Monarch® RNA 提取离心柱 |NEB酶试剂 New England Biolabs

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产品信息

Monarch® RNA 提取离心柱 |

Monarch® RNA 提取离心柱随 Monarch 总 RNA 小量提取试剂盒提供，可从多种起始样本中提取多达 100 µg 的总 RNA。离心柱随附 Monarch 收集管 II，并且与真空歧管兼容。

Monarch® RNA 提取离心柱 |

产品类别:
Total RNA Extraction & Purification Products,
RNA Extraction and Purification,
Nucleic Acid Purification Products

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • T2007L     25    
        Monarch® RNA Purification Columns T2007-1 25 2 x 50 columns Not Applicable
        Monarch® Spin Collection Tubes T2118-1 25 2 x 50 tubes Not Applicable

  • 相关产品

    相关产品

    • Luna® 通用一步法 RT-qPCR 试剂盒
    • Luna® 通用探针一步法 RT-qPCR 试剂盒
    • e6560-protoscript-ii-first-strand-cdna-synthesis-kit
    • NEBNext® Ultra™ II RNA 非链特异性文库制备试剂盒
    • e7760-nebnext-ultra-ii-directional-rna-library-prep-kit-for-illumina
    • e7765-nebnext-ultra-ii-directional-rna-library-prep-with-sample-purification-beads
    • NEBNext® rRNA 去除试剂盒 – 人/小鼠/大鼠（含 RNA 纯化磁珠）
    • e7490-nebnext-polya-mrna-magnetic-isolation-module
    • m0368-protoscript-ii-reverse-transcriptase
    • t1010-monarch-plasmid-miniprep-kit
    • Monarch® DNA 胶回收试剂盒
    • Monarch® PCR & DNA 纯化试剂盒（5 μg）

    单独销售的组分

    • Monarch® 总 RNA 小量提取试剂盒
    • Monarch® DNA/RNA 保护试剂
    • Monarch® gDNA 去除离心柱
    • Monarch® 收集管 II
    • Monarch® RNA 裂解液
    • Monarch® RNA 初始洗涤缓冲液
    • t2014-monarch-rna-wash-buffer
    • Monarch® 总 RNA 小量提取酶套装
    • Nuclease-free Water

操作说明、说明书 & 用法

  • 操作说明

    1. Download Quick Protocol Card T2010
    2. Quick Protocol for Total RNA Extraction (NEB #T2010)
    3. Total RNA Purification from Samples in RNAlater® using the Monarch Total RNA Miniprep Kit (NEB #T2010)
    4. Total RNA Purification from TRIzol®-extracted Samples using the Monarch Total RNA Miniprep Kit (NEB #T2010)
    5. Total RNA Purification from Samples Stored in Monarch DNA/RNA Protection Reagent using the Monarch Total RNA Miniprep Kit (NEB #T2010)
    6. FFPE Tissue Deparaffinization and Subsequent RNA Purification Using the Monarch Total RNA Miniprep Kit  (NEB #T2010)
    7. In-tube DNase I Treatment after Purification with the Monarch Total RNA Miniprep Kit (NEB #T2010)
    8. Fractionation of Small and Large RNA Using the Monarch Total RNA Miniprep Kit (NEB #T2010)
    9. RNA Reaction Cleanup using the Monarch Total RNA Miniprep Kit (NEB #T2010)
    10. Total RNA Purification from Mammalian Whole Blood Samples using the Monarch Total RNA Miniprep Kit (NEB #T2010)
    11. RNA Extraction from Saliva, Buccal Swabs, and Nasopharyngeal Swabs using the Monarch Total RNA Miniprep Kit (NEB #T2010)
    12. Automation of RNA Extraction from Saliva Using the Monarch Total RNA Miniprep Kit
    13. Total RNA Purification from Cultured Mammalian Cells using the Monarch Total RNA Miniprep Kit (NEB #T2010)
    14. Total RNA Purification from Tissues and Leukocytes using the Monarch Total RNA Miniprep Kit (NEB #T2010)
    15. Total RNA Purification from Tough-to-Lyse Samples (bacteria, yeast, plant, etc.) using the Monarch Total RNA Miniprep Kit (NEB #T2010)

  • 使用指南

    • General Guidelines for Successful RNA Purification Using the Monarch Total RNA Miniprep Kit
    • Guidance on Choosing Sample Input Amounts when Using the Monarch Total RNA Miniprep Kit
    • Guidelines for Purification of RNA from Cultured Mammalian Cells
    • Guidelines for RNA Purification from Bacteria
    • Guidelines for RNA Purification from Leukocytes (WBCs, PBMCs)
    • Guidelines for RNA Purification from Plants
    • Guidelines for RNA Purification from Tissues 
    • Guidelines for RNA Purification from Whole Blood
    • Guidelines for RNA Purification from Yeast
    • Guidelines for Sample Stabilization Using the Monarch DNA/RNA Protection Reagent

  • 应用实例

    • A Practical Guide to Analyzing Nucleic Acid Concentration and Purity with Microvolume Spectrophotometers

FAQs & 问题解决指南

  • FAQs

    1. Is the Monarch Total RNA Miniprep Kit compatible with Luna RT-qPCR Reagents?
    2. Is the Monarch Total RNA Miniprep Kit compatible with NEBNext reagents for RNA Library Prep?
    3. What is the lowest volume in which I can elute my RNA with the Monarch Total RNA Miniprep Kit (NEB #T2010)?
    4. How can I tell the difference between the gDNA Removal Column and the RNA Purification Column?
    5. Can the Monarch Total RNA Miniprep Kit be used to purify RNA from yeast?
    6. Can the Monarch Total RNA Miniprep Kit be used to purify RNA from bacteria?
    7. Can the Monarch Total RNA Miniprep Kit be used to purify RNA from plants?
    8. Can I use the Monarch Total RNA Miniprep Kit for RNA cleanup after enzymatic manipulations?
    9. Can the Monarch Total RNA Miniprep Kit (NEB #T2010) be used to purify RNA from saliva?
    10. Can the Monarch Total RNA Miniprep Kit NEB T2010 be used to purify RNA from buccal cheek swabs?
    11. Can the Monarch Total RNA Miniprep Kit (NEB #T2010) be used to purify RNA from plasma or serum?
    12. Can the Monarch Total RNA Miniprep Kit (NEB #T2010) be used to purify RNA from nucleated blood (e.g. bird, fish, reptile)?
    13. Can the Monarch Total RNA Miniprep Kit be used to purify RNA from drosophila?
    14. Can the Monarch Total RNA Miniprep Kit be used to purify RNA from zebrafish embryos/larvae?

  • 问题解决指南

    • Troubleshooting Guide for Total RNA Extraction & Purification