Hampton 银弹 Silver Bullets

Hampton 银弹 Silver Bullets

Applications

Additive screen for the optimization of protein crystals
For use with soluble proteins and membrane proteins
Additive screen to discover different crystal forms
Secondary or orthogonal crystallization screen when traditional screens are not successful
Additive screen for the optimization of protein solubility and stability
Features

Developed at Hampton Research
Screens a portfolio of small molecules and excipients for their ability to establish stabilizing, intermolecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote lattice formation and crystallization

Small organic acids & organic salts
Biologically active small molecules
Amino acids & peptides
Macromolecular digests
Co-factors & ligands
Biochemical pathway intermediates
Nucleotides, pharmacaphores & carbohydrates

Description
Silver Bullets screens a portfolio of small molecules for their ability to establish stabilizing, inter molecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote stability, lattice formation, and crystallization.1-3
Published results with the Silver Bullets have been very encouraging, with more than twice as many proteins being crystallized overall as were crystallized from controls free of any small molecules.1-3
X-ray diffraction analysis has revealed the small molecule Silver Bullets in the crystal lattice, involved at the centers of hydrogen bonding networks and electrostatic interaction.1-3 Silver Bullets is compatible with hanging, sitting and sandwich drop vapor diffusion, microbatch, free interface, and microdialysis crystallization methods. Silver Bullets can be used with Dynamic Light Scattering (DLS), ThermoFluor, and Size Exclusion Chromatography assays.
Silver Bullets reagent portfolio
•Organic salts and acids
•Biologically active small molecules
•Amino acids and peptides
•Macromolecular digests
The Silver Bullets kit is a library of small molecules that have been shown to promote crystal lattice formation. X-ray diffraction analysis has demonstrated the reagents have the ability to:
•Stabilize the conformation of the protein
•Perturb the interaction of the protein with the solvent
•Participate in forming important lattice contacts
•Build the crystal lattice by forming reversible cross-links between the macromolecules in the crystal
The Silver Bullets kit is composed of 96 solutions in a single Deep Well block (Greiner 786261 block specifications: Total Volume: 0.5 mL Working Volume: 0.03 – 0.7 mL at Room Temperature 0.03 – 0.55 mL at -20ºC, Well Profile: Conical (V), Bottom Well Bottom: Solid, Plate Color: Translucent), HT format.
Each Silver Bullets reagent is a mixture of small molecules or macromolecular digests in 0.02 M HEPES sodium pH 6.8 buffer. Each solution contains between 2 and 20 small molecules.
Silver Bullets reagent volume is 0.5 ml (each well).
ThermoFluor is a registered trademark of Johnson & Johnson.

Hampton  Silver Bullets Bio

Silver Bullets • Silver Bullets Bio
Silver Bullets • Silver Bullets Bio
上海金畔生物作为Hampton蛋白结晶产品的代理商,竭诚为您服务,欢迎新老客户咨询。
Applications 应用

Additive screen for the optimization of protein crystals
For use with soluble proteins and membrane proteins
Additive screen to discover different crystal forms
Secondary or orthogonal crystallization screen when traditional screens are not successful
Additive screen for the optimization of protein solubility and stability

Features

Screens a portfolio of small molecules and excipients for their ability to establish stabilizing, intermolecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote lattice formation and crystallization

Small organic acids & organic salts
Biologically active small molecules
Amino acids & peptides
Macromolecular digests
Co-factors & ligands
Biochemical pathway intermediates
Nucleotides, pharmacaphores & carbohydrates

 

Developed at Hampton Research
Two sets of 96 reagents, composed of more than 1,090 chemicals, of which more than 400 are uniqu

Please note, the Silver Bullets Bio Screen is temporarily unavailable as a new formulation is in development
 

Hampton结晶用缓冲盐试剂盒– SaltRx

Hampton结晶用缓冲盐试剂盒– SaltRx
SaltRx 1 • SaltRx 2 • SaltRx HT

Applications
Primary or secondary, salt and pH matrix crystallization screen for biological macromolecules
Features

Salt versus pH matrix crystallization screen
Samples pH 4 to 9
22 unique salts versus salt concentration and pH
Compatible with microbatch, vapor diffusion, liquid & gel diffusion methods
Tube or Deep Well block format
Preformulated, ready to screen
Developed at Hampton Research
All salts and buffers in screen readily available as Optimize™ reagents for reproducing and optimizing crystals
Individual reagents available through the Hampton Research Custom Shop
 Description
SaltRx was developed by Hampton Research as a primary and secondary, salt and pH based crystallization screen for biological macromolecules. Salt is the only primary crystallization reagent (precipitant) utilized. Based on a design of 96 conditions, the screen evaluates a broad portfolio of crystallization salts of varying concentration and pH. The selection, concentration, and pH of the salts were determined by data mining the BMCD1 and other crystallization databases, crystallization reports in the literature, and internal crystallization trials performed at Hampton Research. Based on this analysis, up to 35% of protein crystallizations involve salt as the primary crystallization reagent. SaltRx can be used as a primary crystallization screen when salt, ionic strength and pH are desired or suspected as appropriate crystallization variables. It is also useful as a secondary screen when salt only reagents/conditions from screens such as Index™, Crystal Screen™, and Grid Screen™ produce crystals and when further screening for additional salt conditions or optimization is desired. Since this screen does not contain volatile organics, it is compatible with microbatch, vapor diffusion, liquid and gel diffusion crystallization methods. SaltRx may also be used for dialysis crystallization in conjunction with Dialysis Buttons™.The original SaltRx kit (HR2-108) contained 96 unique reagents, 10 ml each. This kit has been discontinued and converted into two 48 reagent based kits, SaltRx 1 and SaltRx 2, available separately, which when used together are identical to the original SaltRx.SaltRx 1 contains 48 unique reagents, 10 ml each. SaltRx 1 contains reagents 1-48 of the original SaltRx 96 reagent kit.Salt Rx 2 contains 48 unique reagents, 10 ml each. SaltRx 2 contains reagent 49-96 of the original SaltRx 96 reagent kit.SaltRx HT contains the 96 SaltRx 1 and SaltRx 2 reagents in a single Deep Well block format.Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.
Measured pH range of kit is 4.1 to 9.0 at 25°C
Average measured pH of kit is 6.9 at 25°C
Median measured pH of kit is 7.3 at 25°C
Mode measured pH of kit is 7.4 at 25°C
CAT NO NAME DESCRIPTION
HR2-136 SaltRx HT 1 ml, Deep Well block format
HR2-107 SaltRx 1 10 ml, tube format
HR2-109 SaltRx 2 10 ml, tube format

Hampton 蛋白结晶向导管Wizard Tube

Wizard Tube 
The Wizard™ Classic line of random sparse matrix screens is designed to increase your probability of producing crystals during the coarse screening phase when crystallizing biological macromolecules (proteins, nucleic acids, peptides, and combinations thereof).
The Wizard Classic reagents are proven to be a highly effective starting point in the screening of biological macromolecules. The Wizard Classic formulations include a large range of crystallants, buffers, and salts covering a broad range of crystallization space at pH levels from pH 4.5 to pH 10.5.Choose from Wizard Classic 1, 2, 3, or 4 non-overlapping formulations in matrix blocks or tubes.

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Wizard Cubic LCP
The tools in the Wizard Cubic LCP (lipidic cubic phase) Kit enable researchers to prepare LCP-type crystallizations by hand. Ideal for low-protein experiments: effective protein volume for a single crystallization experiment is about 80 nanoliters. Wizard Cubic LCP Kit tools work especially well when traditional methods have failed to yield crystals. Lipidic cubic phase has worked well for the crystallization of 7TM membrane proteins (proteins with seven transmembrane helices). Four out of six GPCRs (G-protein coupled receptors), an important membrane protein class, and several microbial 7TM proteins have been crystallized using the LCP approach.
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CRYO (I、II)
The Wizard Cryo™ line of random sparse matrix screens is designed for scientists who want to avoid the additional step of optimizing a cryoprotectant condition. Every Wizard Cryo formulation flash-freezes to a clear, amorphous glass in liquid nitrogen or in a cryo-stream at 100K. Crystals can be frozen directly from their growth drops, avoiding the additional step of pre-equilibration with an artificial cryo-condition that can damage the crystal. Eleven different cryocrystallants and sparing use of glycerol ensures a broad sampling of possible cryo conditions. Choose from Wizard Cryo 1 or 2 formulations in tubes or Wizard Cryo 1 and 2 together in a 96-well matrix block.
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Hampton 96孔坐滴蛋白结晶板–Intelli-Plate

Hampton 96孔坐滴蛋白结晶板–Intelli-Plate
在生命科学领域,上海金畔生物为业界提供了丰富的实验室和生物医药生产研发的产品。庆祝上海金畔生物正式成为Hampton Intelli-Plate 96孔蛋白结晶板的签约代理商,在这里要感谢广大客户多年来对上海金畔生物科技有限公司的支持和厚爱,我们将一如既往的为广大客户带来Hampton Intelli-Plate 96孔蛋白结晶板高品质的产品和服务,欢迎广大新老客户来电咨询。
Intelli-Plate 96 (Art Robbins Instruments)
Applications
  Sitting Drop Crystallization   用于蛋白坐滴结晶
Features  特征:

SBS microplate footprint & standard well spacing
Optically clear wells provide for superior crystal imaging
Well layout makes crystal harvesting easier
UV transmissible and low birefringence
2 well versions available in small and large drop well or two identical shallow drop wells
3 well versions are ideal for optimizing protein concentration, additive screens, drop ratio and combinatorial experiments
Rounded reservoir corners to prevent reservoir (MPD) creep

Description
Art Robbins Instruments has developed the ideal family of plates for crystallography applications: the Intelli-Plate. All versions of the Intelli-Plate have the SBS microplate footprint and standard well spacing and are ideal for manual or automated processing.
The Intelli-Plate 96-2 Low Profile (Art Robbins 102-0001-10 Hampton Research HR3-116 and HR3-117) is a low profile version of the Intelli-Plate 96-2 LVR (Low Volume Reservoir). Reservoir volume: 100 µl max. Drop well volume: 4 µl and 10 µl max. UV transmissible and low birefringence.
The CrystalMation Intelli-Plate 96-3 low-profile crystallization plate (Art Robbins 102-0001-13 Hampton Research HR3-118 and HR3-119) is a low profile version of the Intelli-Plate 96-3 LVR (Low Volume Reservoir). The plate is designed for sitting drop vapor diffusion crystallization experiments. It is constructed from optically clear, UV-transmissible, chemically resistant plastic with superior low birefringence. The low-profile construction allows for higher density in plate storage and imaging systems, maximizing your investment and valuable space. The plate is designed to be a substantial improvement in existing low-profile plates, allowing for superior sealing due to thicker well design and a flat sealing surface. It has been built to the SBS (Society for Biomolecular Screening) standard dimensions with 8 vertical wells along the left side of the plate (A-H) and 12 horizontal wells along the top of the plate (1-12). It is compatible with automated instrumentation including the CrystalMation line of instruments from Rigaku. The plate features three identical sized sample drop wells per reservoir. The wells are concave depressions along the left side (Y-axis) of the plate and are located on the ledge above the adjacent, flat bottom reagent reservoir. Each well features a round bottom for easy crystal harvesting and can hold up to 1 µl of sample. The multiple well design allows for combinatorial experiments, complex co-crystallization experiments for ligand screening, or for drop concentration variation. The reagent reservoir is typically filled with 50 to 70 µl of reagent. Reservoir volume: 100 µl max. Drop well volume: 1 µl max. UV transmissible and low birefringence.
The Intelli-Plate 96-2 LVR (Art Robbins 102-0001-00 Hampton Research HR3-143 and HR3-145) is a Low Volume Reservoir version of the original Intelli-Plate. Reservoir volume: 100 µl max. Drop well volume: 1 µl max. UV transmissible and low birefringence.
The Intelli-Plate 96-3 LVR (Art Robbins 102-0001-03 Hampton Research HR3-183 and HR3-185) is a Low Volume Reservoir, 3 drop well version of the Intelli-Plate. Reservoir volume: 100 µl max. Typical reservoir volume 50 µl. Drop well volume: 1 µl max. UV transmissible and low birefringence.
The Intelli-Plate 96-2 Original crystallization plate (Art Robbins 102-0011-00 Hampton Research HR3-297 and HR3-299) is an optically clear plate for sitting drop vapor diffusion crystallization. All wells have standard 9 mm spacing to conform to SBS standards with 8 vertical wells along the left side of the plate (A-H) and 12 horizontal wells along the top of the plate (1-12). This plate features two locations for sample per reservoir. The sample drop locations are located along the left side of the reagent reservoir, along the Y-axis of the plate. The sample wells are concave depressions on the ledge above the adjacent, flat bottom reagent reservoir. One sample well is located above the second sample well. The top sample well can hold 10 µl or less of sample. The lower sample well can hold 4 µl or less of sample. The reagent reservoir is typically filled with 100 µl of reagent and is capable of holding up to 300 µl of reagent. The sidewalls separating adjacent wells or reservoirs are 0.9 and 1.0 mm thick in order to offer a larger area for sealing the plate and a separation of the reservoirs. This plate is rigid, with virtually no torsional flex and is designed for either manual or automated pipetting. UV transmissible and low birefringence.
The Intelli-Plate Flat Shelf (Art Robbins 102-0001-01 Hampton Research HR8-171 and HR8-172) crystallization plate is a flat drop shelf version of the original Intelli-Plate. Reservoir volume: 300 µl max. No drop well. UV transmissible and low birefringence.
The Intelli-Plate 96-2 Shallow Well (Art Robbins 102-0001-20 Hampton Research HR3-163 and HR3-164) has two identically sized 2 µL reaction wells and a 140 µL screen reservoir. Each of the two identical well features a round bottom shallow well design for easy crystal harvesting and is arranged in an 8 x 12 array. This plate has low birefringence and is UV compatible. This plate is also available in a low profile height (Art Robbins 102-0001-21).
The plates can be sealed using Crystal Clear Sealing Film (HR3-609), 3 inch wide Crystal Clear Sealing Tape (HR4-506) or ClearSeal Film™ (HR4-521).
The height of Intelli-Plate is 0.560 inches. The height of Intelli-Plate Low Profile is 0.315 inches (8.001 mm).
Intelli-Plate 96 (Art Robbins Instruments)
Intelli-Plate 96-3

CAT NO NAME DESCRIPTION
HR3-116 Intelli-Plate 96-2 Low Profile 20 plate case
HR3-117 Intelli-Plate 96-2 Low Profile 80 plate case
HR3-118 CrystalMation Intelli-Plate 96-3 low-profile 20 plate case
HR3-119 CrystalMation Intelli-Plate 96-3 low-profile 80 plate case
HR3-143 Intelli-Plate 96-2 LVR 10 plate case
HR3-145 Intelli-Plate 96-2 LVR 40 plate case
HR3-183 Intelli-Plate 96-3 LVR 10 plate case
HR3-185 Intelli-Plate 96-3 LVR 40 plate case
HR3-297 Intelli-Plate 96-2 Original 10 plate case
HR3-299 Intelli-Plate 96-2 Original 40 plate case
HR8-171 Intelli-Plate Flat Shelf 10 plate case
HR8-172 Intelli-Plate Flat Shelf 40 plate case
HR3-163 Intelli-Plate 96-2 Shallow Well 10 plate case
HR3-164 Intelli-Plate 96-2 Shallow Well 40 plate case
HR3-181 Intelli-Plate 96-2 Shallow Well Low Profile 20 plate case
HR3-182 Intelli-Plate 96-2 Shallow Well Low Profile 80 plate case

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