Ph.D.™-12 噬菌体展示肽库 |NEB酶试剂 New England Biolabs

发表于由

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

产品信息

Ph.D.-12 噬菌体展示肽库的制备原理是将随机十二肽组合库融合表达于 M13 噬菌体次要外壳蛋白（pIII）（1–6）。展示的肽（12-mer）在 pIII 的 N 端表达，即成熟蛋白的第一残基属于第一个随机位点。这种肽后依次跟有短间隔序列（Gly-Gly-Gly-Ser）和野生型 pIII 序列。该库包含约 10 亿个电穿孔序列，在提供的 10 µl 噬菌体中经扩增一次，每个序列即可产生约 100 个拷贝。

噬菌体展示文献引用

Ph.D.™-12 噬菌体展示肽库 |

对 pIII 上展示的五价肽库进行淘选。

产品类别:
Phage Display Products

应用:
Phage Display

  • 试剂盒组成

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • E8111L     -20    
        Ph.D.™-12 Phage Display Peptide Library E8111LVIAL -20 1 x 0.5 ml 1 x 1013 pfu/ml

  • 相关产品

    相关产品

    • Ph.D.™ 多肽展示克隆系统
    • e4104-ecoli-k12-er2738

  • 参考文献

    1. Devlin, J.J., Panganiban, L.C. and Devlin, P.E. (1990). Science. 249, 404-406.
    2. Smith, G.P. and Scott, J.K. (1993). Methods Enzymol.. 217, 228-257. San Diego: Academic Press.
    3. Cortese et al. (1995). Curr. Opin. Biotechnol.. 6,
    4. Scott, J.K. and Smith, G.P. (1990). Science. 249, 386-390.
    5. Cwirla, S.E., Peters, E.A., Barret, R.W. and Dower, W.J. (1990). Proc. Natl. Acad. Sci. USA. 87, 6378-6382.
    6. Devlin, J.J., Panganiban, L.C. and Devlin, P.E. (1990). Science. 249, 404-406.

操作说明、说明书 & 用法

  • 操作说明

    1. M13 Titer Protocol for Ph.D. Phage Display
    2. M13 Amplifcation Protocol for Ph.D. Phage Display

  • 说明书

    产品说明书包含产品使用的详细信息、产品配方和质控分析。

    • manualE8100_E8101_E8110_E8111_E8120

FAQs & 问题解决指南

  • FAQs

    1. Which of the three ready-made libraries should I choose?
    2. What peptide libraries are available for use with Ph.D.™ Phage Display?
    3. I am using Ph.D.™ Phage display and the amplified phage titer is low.
    4. I am using Ph.D.™ Phage Display and the phage DNA templates do not yield a readable sequence.
    5. I am using Ph.D.™ Phage Display and the sequencing templates do not run where they should on a gel.
    6. I am using Ph.D.™ Phage Display and after 4 or more rounds of panning all clones are wild-type phage (white plaques).
    7. When performing an experiment using Ph.D.™ Phage Display, the ELISA indicates that background binding to the plate is as high as binding to the target.
    8. When using the Ph.D.™ Phage Display, panning yielded a consensus sequence, but no ELISA signal.
    9. I am using Ph.D.™ Phage Display and the streptavidin control experiment did not yield the HPQ consensus sequence.
    10. Can a different bacterial strain be used with the Ph.D.™ Phage Display?
    11. Where can I find references for Ph.D.™ phage display libraries?

  • 问题解决指南

    • Phage Display Troubleshooting Guide