免疫动物:小鼠免疫原:人胸腺细胞和T淋巴细胞应用:Flow Cytometry (1µg/ml)、Immunocytochemistry (10µg/ml)Application note: paraformaldehyde fixation can be usedImmunohistochemistry (paraffin sections – no pre-treatment of tissue sections is essential)Immunoprecipitation、Western Blot (1µg/ml); Positive control: JURKAT human leukemia T-cell lysate, Kg-1a human leukemia cell lysate.Sample preparation: buffer with laurylmaltoside, 2x non-reducing SDS.Application note: non-reducing conditions. SDS-PAGE (6% separating gel).Optimal conditions must be determined individually for each application.特异性:Recognizes all isoforms of human CD45