Megazyme乳果糖检测试剂盒采用酶法分析原理，可定量检测鲜奶、UHT奶、浓缩乳和奶粉等各种乳品中的乳果糖。与农业部新标准采用相同的检测原理和方法该试剂盒采用ISO方法11285：2004，经过改进后，更加快速和灵敏灵敏度是传统己糖激酶法的两倍成本低试剂配制后可稳定保存2年采用试剂盒形式，包含检测必需的所有酶提供计算软件，使数据处理更方便包含标准品
The Lactulose Assay Kit is suitable for the specific, rapid and sensitive measurement and analysis of lactulose in milk and milk-based samples. Reagents included in this kit may also be prepared for use in the procedure described by ISO Method 11285:2004.
UV-method for the determination of Lactulose in milk and
foodstuffs containing dairy products
Principle:
(β-galactosidase)
(1) Lactulose + H2O → D-galactose + D-fructose
(glucose oxidase + catalase + H2O2)
(2) D-Glucose + H2O + O2 → D-gluconic acid + H2O2
(hexokinase)
(3) D-Fructose + ATP → F-6-P + ADP
(phosphoglucose isomerase)
(4) F-6-P → G-6-P
(glucose-6-phosphate dehydrogenase)
(5) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+
(gluconate-6-phosphate dehydrogenase)
(6) Gluconate-6-phosphate + NADP+ → ribulose-5-phosphate + NADPH
+ CO2 + H+
Kit size: 50 assays
Method: Spectrophotometric at 340 nm
Reaction time: ~ 120 min
Detection limit: 4.8 mg/L
Application examples:
Milk, dairy products and foods containing milk
Method recognition: Novel method
Advantages
- Twice the sensitivity of traditional hexokinase based lactulose methods
- Very cost effective
- All reagents stable for > 2 years after preparation
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included