是一家专门从事蛋白质晶体研究的生产商。该公司向全球科研人员提供全面的晶体研究试剂和相关实验耗材,同时提供晶体设计和合成方面的定制服务。Hampton Research公司以其先进的生物大分子结晶技术和广泛实用的产品线,不仅为全世界晶体研究人员的工作带来了便捷,而且也受益于科研人员的回馈不断发展自己,目前已成为晶体研究领域最受信赖的品牌之一。
Hampton research主要产品:
Crystallization Screens 用于筛选和优化蛋白质/多肽/核酸结晶的一系列经验证的高精配方型溶液
Optimize Reagents 结晶级别盐类,聚合物,有机溶液或缓冲液
Crystallization Plates, Hardware & Accessories 结晶板(悬滴法和坐滴法,微量透析,微配液,自由界面扩散,凝胶以及无容器悬浮法)及其他配件
Cryocrystallography 低温晶体学耗材如 Crystalcap system,cryoloops
Goniometer Heads&Supplies 测角器头和配件
Protein Crystallization Standards 结晶级别标准蛋白
Custom Shop Crystallization Reagents 定制试剂盒和结晶溶液内的单一组分
StockOptions Kits 内含盐类,缓冲液和冷冻液的简便型试剂盒
Tools,Seeding&Resin 结晶用探针,结晶笔,seeding法结晶工具
Capillary Mounts & Supplies 毛细管,玻璃纤维, 蜡,黏合剂和密封剂
Hampton蛋白悬滴结晶板 HDP Hanging Drop Crystallization Plate
HDP Hanging Drop Crystallization Plate (Swissci)
HDP Hanging Drop Plate
HDP Hanging Drop Crystallization Plate (Swissci)
Single Well with Tool
HDP Hanging Drop Crystallization Plate (Swissci)
Hanging Drop Wells
Applications—Hanging drop crystallization
Features
  Made from optically superior polymer (UVP)
  Allen key novel crystal plug closure system
  Wells are micro-numbered
Description  描述:
The Swissci Hanging Drop Crystallization Plate is a breakthrough for protein crystallization in a 96-well format. For the first time individual wells can be identified and removed without any disturbance to the growing crystals inside the plate.Description
The plate has been developed by Swissci AG together with leading Crystallographers. It is the result of many years of experience in successful robotic high-throughput crystallization.
The Hanging Drop Crystallization Plate offers unique properties that make it ideal for both nanoliter crystallization screening and microliter optimization alike. Made from optically superior polymer (UVP) and with a new design of the wells, the plate allows easy crystal viewing and retrieval. The system is fully covered by design and patent protection.
The Advantages of the Hanging Drop Crystallization Plate
Easy Crystal Retrieval
Raised wide wells make the crystal mounting especially easy. Allen key system allows for smooth removal and subsequent microscopic investigation / X-ray with the novel crystal plug closure all included in the system.
Easy Viewing
The wells are wide conical and have a lens effect for perfect illumination. The micro-numbering ensures you will never loose the location under the microscope.
The optically superior polymer is UV transmissible and can be used to differentiate between salt and protein crystals.
Integral Sealing
Wide partition walls between each well provides a high surface area for very good sealing with the advanced quality sealing tape. The sealing tape is integrated into the kit so there is no need to purchase additional parts prior to starting a procedure.
Wide Range of Volumes
Typical volumes are 50 – 200 µl of reservoir and 10 nanoliter – 5 microliter drop size.
SBS Standard
The complete plate is designed to the 96-well SBS standard for all common holders.

CAT NO NAME DESCRIPTION
HR3-187 Hanging Drop Crystallization Plate (Swissci) 10 plate case

Hampton蛋白悬滴结晶板 HDP Hanging Drop Crystallization Plate 相关产品:

96 Well Hanging Drop Vapor Diffusion Plate
Hanging drop crystallization
ARI Hanging Drop Seals for 96 Well Plates
ARI Hanging Drop Seals for 96 Well Plates
CrystalQuick 96 Well Sitting Drop Plate (Greiner)
Sitting drop crystallization
Detergent Screen HT
96 detergents Deep Well block format
Glass Cover Slide Gizmo Dispenser
Cover Slide Dispenser
Greiner ComboPlate and CrystalBridge
Hanging and sitting drop crystallization
HDP Hanging Drop Crystallization Plate (Swissci)
Hanging Drop Crystallization
Linbro Plate
Hanging drop crystallization
ProCrystal
Protein Crystallization Covers for Hanging Drop Vapor Diffusion
Protein Methods
Daniel M. Bollag, Michael D. Rozycki, & Stuart J. Edelstein
Solubility & Stability Screen
Identify protein specific additives that promote solubility and stability
UVP Hanging Drop Plate Seal (Swissci)
Swissci UVP Hanging Drop Plate Seal
VDX Plate with sealant
Hanging drop crystallization
VDX Plate without sealant
Hanging drop crystallization
VDX48 Plate with sealant
Hanging drop crystallization
VDXm Plate without sealant
Hanging drop crystallization
VDXm Plate with sealant
Hanging drop crystallization

 

Hampton 蛋白结晶向导管Wizard Tube

Wizard Tube 
The Wizard™ Classic line of random sparse matrix screens is designed to increase your probability of producing crystals during the coarse screening phase when crystallizing biological macromolecules (proteins, nucleic acids, peptides, and combinations thereof).
The Wizard Classic reagents are proven to be a highly effective starting point in the screening of biological macromolecules. The Wizard Classic formulations include a large range of crystallants, buffers, and salts covering a broad range of crystallization space at pH levels from pH 4.5 to pH 10.5.Choose from Wizard Classic 1, 2, 3, or 4 non-overlapping formulations in matrix blocks or tubes.

图片
Wizard Cubic LCP
The tools in the Wizard Cubic LCP (lipidic cubic phase) Kit enable researchers to prepare LCP-type crystallizations by hand. Ideal for low-protein experiments: effective protein volume for a single crystallization experiment is about 80 nanoliters. Wizard Cubic LCP Kit tools work especially well when traditional methods have failed to yield crystals. Lipidic cubic phase has worked well for the crystallization of 7TM membrane proteins (proteins with seven transmembrane helices). Four out of six GPCRs (G-protein coupled receptors), an important membrane protein class, and several microbial 7TM proteins have been crystallized using the LCP approach.
图片
CRYO (I、II)
The Wizard Cryo™ line of random sparse matrix screens is designed for scientists who want to avoid the additional step of optimizing a cryoprotectant condition. Every Wizard Cryo formulation flash-freezes to a clear, amorphous glass in liquid nitrogen or in a cryo-stream at 100K. Crystals can be frozen directly from their growth drops, avoiding the additional step of pre-equilibration with an artificial cryo-condition that can damage the crystal. Eleven different cryocrystallants and sparing use of glycerol ensures a broad sampling of possible cryo conditions. Choose from Wizard Cryo 1 or 2 formulations in tubes or Wizard Cryo 1 and 2 together in a 96-well matrix block.
图片

Hampton 24孔坐滴板 Cryschem Plate

Hampton 24孔坐滴板 Cryschem Plate
Applications
 Sitting drop crystallization
  Heavy atom soaks
  Seeding

Features
Optically clear, concave sitting drop post
Cryschem Plate – Larger footprint and round reagent reservoir
Cryschem M – Microplate footprint with round reagent reservoir
Cryschem S – Microplate footprint with square reagent reservoir
Compatible with Formulatrix Formulator
Smooth, flat top for easy seal
Each plate supplied with a cover
Materials
  Optically clear polystyrene
 Description

The Cryschem Plate is a 24 well sitting drop plate that is sealed with clear sealing tape. The polystyrene plate is supplied with a raised cover so the plate can also be sealed with vacuum grease and plain glass or plastic cover slides (22 mm diameter). The stackable, 24 well plates have distortion-free, flat bottom wells and a concave sitting drop post in the center of the well for concentric equilibration of the drop with the reagent. Each plate is individually sealed. The top of the plate and each of the 24 wells is a single, flat plane for smooth and easy seal application. Well spacing provides for a large seal area between wells to prevent cross contamination and evaporation.
上海金畔生物作为Hampton蛋白结晶产品的代理商,竭诚为您服务,欢迎新老客户咨询。
Cryschem Plate is large plate footprint with circular reagent reservoir. Cryschem plate approximate size: 15.1 cm x 10.6 cm x 2.2 cm. Approximate well size: 1.6 cm x 1.5 cm. Typical fill volume: 500 to 1,000 µl. Well capacity: 1.5 ml. Maximum drop volume on post: 40 µl. Recommended seal: HR3-511 Crystal Clear Sealing Tape (1.88 inch x 43.7 yard roll, with cutter) or HR4-511 Crystal Clear Sealing Tape (1.88 inch x 60 yard roll, without cutter).
Cryschem M Plate is microplate footprint with circular reagent reservoir. Cryschem M microplate approximate size: 128 mm x 86 mm x 14 mm. Approximate circular reservoir size: 15 mm wide x 9 mm deep. Typical reservoir volume: 500 µl. Well capacity: 1.1 ml. Maximum drop volume on post: 30 µl. Recommended seal: HR3-609 Crystal Clear Sealing Film (100 pack), HR4-521 ClearSeal Film (100 pack) or HR4-506 Crystal Clear Sealing Tape (3 inch x 55 yard roll, without cutter). HR4-508 Crystal Clear Sealing Tape (0.75 inch x 650 inch, with cutter) seals a single row at a time.
Cryschem S Plate is microplate footprint with square reagent reservoir. Cryschem S microplate approximate size: 128 mm x 86 mm x 14 mm. Approximate square reservoir size: 15 mm wide x 9 mm deep. Typical reservoir volume: 500 µl. Well capacity: 1.1 ml. Maximum drop volume on post: 30 µl. Recommended seal: HR3-609 Crystal Clear Sealing Film (100 pack), HR4-521 ClearSeal Film (100 pack) or HR4-506 Crystal Clear Sealing Tape (3 inch x 55 yard roll, without cutter). HR4-508 Crystal Clear Sealing Tape (0.75 inch x 650 inch, with cutter) seals a single row at a time.
Cryschem Plate
Cryschem plate

CAT NO NAME DESCRIPTION
HR3-158 Cryschem Plate 24 plate case
HR3-160 Cryschem Plate 100 plate case
HR1-002 Cryschem M Plate (round reagent reservoir) 50 plate case
HR3-308 Cryschem S Plate (square reagent reservoir) 50 plate case

Sigma代理  Abcam代理  CST代理  Santa Cruz代理  Biolegend代理 ebioscience代理  Invitrogen代理   millipore代理  BD流式抗体代理   GeneTe x抗体代理  Novus抗体代理   R&D代理 Biovison代理  Jackson代理  MBL抗体代理  ProSpec抗体代理  Bethyl抗体代理  Antibody Revolution抗体代理  Torrey Pines Biolabs代理  Amresco代理  MPbio代理  Laysan bio代理  NANOCS代理  Avanti代理  wako代理  lumiprobe代理(活性荧光染料)   NEB酶代理  Roche酶代理  Toyobo酶代理   USP代理  EP代理  Dr代理  TRC代理  TCI代理  Reagecon代理 Megazyme代理 Hampton代理(蛋白结晶) whatman代理(滤膜滤纸) GE代理(蛋白纯化) Corning康宁代理  Axygen代理 Falcon代理  NISSUI日水代理 Himedia代理 OXOID代理  BD培养基代理 Ludger代理(糖蛋白分析产品)  Eppendorf代理  Labnet代理  标准品代理 抗体代理 酶试剂代理  培养基代理 耗材代理 Elisa试剂盒代理 。