Factor Xa Protease |NEB酶试剂 New England Biolabs

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产品信息

Factor Xa cleaves after the arginine residue in its preferred cleavage site Ile-(Glu or Asp)-Gly-Arg. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate (1,2,3). The most common secondary site, among those that have been sequenced, is Gly-Arg. There seems to be a correlation between proteins that are unstable in E.coli and those that are cleaved by Factor Xa at secondary sites; this may indicate that these proteins are in a partially unfolded state (Walker, I., Riggs, P., unpublished observations). Factor Xa will not cleave a site followed by proline or arginine.

产品来源

Factor Xa Protease is purified from bovine plasma and activated by treatment with the activating enzyme from Russell’s viper venom.

产品类别:
Proteases Products

应用:
Fusion Protein Cleavage,
Target Protein Insolubility ,
Protein Purification,

Protein Digestion,

Protein Expression

  • 产品组分信息

    本产品提供以下试剂或组分:

    NEB # 名称 组分货号 储存温度 数量 浓度
    • P8010V     -20    
        Factor Xa Protease P8010VVIAL -20 1 x 0.025 ml 1 mg/ml
    • P8010S     -20    
        Factor Xa Protease P8010SVIAL -20 1 x 0.05 ml 1 mg/ml
    • P8010L     -20    
        Factor Xa Protease P8010LVIAL -20 1 x 0.25 ml 1 mg/ml

  • 特性和用法

    单位定义

    1 μg of Factor Xa will cleave 50 μg of MBP fusion protein test substrate, MBP-ΔSal to 95% completion in a total reaction volume of 50 μl in 6 hours or less at 23°C in 20 mM Tris-HCl (pH 8.0 @ 25°C) with 100 mM NaCl and 2 mM CaCl2.

    使用浓度

    1 mg/ml

    去除

    Factor Xa will bind specifically to benzamidine-agarose.

    分子量

    理论上的: 43 kDa

    停用

    Dansyl-glu-gly-arg-chloromethyl ketone (CALBIOCHEM, #251700) will irreversibly inactivate Factor Xa by covalent attachment at the active site. In a reaction containing 20 μg/ml Factor Xa, 2 μM dansyl-glu-gly-arg-chloromethyl ketone will inactivate >95% of the Factor Xa in 1 minute at room temperature.

  • 注意事项

    1. The test substrate MBP-ΔSal is maltose-binding protein fused to a truncated form of paramyosin, with the amino acids Ile-Glu-Gly-Arg at the fusion joint. Greater than 95% of the fusion protein is cleaved in 6 hours or less.

  • 参考文献

    1. Nagai, K. et al. (1985). Proc. Natl. Acad. Sci. USA. 82,
    2. Quinlan, R.A. et al (1989). Cell Sci. 82, 7252-7255.
    3. Eaton, D. et al (1986). Biochemistry. 25, 505-512.

操作说明、说明书 & 用法

  • 操作说明

    1. Cleavage of the Fusion Protein Generated Using The pMAL Protein Fusion and Purification System (E8200)

工具 & 资源

  • 选择指南

    • Protease Selection Chart
    • Substrate-based Ligase Selection Chart

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等级 规格 运输包装 零售价(RMB) 库存情况 参考值

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等级 规格 运输包装 零售价(RMB) 库存情况 参考值

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等级 规格 运输包装 零售价(RMB) 库存情况 参考值

082-09321

for Cellbiology 20ug 咨询


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* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动,恕不另行通知,确切详情请联系上海金畔生物科技有限公司。