上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
产品信息
相较于同类型文库定量试剂盒，NEBNext 文库定量试剂盒（Illumina）提供了更简化的操作流程，并减少了批间差。
- 产品类别:
- NGS Library Quantitation Products
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试剂盒组成
本产品提供以下试剂或组分：
NEB # 名称 组分货号 储存温度 数量 浓度 -
E7642S -20 NEBNext® Library Quant DNA Standard 1 E6886AAVIAL -20 1 x 0.12 ml 100 pM NEBNext® Library Quant DNA Standard 2 E6887AAVIAL -20 1 x 0.12 ml 10 pM NEBNext® Library Quant DNA Standard 3 E6888AAVIAL -20 1 x 0.12 ml 1 pM NEBNext® Library Quant DNA Standard 4 E6889AAVIAL -20 1 x 0.12 ml 0.1 pM NEBNext® Library Quant DNA Standard 5 E6890AAVIAL -20 1 x 0.12 ml 0.01 pM NEBNext® Library Quant DNA Standard 6 E6891AAVIAL -20 1 x 0.12 ml 0.001 pM
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优势和特性
应用特性
- NEBNext 文库定量试剂盒（Illumina）包含的同样的六个定量标准品为用户提供了额外的体积。
- 基于四个或六个标准品的 qPCR 法文库定量
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相关产品
相关产品
- NEBNext® 文库定量试剂盒（Illumina®）
操作说明、说明书 & 用法
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操作说明
- Where can I find protocols for using the NEBNext Library Quant DNA Standard (NEB #7642)?
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说明书
产品说明书包含产品使用的详细信息、产品配方和质控分析。- manualE7630_E7642
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应用实例
- Improved library quantitation for a broad range of library types using the NEBNext® Library Quant Kit for Illumina®
FAQs & 问题解决指南
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FAQs
- How are the new 6 standards different from the old 4 standards?
- What are the advantages of the qPCR-based NEBNext Library Quant Kit compared to other methods of quantitating NGS libraries?
- What libraries can be quantitated with the Quant Kit?
- How much should I dilute my library to run in the Quant Kit?
- Can I run the NEBNext Library Quant Kit on my qPCR machine?
- Which ROX should I use for my qPCR machine?
- Are 4 standards enough for a qPCR quantitation?
- Does the library quant require a size correction?
- What other materials/information do I need to run the kit?
- How many libraries can I quantitate at a time?
- How long is a run of the Quant Kit?
- Do you provide any analysis tools to help with the quantitation?
- Can I see adaptor dimers as a measure of library quality?
