The L-Malic Acid (Analyser Format) test kit is an analyser format for the specific measurement and analysis of L-malic acid (L-malate) in beverages and food products. On calibration, the prepared reagent is linear to > 80 micrograms of L-malic acid per mL of assay solution.

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4^oC.

Analyser format UV-method for the determination of L-Malic Acid
in foodstuffs, beverages and other materials

Principle:
(L-malate dehydrogenase)
(1) L-Malic acid + NAD⁺ ↔ oxaloacetate + NADH + H⁺

(glutamate-oxaloacetate transaminase)
(2) Oxaloacetate + L-glutamate → L-aspartate + 2-oxoglutarate

Kit size:                            245.5 mL of prepared reagent (R1 + R2)
Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 3 min
Detection limit:                 20 mg/L (recommended assay)
Application examples: 
Wine, beer, fruit juices, soft drinks, candies, fruit and vegetables,
bread, cosmetics, pharmaceuticals and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:     
Methods based on this principle have been accepted by AOAC, EEC,
EN, NF, NEN, DIN, GOST, OIV, IFU, AIJN and MEBAK

Advantages

• PVP incorporated to prevent tannin inhibition
  
• Very stable reagent when prepared for auto-analyser applications
  
• Linear calibration (R² ~ 0.9994) up to 80 μg/mL of L-malic acid in final reaction solution
  
• Validated by the University of Wine, Suze la Rousse, France
  
• Very competitive price (cost per mL of reagent)
  
• Both enzymes supplied as stable suspensions
  
• Very rapid reaction (~ 3 min)