Bio-Serv动物模型饲料K3583 Safe Harbor

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Bio-Serv动物模型饲料K3583 Safe Harbor

Product Details

An innovative shelter for mice that offers life saving refuge during cage flooding events by providing an opportunity for escape to a higher level where mice can stay warm and dry.

The bi-level design offers a platform for undersized weanlings to reach the food source on cage lids, as well as a means for submissive mice to find shelter and easily move away from dominant mice, thereby minimizing fighting injuries as compared to other shelters that trap the mice inside.

K3583 Safe Harbor

  • Certified (Contaminant Screened)
  • Made of high temp polycarbonate
  • Two levels increase cage complexity
  • A refuge in case of cage flooding
  • Provides shelter and a shelf with many escape openings
  • Enhances both breeding and enrichment programs
    by providing privacy, security and warmth
  • Easy to clean, store and stack
  • Autoclavable and cagewasher safe
Dimensions
2 1/2″ wide x 4 1/2″ long x 2 3/4″ high
Reviews

1

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Ratings Snapshot
5.0
out of 5

5 (1)
4 (0)
3 (0)
2 (0)
1 (0)

Would recommend this product.

100%

Customer Ratings & Reviews

1

Ratings Snapshot
5.0
out of 5

5 (1)
4 (0)
3 (0)
2 (0)
1 (0)

Would recommend this product.

100%

5.0
out of 5

5 (1)
4 (0)
3 (0)
2 (0)
1 (0)

Would recommend this product.

100%

1 Total Reviews

Mice Making Choices

5 out of 5

Jeff Wyatt
Location: University of Rochester
Date: July 27, 2020

We have placed one safe harbor retreat inside each of our 18,000 ventilated, micro-isolator mouse cages. Research and animal care staff enjoy observing the preference choices our mice make huddling inside or on top of the shelters. On the relatively rare occasions we experience cage water supply leaks, our mice climb atop the spacious, flat roof of the retreat to stay dry. We are impressed with the mouse welfare benefits perceived over our first three months since universally employing this simple enrichment strategy.
Recommended

1 Total Reviews

1 kb Plus DNA Ladder for Safe Stains |NEB酶试剂 New England Biolabs

上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。

产品信息

1 kb Plus DNA Ladder for Safe Stains is a pre-mixed, ready-to-load molecular weight marker containing one tracking dye. It has been optimized for use on GelRed®, GelGreen® and SYBR™ Safe™ precast agarose gels.
 
1 kb Plus DNA Ladder for Safe Stains consists of proprietary plasmids, which are digested to completion with appropriate restriction enzymes to yield 19 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 100 bp to 10 kb. The 0.5, 1.0 and 3.0 kb bands have increased intensity to serve as reference bands.

  • Recommended gel percentage range: 1-1.4%
  • Optimum separation on 1.2%

 

1 kb Plus DNA Ladder for Safe Stains |
1 kb Plus DNA Ladder for Safe Stains visualized on a 1.0% TBE agarose gel precast with GelRed.&#x2028 Mass values are for 0.5 µg/lane.&#x2028 Suggested Load: 5 to 10 µl/lane.
产品类别:
DNA Markers & Ladders Products

应用:
DNA Analysis

  • 产品组分信息

    本产品提供以下试剂或组分:

    NEB # 名称 组分货号 储存温度 数量 浓度
    • N0559S     4    
        1 kb Plus DNA Ladder for Safe Stains N0559SVIAL 4 1 x 1.25 ml 50 µg/ml

  • 特性和用法

    碱基

    Fragment Mass (ng) bp
    1 20 10002
    2 20 8001
    3 24 6001
    4 20 5001
    5 16 4001
    6 60 3001
    7 20 2017
    8 28 1517
    9 23 1200
    10 61 1000
    11 17 900
    12 15 800
    13 14 700
    14 11 600
    15 62 500/517
    16 25 400
    17 18 300
    18 16 200
    19 30 100

    有效大小范围

    100bp to 10,002bp

    贮存溶液

    5 mM Tris-HCl
    5 mM EDTA
    5% Glycerol
    0.002% Xylene Cyanol
    pH 8 @ 25°C

  • 相关产品

    相关产品

    • Quick-Load® 紫色 1 Kb Plus DNA Ladder
    • 1 kb Plus DNA Ladder
    • t1010-monarch-plasmid-miniprep-kit
    • Monarch® DNA 胶回收试剂盒
    • Monarch® PCR & DNA 纯化试剂盒(5 μg)

  • 注意事项

    1. DNA 制备:
      双链 DNA 以适当的限制性内切酶完全消化,经苯酚抽提,再在储存缓冲液中平衡。
    2. 用法建议:
      建议在 GelRed® 和 GelGreen® 预制琼脂糖凝胶的每个泳道上上样 5 µl(0.25 µg)的 1 kb Plus DNA Ladder(用于安全染料凝胶)。建议在 SYBR® Safe 预制琼脂糖凝胶的每个泳道上上样 10 µl(0.5 µg)的 1 kb Plus DNA Ladder(用于安全染料凝胶)。
    3. 贮存建议:
      1 kb Plus DNA Ladder(用于安全染料凝胶)可在 25℃ 环境中稳定贮存至少 6 个月。长期贮存时,请贮存在 4℃ 或 -20℃ 环境中。如果贮存在 -20℃ 环境中,请在融化后充分混合。若混合不充分,上样的 DNA Ladder 可能会漂出加样孔。
    4. 用法建议:
      • 1 kb Plus DNA Ladder(用于安全染料凝胶)不适合精确定量 DNA 质量,但是可用于估算强度相当、大小相似的样本中的 DNA 质量。
      • 1 kb Plus DNA Ladder(用于安全染料凝胶)可以在 SYBR Green I 预制琼脂糖凝胶上使用,但可能需要进一步稀释和优化。建议将 SYBR Green I 作为电泳后染料,以得到最佳结果。
      • 建议根据染料厂商建议,仅将 SYBR Gold 作为电泳后染料。
      • 1 kb Plus DNA Ladder(用于安全染料凝胶)不适合用于较短的小型凝胶或快速电泳系统。建议凝胶长度最短为 7 cm,以实现正确有效的分离。迁移电压为 5 V/cm(电极之间的距离),时间为 60 到 90 分钟时,可得到最佳结果。
      • 大部分凝胶染料的运动方向与迁移 DNA 的运动方向相反。因此,预制凝胶底部的染料浓度可能较低,导致底部条带的染色程度不均匀。染料浓度较低的凝胶的长度很大程度上取决于迁移条件(凝胶浓度、电压、缓冲液、电泳时间等)。可向电泳缓冲液中添加一些凝胶染料来抵消这种影响。

  • 参考文献

    1. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Molecular cloning: A Laboratory Manual, (2nd ed.). 10.51-10.67. Cold Spring Harbor: Cold Spring Harbor Laboratory Press.

操作说明、说明书 & 用法

  • 操作说明

    1. Protocol for loading 1 kb Plus DNA Ladder for Safe Stains (NEB #N0559)

工具 & 资源

  • 选择指南

    • DNA Markers & Ladders Selection Tool

FAQs & 问题解决指南

  • FAQs

    1. How much of the 1 kb Plus DNA Ladder for Safe Stains should I load?
    2. Can I use the 1 kb Plus DNA Ladder for Safe Stains with SYBR® Green?
    3. Can I use the 1 kb Plus DNA Ladder for Safe Stains with SYBR® Gold?
    4. Why are the some of the bands in the DNA Ladder fainter at the bottom of the gel?
    5. Can I use the 1 kb Plus DNA Ladder for Safe Stains on mini-gels or fast electrophoresis systems?

DMSO-Safe Acrodisc/25mm/Sterile/0.2um


DMSO-Safe Acrodisc/25mm/Sterile/0.2um

品牌:Pall
CAS No.:
储存条件:室温
纯度:
产品编号

(生产商编号)

等级 规格 运输包装 零售价(RMB) 库存情况 参考值

364-00151

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