Monarch® RNA Cleanup Columns (10 μg) |NEB酶试剂 New England Biolabs

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产品信息

The Monarch RNA Cleanup Columns (10 µg) can be used to purify up to 10 µg of nucleic acid from enzymatic reactions. The columns are designed without the use of a retaining ring, helping to ensure no buffer retention and no carryover of contaminants. Nucleic acid can be eluted in as little as 6 μl and is ready for use in a variety of downstream applications including RT-PCR, RNA library prep for NGS, and labelling.

Monarch RNA Cleanup Columns are also available for 50 µg (NEB #T2047) and 500 µg (NEB #T2057) binding capacities.

Properties

Also included as a component in: T2030 Monarch RNA Cleanup Kit (10 µg)
Product format: Spin columns and collection tubes for nucleic acid purification
Technology and purification format: Silica membrane-based purification technology
Provided as: 100 columns and 100 collection tubes
Suitable for: Cleaning up nucleic acid from a variety of enzymatic reactions; Concentration of nucleic acid into small volumes

 

产品类别:
RNA Cleanup Products,
RNA Extraction and Purification,
Nucleic Acid Purification Products

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • T2037L     25    
        Monarch® RNA Cleanup Columns (10 μg) T2037-1 25 2 x 50 columns Not Applicable
        Monarch® Collection Tubes II T2018-1 25 2 x 50 tubes Not Applicable

操作说明、说明书 & 用法

  • 使用指南

    • Avoiding Ribonuclease Contamination
    • Guidelines for RNA Quantitation
    • Guidelines for Working with RNA During RNA Cleanup

  • 应用实例

    • A Practical Guide to Analyzing Nucleic Acid Concentration and Purity with Microvolume Spectrophotometers

FAQs & 问题解决指南

  • FAQs

    1. What is the maximum binding capacity of the Monarch RNA Cleanup Column provided with the Monarch RNA Cleanup Kit?
    2. What is the smallest volume of nuclease-free water that can be used for elution with the Monarch RNA Cleanup Columns?
    3. Can I get better recovery with the Monarch RNA Cleanup Kits if I do a second elution with my eluent from the first elution?
    4. What factors affect my (A260/A230) when using the Monarch RNA Cleanup Kits?
    5. What size RNA can be purified with the Monarch RNA Cleanup Kit?
    6. Can I use the Monarch RNA Cleanup Kit to cleanup up my DNase I-treated RNA?
    7. Can I use the Monarch RNA Cleanup Kits to purify RNA from agarose gels?
    8. Can I use the Monarch RNA Cleanup Kits to cleanup RNA after a TRIzol®/chloroform extraction?
    9. Are the Monarch RNA Cleanup Kits (NEB # T2030, #T2040, #T2050) compatible with Luna RT-qPCR reagents?
    10. Are the Monarch RNA Cleanup Kits (NEB #T2030, #T2040, #T2050) compatible with NEBNext reagents for RNA library prep?
    11. How can I assess RNA integrity and purity?
    12. Are the columns in the Monarch RNA Cleanup Kits the same as those in the Monarch Total RNA Miniprep Kit (NEB #T2010)?
    13. Can I purchase Monarch buffers and columns separately?
    14. Can I do an on-column DNase I treatment with the Monarch RNA Cleanup Columns?
    15. Can the Monarch RNA Cleanup Kits (NEB #T2030, #T2040, #T2050) also be used to purify DNA?
    16. Can the Monarch RNA Cleanup Kits be used for RNA extraction?

  • 问题解决指南

    • Troubleshooting Guide for RNA Cleanup