半乳糖凝集素(稳定型/全长),基因重组体,溶液

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半乳糖凝集素(稳定型/全长),基因重组体,溶液

半乳糖凝集素家族

半乳糖凝集素(稳定型/全长),基因重组体,溶液

半乳糖凝集素家族是能够识别β-半乳糖苷结构的动物外源凝集素的总称,有能够识别高度保存的糖的结构域。根据结构特点可分为原型、手性型和重复型,与外源凝集素活性、细胞增殖、细胞坏死、细胞粘着和游走等生物活性有关,还有可能还与一些疾病有关。



 ◆Gal1 稳定型


原型半乳糖凝集素

结构上的特点:受到氧化的半胱氨酸置换成丝氨酸的半乳糖凝集素1

识别糖:Gal、Branched LacNAc

生理活性:诱导活性化T细胞的细胞凋亡、细胞增殖(氧化型Gal1:促进神经再生)



Gal3(全长)


手性型半乳糖凝集素

结构上特点:C末端有识别糖的领域,有有助于N末端自我凝集的短链重复序列的全长型半乳糖凝集素3

识别糖:polyLacNAc

生理活性:诱导T细胞的细胞凋亡、细胞粘着、巨噬细胞的游走

 


Gal9 稳定性


重复型半乳糖凝集素

结构上特点:在连接2个识别糖的领域(C,N末端侧)添加氨基酸酰胺提高了稳定性的半乳糖凝集素9.

识别糖:poly LacNAc、GalNAcα1-3Gal

生理活性:诱导活性T细胞的细胞凋亡、嗜中性粒细胞游走、癌的转移、树状细胞的成熟化

 


相关产品


产品编号

产品名称

规格

包装

072-06341

Gal1-S Lectin, recombinant, Solution

糖链研究用

1 mg

078-06343

1 mg×5

073-06371

Gal2 Lectin, recombinant, Solution

糖链研究用

1 mg

079-06351

Gal3C-S Lectin, recombinant, Solution

糖链研究用

1 mg

075-06353

1 mg×5

076-06361

Gal7-S Lectin, recombinant, Solution

糖链研究用

1 mg

072-06363

1 mg×5

参考文献


[1] Nishi, N. et al. : FEBS Lett., 579, 2058 (2005).

产品编号 产品名称 产品规格 产品等级
070-06401 Gal1, Stable Form
 半乳凝素1
50 μg 糖链研究用
077-06411 Gal3(Full-length) 
半乳凝素3(全长)
50 μg 糖链研究用
074-06421 Gal9, Stable Form
 半乳凝素9
50 μg 糖链研究用

乳糖/D-半乳糖[快速]检测试剂盒 Lactose/Sucrose/D-Glucose Assay Kit 货号:K-LACGAR Megazyme中文站

乳糖/D-半乳糖[快速]检测试剂盒

Megazyme低聚半乳糖和半乳糖检测试剂盒采用专利技术,在试剂盒中加入半乳糖变旋酶,可快速催化限速变旋步骤,室温下5分钟即可获得检测结果。试剂盒规格:115次方法:分光光度计,340nm 反应时间: 5分钟检测限: 2.96mg/L 样品类型:牛奶、乳制品(如奶油、奶粉、乳清粉、奶酪、炼乳和酸奶)、含乳食品(如保健食品、焙烤食品、婴幼儿食品、巧克力、糖果和冰淇淋)、食品添加剂、饲料、化妆品、医药及其他物料。方法认证: 通过AOAC、NBN、DIN、GOST以及德国、荷兰、瑞士和奥地利的认证.

分析物意义:常见加工食品组分,在某些情况下,精确的数值很重要,如 “无乳糖”产品 

Megazyme检测试剂盒优点:K-LACGAR试剂盒反应快(室温,5min)、试剂稳定

The Lactose/Galactose (Rapid) test kit is used for the rapid test of lactose, D-galactose and L-arabinose in food and plant products. Galactose dehydrogenase can be used the measurement and analysis of both D-galactose and L-arabinose. Suitable for the analysis of lactose in “low-lactose” or “lactose-free” samples which contain high levels of monosaccharides.

UV-method for the determination of Lactose and D-Galactose in
foodstuffs, beverages and other materials

Principle:
(β-galactosidase)
(1) Lactose + H2O → β-D-galactose + D-glucose

(galactose mutarotase)
(2) α-D-Galactose ↔ β-D-galactose

(β-galactose dehydrogenase)
(3) β-D-Galactose + NAD+ → D-galactonic acid + NADH + H+
 

Kit size: 115 assays
Method: Spectrophotometric at 340 nm
Reaction time: ~ 15 min
Detection limit: 2.96 mg/L (lactose)
Application examples:
Milk, dairy products (e.g. cream, milk / whey powder, cheese,
condensed milk and yogurt), foods containing milk (e.g. dietetic foods,
bakery products, baby food, chocolate, sweets and ice-cream), food
additives, feed, cosmetics, pharmaceuticals and other materials
(e.g. biological cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by AOAC, NBN,
DIN, GOST and IDF

Advantages

  • Very rapid reaction due to inclusion of galactose mutarotase (patented technology PCT / IE2004 / 00170)
  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included