上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
产品信息
产品来源
Factor Xa Protease is purified from bovine plasma and activated by treatment with the activating enzyme from Russell’s viper venom.
- 产品类别:
- Proteases Products
- 应用:
- Fusion Protein Cleavage,
- Target Protein Insolubility ,
- Protein Purification,
- Protein Digestion,
Protein Expression
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产品组分信息
本产品提供以下试剂或组分：
NEB # 名称 组分货号 储存温度 数量 浓度 -
P8010V -20 Factor Xa Protease P8010VVIAL -20 1 x 0.025 ml 1 mg/ml
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P8010S -20 Factor Xa Protease P8010SVIAL -20 1 x 0.05 ml 1 mg/ml
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P8010L -20 Factor Xa Protease P8010LVIAL -20 1 x 0.25 ml 1 mg/ml
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特性和用法
单位定义
1 μg of Factor Xa will cleave 50 μg of MBP fusion protein test substrate, MBP-ΔSal to 95% completion in a total reaction volume of 50 μl in 6 hours or less at 23°C in 20 mM Tris-HCl (pH 8.0 @ 25°C) with 100 mM NaCl and 2 mM CaCl2.
使用浓度
1 mg/ml
去除
Factor Xa will bind specifically to benzamidine-agarose.
分子量
理论上的: 43 kDa
停用
Dansyl-glu-gly-arg-chloromethyl ketone (CALBIOCHEM, #251700) will irreversibly inactivate Factor Xa by covalent attachment at the active site. In a reaction containing 20 μg/ml Factor Xa, 2 μM dansyl-glu-gly-arg-chloromethyl ketone will inactivate >95% of the Factor Xa in 1 minute at room temperature.
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注意事项
- The test substrate MBP-ΔSal is maltose-binding protein fused to a truncated form of paramyosin, with the amino acids Ile-Glu-Gly-Arg at the fusion joint. Greater than 95% of the fusion protein is cleaved in 6 hours or less.
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参考文献
- Nagai, K. et al. (1985). Proc. Natl. Acad. Sci. USA. 82,
- Quinlan, R.A. et al (1989). Cell Sci. 82, 7252-7255.
- Eaton, D. et al (1986). Biochemistry. 25, 505-512.
操作说明、说明书 & 用法
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操作说明
- Cleavage of the Fusion Protein Generated Using The pMAL Protein Fusion and Purification System (E8200)
工具 & 资源
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选择指南
- Protease Selection Chart
- Substrate-based Ligase Selection Chart
