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产品信息
产品来源
This preparation is purified from Pichia pastoris containing a clone of the light chain of the bovine enterokinase gene.
- 产品类别:
- Proteases Products
- 应用:
- Fusion Protein Cleavage,
- Protein Purification,
- Protein Digestion
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产品组分信息
本产品提供以下试剂或组分：
NEB # 名称 组分货号 储存温度 数量 浓度 -
P8070S -20 Enterokinase, light chain P8070SVIAL -20 1 x 0.03 ml 16,000 units/ml
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P8070L -20 Enterokinase, light chain P8070LVIAL -20 1 x 0.16 ml 16,000 units/ml
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特性和用法
单位定义
1 unit is defined as the amount of enzyme required to cleave 25 µg of a MBP-EK-paramyosin-ΔSal substrate to 95% completion in 16 hours at 25°C in a total reaction volume of 25 µl.
去除
Enterokinase will bind specifically to trypsin inhibitor agarose (e.g., Sigma T-0637).
贮存溶液
20 mM Tris-HCl
200 mM NaCl
2 mM CaCl2
50% Glycerol
pH 7.2 @ 4°C分子量
实际: 31 kDa
理论上的: 26.3 kDa单位活性检测条件
Two fold dilutions of Enterokinase, light chain are incubated with 25 µg MBP-EK-paramyosin-ΔSal substrate in 20 mM Tris-HCl, 50 mM NaCl, 2 mM CaCl2 (pH 8.0 @ 25°C) in a 25 µl reaction. The reaction mix is incubated at 25°C for 16 hours. Separation of reaction products are visualized by SDS-PAGE.
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相关产品
相关产品
- Furin
- Factor Xa Protease
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注意事项
- NaCl concentration above 250 mM will significantly inhibit enzyme activity.
- Removal: Enterokinase will bind specifically to trypsin inhibitor agarose (e.g., Sigma T-0637).
- Enterokinase is a glycosylated protein.
操作说明、说明书 & 用法
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操作说明
- Protocol for use with Enterokinase, light chain (P8070)
工具 & 资源
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选择指南
- Protease Selection Chart
- Substrate-based Ligase Selection Chart