β-N-乙酰己糖胺酶(原核生物) β-N-Acetylhexosaminidase (prokaryote) 货号:E-BNAHP Megazyme中文站

High purity β-N-Acetylhexosaminidase (prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.52
CAZy Family: GH20
CAS: 9012-33-3

beta-N-acetyl-D-hexosaminide; N-acetylhexosaminohydrolase

Recombinant. From a prokaryote.
In 3.2 M ammonium sulphate.

Specific activity: 
~ 260 U/mg protein (on pNP-β-D-N-acetylglucosamine) at pH 4.0 and 40°C; 
~ 723 U/mg protein (on pNP-β-D-N-acetylglucosamine) at pH 4.0 and 60°C.

Stability: > 2 years 4°C

DESCRIPTION

β-N-Acetylhexosaminidase (prokaryote)

EC 3.2.1.52
CAZy Family: GH20
CAS: 9012-33-3

Synonyms:
beta-N-acetyl-D-hexosaminide 
N-acetylhexosaminohydrolase

Form:
In 3.2 M ammonium sulphate.

Stability:
> 2 years at 4oC.

Specific activity:
~ 260 U/mg protein (on pNP-β-D-N-acetylglucosamine) at pH 4.0 and 40oC;
~ 723 U/mg protein (on pNP-β-D-N-acetylglucosamine) at pH 4.0 and 60oC.

Unit definition:
One Unit of pyruvate kinase activity is defined as the amount of enzyme required to convert one µmole of phosphoenolpyruvate to pyruvate per min in Tris.HCl buffer (100 mM) at pH 7.2 at 37oC.

Specificity:
Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-β-D-hexosaminides from glycoproteins and oligosaccharides.

Applications:
For use in glycobiology research.