标签归档：methyltransferase

GpC Methyltransferase (M.CviPI) |NEB酶试剂 New England Biolabs

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上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

产品信息

产品来源

The GpC Methyltransferase, M.CviPI, is isolated from a strain of E. coli which contains the methyltransferase gene from Chlorella virus. This construct is fused to the maltose binding protein (MBP).

产品类别:
Methyltransferases for Epigenetics Products,
Methyltransferases Products

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • M0227S     -20    
        GpC Methyltransferase (M.CviPI) M0227SVIAL -20 1 x 0.05 ml 4,000 units/ml
        GC Reaction Buffer B0227SVIAL -20 1 x 1.5 ml 10 X
        S-adenosylmethionine (SAM) B9003SVIAL -20 1 x 0.1 ml 32 mM
    • M0227L     -20    
        GpC Methyltransferase (M.CviPI) M0227LVIAL -20 1 x 0.25 ml 4,000 units/ml
        GC Reaction Buffer B0227SVIAL -20 1 x 1.5 ml 10 X
        S-adenosylmethionine (SAM) B9003SVIAL -20 1 x 0.1 ml 32 mM

  • 特性和用法

    单位定义

    One unit is defined as the amount of enzyme required to protect 1 µg of λ DNA in a total reaction volume of 20 µl in 1 hour at 37°C against cleavage by HaeIII restriction endonuclease. 

    反应条件

    1X GC Reaction Buffer
    Supplement with 160 µM S-腺苷甲硫氨酸（SAM）
    Incubate at 37°C

    1X GC Reaction Buffer
    50 mM NaCl
    50 mM Tris-HCl
    10 mM DTT
    (pH 8.5 @ 25°C)

    对照保护实验

    M.CviPI is incubated with 1 µg λ DNA in 20 µl 1X GC Reaction Buffer and 160 µM S-adenosylmethionine, for one hour at 37°C. The extent of protection by M.CviPI is determined by the addition of 30 µl NEBuffer 2 containing 10 units of HaeIII restriction endonuclease. Incubation for 1 hour at 37°C is followed by analysis on an agarose gel.

    贮存溶液

    15 mM Tris-HCl
    0.2 M NaCl
    1 mM DTT
    0.1 mM EDTA
    200 µg/ml BSA
    50% Glycerol
    pH 7.4 @ 25°C

    热失活

    65°C for 20 minutes

  • 优势和特性

    应用特性

    • Blocking restriction endonuclease cleavage
    • Altering the physical properties of DNA
    • Uniform [3H]-labeling of DNA

  • 相关产品

    单独销售的组分

    • S-腺苷甲硫氨酸（SAM）

  • 注意事项

      • S-腺苷甲硫氨酸（SAM）以 32 mM 浓度溶解于 0.005 M 硫酸和 10% 乙醇溶液中提供。这些条件下，SAM 可在 -20℃ 稳定储存长达六个月的时间。
      • SAM 在 37℃，pH 7.5 的条件下不稳定；因此，当反应温育时间超过 4 小时，需补充 SAM。
    2. 需要新鲜配置的 DTT 来实现最佳活性。为获得最佳结果，每次使用前新配制检测缓冲液。
    3. 胞嘧啶残基的甲基化影响 DNA 的物理特性，包括降低 Z-DNA 形成的自由能（1）、增加 DNA 的螺旋程度（2）、以及改变十字形突出的动力学特性（3）。在化学测序过程（4），由于对联氨的反应活性降低，5-甲基胞嘧啶的位置可被识别。
    4. MgCl2 is not required as a cofactor.

  • 参考文献

    1. Zacharias, W. et al. (1988). Biochemistry. 27, 2970-2978.
    2. Gruenbaum, Y. et al. (1982). Nature. 295, 620-621.
    3. Murchie, A.I. and Lilley, D.M. (1989). J. Mol. Biol.. 205, 593-602.
    4. Ohmori, H. et al. (1978). Nucl. Acids. Res. 5, 1479-1485.
    5. Xu, S. et al. (1998). Nucl. Acids Res.. 26, 3961-3966.
    6. Kladde, M.P. et al. (1991). Methods Enzymol. . 304, 431-447.

操作说明、说明书 & 用法

  • 操作说明

    1. Recommended Protocol for 3H labeling of DNA
    2. Recommended Protocol for Methylation of Genomic DNA

  • 使用指南

    • Activity of DNA Modifying Enzymes in rCutSmart™ Buffer

FAQs & 问题解决指南

  • FAQs

    1. Will GpC Methyltransferase (M. CviPI) work in rCutSmart buffer?
    2. Will any NEB methyltransferases (methylases) work on RNA?

  • 实验技巧

    Make sure the reaction buffer is diluted out just before doing the methylation reaction. This enzyme requires fresh DTT for optimal activity.

HaeIII Methyltransferase |NEB酶试剂 New England Biolabs

发表于由

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

产品信息

产品来源

An E. coli strain that carries the cloned HaeIII modification gene from Haemophilus aegyptius (ATCC 11116).

产品类别:
Methyltransferases for Epigenetics Products,
Methyltransferases Products

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • M0224S     -20    
        HaeIII Methyltransferase M0224SVIAL -20 1 x 0.05 ml 10,000 units/ml
        HaeIII Methyltransferase Reaction Buffer B0224SVIAL -20 1 x 1.5 ml 10 X
        S-adenosylmethionine (SAM) B9003SVIAL -20 1 x 0.1 ml 32 mM

  • 特性和用法

    单位定义

    One unit is defined as the amount of enzyme required to protect 1 µg λ DNA in 1 hour at 37°C in a total reaction volume of 10 µl against cleavage by HaeIII restriction endonuclease.

    反应条件

    1X HaeIII Methyltransferase Reaction Buffer
    Supplement with 80 µM S-腺苷甲硫氨酸（SAM）
    Incubate at 37°C

    1X HaeIII Methyltransferase Reaction Buffer
    50 mM Tris-HCl
    50 mM NaCl
    10 mM DTT
    (pH 8.5 @ 25°C)

    对照保护实验

    HaeIII Methyltransferase is incubated with 1 µg of λ DNA in 10 µl of 1X HaeIII Methyltransferase Reaction Buffer, supplemented with 80 µM S-adenosylmethionine, for one hour at 37°C followed by 15 minutes at 65°C. The extent of protection is determined by the addition of 40 µl NEBuffer 2.1 and 10 units of HaeIII restriction endonuclease. Incubation for 1 hour at 37°C is followed by analysis on an agarose gel.

    贮存溶液

    50 mM Tris-HCl
    1 mM DTT
    10 mM EDTA
    200 µg/ml BSA
    50% Glycerol
    50 mM KCl
    pH 7.4 @ 25°C

    热失活

    65°C for 20 minutes

  • 相关产品

    单独销售的组分

    • S-腺苷甲硫氨酸（SAM）

  • 注意事项

    1. HaeIII 甲基转移酶能保护 DNA 不被 NotI 切割。

  • 参考文献

    1. Hoffman, J.L. (1986). Biochemistry. 25, 4444-4449.

操作说明、说明书 & 用法

  • 操作说明

    1. Recommended Protocol for 3H labeling of DNA
    2. Recommended Protocol for Methylation of Genomic DNA

FAQs & 问题解决指南

  • FAQs

    1. Is S-adenosylmethionine (SAM) supplied with the Methyltransferase?
    2. What should be considered if the methylation is not going to completion?
    3. Does HaeIII Methyltransferase require DTT?
    4. Can HaeIII Methyltransferase be heat inactivated?
    5. Does HaeIII Methyltransferase require MgCl2?
    6. What is the molecular weight of HaeIII Methyltransferase?
    7. Will any NEB methyltransferases (methylases) work on RNA?

HpaII Methyltransferase |NEB酶试剂 New England Biolabs

发表于由

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

产品信息

产品来源

An E. coli strain that carries the cloned HpaII modification gene from Haemophilus parainfluenzae (ATCC 49669).

产品类别:
Methyltransferases for Epigenetics Products,
Methyltransferases Products

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • M0214S     -20    
        HpaII Methyltransferase M0214SVIAL -20 1 x 0.025 ml 4,000 units/ml
        rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X
        S-adenosylmethionine (SAM) B9003SVIAL -20 1 x 0.1 ml 32 mM

  • 特性和用法

    单位定义

    One unit is defined as the amount of enzyme required to protect 1 μg λ DNA in 1 hour at 37°C in a total reaction volume of 10 μl against cleavage by HpaII restriction endonuclease.

    反应条件

    1X rCutSmart™ Buffer
    Supplement with 80 µM S-腺苷甲硫氨酸（SAM）
    Incubate at 37°C

    1X rCutSmart™ Buffer
    50 mM Potassium Acetate
    20 mM Tris-acetate
    10 mM Magnesium Acetate
    100 µg/ml Recombinant Albumin
    (pH 7.9 @ 25°C)

    对照保护实验

    HpaII Methyltransferase is incubated with 1 µg of λ DNA in 10 µl 1X rCutSmart Buffer, supplemented with 80 µM S-adenosylmethionine, for one hour at 37°C followed by 15 minutes at 65°C. The extent of protection by HpaII Methyltransferase is determined by the addition of 40 µl NEBuffer 1 supplemented with 10 mM MgCl2 and 10 units of HpaII restriction endonuclease. Incubation at 37°C for 30 minutes is followed by analysis on agarose gels.

    贮存溶液

    50 mM Tris-HCl
    150 mM NaCl
    0.1 mM EDTA
    5 mM TCEP
    200 µg/ml BSA
    50% Glycerol
    pH 7.5 @ 25°C

    热失活

    65°C for 20 minutes

    分子量

    理论上的: 40397 daltons

  • 相关产品

    单独销售的组分

    • S-腺苷甲硫氨酸（SAM）

  • 参考文献

    1. Hoffman, J.L. (1986). Biochemistry. 25, 4444-4449.

操作说明、说明书 & 用法

  • 操作说明

    1. Recommended Protocol for 3H labeling of DNA
    2. Recommended Protocol for Methylation of Genomic DNA

FAQs & 问题解决指南

  • FAQs

    1. Is S-adenosylmethionine (SAM) supplied with the Methyltransferase?
    2. What should be considered if the methylation is not going to completion?
    3. Can HpaII Methyltransferase be heat inactivated?
    4. Does HpaII Methyltransferase require MgCl2?
    5. What is the molecular weight of HpaII Methyltransferase?
    6. Can HpaII methylated DNA be used to transform E. coli?
    7. Will any NEB methyltransferases (methylases) work on RNA?
    8. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

DNA (cytosine-5) methyltransferase 1 (Dnmt1), Active

发表于由

DNA (cytosine-5) methyltransferase 1 (Dnmt1), Active

品牌：Bio Academia
CAS No.：
储存条件：-20℃
纯度：–
产品编号

(生产商编号)

 等级 规格 运输包装 零售价(RMB) 库存情况 参考值

10-201

 – 300 units – 咨询 – –


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* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。