SNAP-Surface® 649 |NEB酶试剂 New England Biolabs

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产品信息

This package includes 50 nmol of SNAP-Surface 649 substrate, sufficient to make 10 ml of a 5 μM SNAP-tag fusion protein labeling solution.

The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is based on mammalian O6-alkylguanine- DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylguanines and benzylchloropyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

There are two steps to using this system: subcloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAPtag substrate is described below.

Excitation (dotted line) and emission spectra of SNAP-Surface 649 coupled to SNAP-tag in buffer at pH 7.5SNAP-Surface® 649 |
SNAP-Surface® 649 |
Structure of SNAP-Surface 649 (MW 1221.3 g/mol).
产品类别:
SNAP-tag Substrates Products ,
Protein Labeling Products

应用:
Snap Surface Products,
Pulse Chase,
In vivo Imaging,

Receptor Internalization,
Protein Localization,

Protein Labeling Snap Clip

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • S9159S     -20    
        SNAP-Surface® 649 S9159SVIAL -20 1 x 50 nmol Not Applicable

  • 特性和用法

    需要但不提供的材料

    • Cells expressing SNAP-fusion proteins. Proteins of interest can be expressed with the SNAP-tag as either an N- or a C-terminal fusion, but note that the tag needs to be exposed to the extracellular surface of the plasma membrane for labeling with SNAPSurface 649.
    • Tissue culture materials and media
    • Transfection reagents
    • Fluorescence microscope with suitable filter set
    • DMSO

    激发

    655nm

    发射

    676nm

  • 注意事项

    1. Storage: SNAP-Surface 649 should be stored at –20°C (longterm) or at 4°C in the dark (short term, less than4 weeks). Protect the substrate from light and moisture.With proper storage at –20°C the substrateshould be stable for at least three years dry or3 months dissolved in DMSO.

操作说明、说明书 & 用法

  • 操作说明

    1. Cellular Labeling (S9159)
    2. Labeling of Proteins in vitro (S9159)
    3. View the video “Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging” in the Journal of Visualized Experiments (JoVE)

FAQs & 问题解决指南

  • FAQs

    1. Cellular Imaging and Analysis FAQs