Monarch® gDNA 洗脱缓冲液 |NEB酶试剂 New England Biolabs

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产品信息

Monarch gDNA 洗脱缓冲液含 10 mM Tris-HCl（pH 9.0）、0.1 mM EDTA，适用于长期贮存基因组 DNA。添加 EDTA 和溶液 pH 可以使核酸酶失活。

产品类别:
Genomic DNA Extraction & Purification Products,
Nucleic Acid Purification Products

应用:
Nucleic Acid Purification

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • T3016L     25    
        Monarch® gDNA Elution Buffer T3016-2 25 1 x 34 ml Not Applicable

操作说明、说明书 & 用法

  • 操作说明

    1. Quick Protocol for Extraction and Purification of Genomic DNA
    2. Protocol for Extraction and Purification of Genomic DNA from Cells (NEB #T3010)
    3. Protocol for Extraction and Purification of Genomic DNA from Blood (NEB #T3010)
    4. Protocol for Extraction and Purification of Genomic DNA from Tissues (NEB #T3010)
    5. Protocol for Genomic DNA Cleanup (NEB #T3010)
    6. Genomic DNA Purification from Gram-positive Bacteria and Archaea (NEB #T3010)
    7. Genomic DNA Purification from Yeast (NEB #T3010)
    8. Genomic DNA Purification from Saliva (NEB #T3010)
    9. Genomic DNA Purification from Buccal Swabs (NEB #T3010)
    10. Genomic DNA Purification from Gram-negative Bacteria (NEB #T3010)

  • 使用指南

    • Choosing Input Amounts for the Monarch Genomic DNA Purification Kit
    • Factors Affecting DNA Quality when Purifying gDNA from Blood and Tissues with the Monarch Genomic DNA Purification Kit
    • Guidelines for Handling Tissue Samples when using the Monarch Genomic DNA Purification Kit

FAQs & 问题解决指南

  • FAQs

    1. What is the composition of each buffer provided with the Monarch® Genomic DNA Purification Kit?
    2. What is the minimum elution volume that can be used with the Monarch® Genomic DNA Purification Kit columns?
    3. How important is it to pre-heat the elution buffer to 60°C? Would 56°C be OK?
    4. Can I get better recovery with the Monarch® Genomic DNA Purification Kit if I do a second elution with my eluent from the first elution?
    5. Can I use a different elution buffer than the one supplied with the Monarch Genomic DNA Purification Kit (NEB #T3010)?
    6. What size gDNA can be purified with the Monarch® Genomic DNA Purification Kit?
       
    7. How can I assess gDNA integrity and purity?
    8. Can I omit the low-speed binding centrifugation step to save time?
    9. How should I store purified gDNA samples?
    10. When working with other commercial silica column-based kits, I occasionally see a white precipitate in the eluate. What is this?
    11. Is Monarch-purified gDNA compatible with Next Generation Sequencing (NGS) platforms like PacBio and nanopore?

  • 问题解决指南

    • Troubleshooting Guide for Genomic DNA Extraction & Purification (NEB #T3010)