Furin |NEB酶试剂 New England Biolabs

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产品信息

Furin is a ubiquitous subtilisin-like proprotein convertase. It is the major processing enzyme of the secretory pathway and is localized in the trans-golgi network (1,2). Substrates of Furin include blood clotting factors, serum proteins and growth factor receptors such as the insulin-like growth factor receptor. The minimal cleavage site is Arg-X-X-Arg’. However, the enzyme prefers the site Arg-X-(Lys/Arg)-Arg’. An additional arginine at the P6 position appears to enhance cleavage (3). Furin is inhibited by EGTA, α1- Antitrypsin Portland (4) and polyarginine compounds (5).

产品来源

Isolated from Spodoptera frugiperda (Sf9) cells infected with recombinant baculovirus carrying truncated human furin.

产品类别:
Proteases Products

应用:
Fusion Protein Cleavage,
Protein Purification,
Protein Digestion

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • P8077S     -20    
        Furin P8077SVIAL -20 1 x 0.025 ml 2,000 units/ml
    • P8077L     -20    
        Furin P8077LVIAL -20 1 x 0.125 ml 2,000 units/ml

  • 特性和用法

    单位定义

    1 unit is defined as the amount of enzyme required to cleave 25 µg of a MBP-FN-paramyosin-ΔSal substrate to 95% completion in 6 hours at 25°C in a total reaction volume of 25 µl.

    贮存溶液

    10 mM MES
    1 mM CaCl2
    50% Glycerol
    pH 7 @ 25°C

    分子量

    理论上的: 52.7 kDa

    单位活性检测条件

    Two fold dilutions of Furin are incubated with 25 µg MBP-FN-paramyosin-ΔSal substrate in 20 mM HEPES, 0.1% Triton X-100, 1 mM CaCl2, 0.2mM β-mercaptoenthanol (pH 7.5 @ 25°C) in a 25 µl reaction. The reaction mix is incubated at 25°C for 6 hours. Separation of reaction products are visualized by SDS-PAGE.

  • 相关产品

    相关产品

    • Enterokinase, light chain
    • Factor Xa Protease

  • 注意事项

    1. Both Furin and Onchocerca volvulus Blisterase will cleave peptide substrates with the sequence, Arg-X- (Lys/Arg)-Arg. However, the ability of either enzyme to cleave a particular protein substrate depends on its tertiary structure as well as on the amino acids immediately surrounding the cleavage site (6).

  • 参考文献

    1. van den Ouweland, A.M.W. et al. (1990). Nucl. Acids Res. 18, 664.
    2. Steiner, D.F. (1998). Curr. Opin. Chem. Biol.. 2,
    3. Krysan, D.J. et al. (1999). J. Biol. Chem.. 274, 23229-23234.
    4. Jean, F. et al. (1998). Proc. Natl. Acad. Sci. USA. 95, 7293-7298.
    5. Cameron, A. et al. (2000). J. Biol. Chem.. 275, 36741-36749.
    6. Poole, C.B. et al. (2003). J. Biol. Chem.. 278, 36183-36190.

工具 & 资源

  • 选择指南

    • Protease Selection Chart
    • Substrate-based Ligase Selection Chart