BL21(DE3) E. coli 感受态细胞 |NEB酶试剂 New England Biolabs

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产品信息

大肠杆菌（E. coli）化学感受态细胞，适合转化和蛋白表达。

重点

  • T7 表达菌株
  • Lon 和 OmpT 蛋白酶缺失
  • 抗噬菌体 T1（fhuA2）
  • B 菌株
  • 无动物来源产品

基因型

fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS
λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5

产品类别:
T7 Expression,
E. coli Protein Expression Strains Products,
Protein Expression Products,

E. coli Expression Strains Products

应用:
T7 Expression,
Peptide Ligation,
Protein Expression in E. Coli,

Protein Expression

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • C2527H     -80    
        SOC Outgrowth Medium B9020SVIAL 4 1 x 25 ml Not Applicable
        pUC19 Vector N3041AVIAL -20 1 x 0.025 ml 50 pg/µl
        BL21(DE3) Competent E. coli C2527HVIAL -80 20 x 0.05 ml Not Applicable
    • C2527I     -80    
        SOC Outgrowth Medium B9020SVIAL 4 1 x 25 ml Not Applicable
        pUC19 Vector N3041AVIAL -20 1 x 0.025 ml 50 pg/µl
        BL21(DE3) Competent E. coli C2527IVIAL -80 6 x 0.2 ml Not Applicable

  • 特性和用法

    Antibiotic for Plasmid Selection

    用于质粒筛选的抗生素 使用浓度
    Ampicillin 100 µg/ml
    Carbenicillin 100 µg/ml
    Chloramphenicol 33 µg/ml
    Kanamycin 30 µg/ml
    Streptomycin 25 µg/ml
    Tetracycline 15 µg/ml

    货运单

    • Ships on dry ice

  • 优势和特性

    Features

    • 常规 T7 表达

  • 相关产品

    相关产品

    • BL21 E. coli 感受态细胞
    • Lemo21（DE3）E. coli 感受态细胞
    • NiCo21（DE3）E. coli 感受态细胞

    单独销售的组分

    • SOC Outgrowth 培养基

  • 注意事项

    1. 注意：本产品含有害物质 DMSO。处理前请先查看 MSDS。
    2. 贮存和处理：感受态细胞应在 -80℃ 下贮存。在 -20℃ 贮存会导致转化效率显著降低。当细胞温度高于 -80℃ 时，即使不融化，细胞也会失去效率。

操作说明、说明书 & 用法

  • 操作说明

    1. 5 Minute Transformation Protocol (C2527)
    2. Protein Expression Using BL21(DE3) (C2527)
    3. Transformation Protocol for BL21(DE3) Competent Cells (C2527)

  • 使用指南

    • Additional E. coli Strain Genotypes
    • Competent E. coli Genome Sequences Tool
    • Genetic Markers
    • Making Unmethylated (Dam- Dcm-) DNA
    • McrA, McrBC and EcoKI Strain Phenotypes

  • 应用实例

    • Protein Expression with T7 Express Strains

工具 & 资源

  • 选择指南

    • Characteristics of Select E.coli Strains
    • Competent Cell Product Comparison
    • Competent Cell Selection Guide

FAQs & 问题解决指南

  • FAQs

    1. Does New England Biolabs offer strains of Competent E.coli suited for protein expression?
    2. What are the strain properties (C2527)?
    3. Why are there no colonies or no growth in liquid culture (C2527)?
    4. Why is induced protein insoluble (C2527)?
    5. Why is there no protein visible on gel or no activity (C2527)?
    6. What are the solutions/recipes (C2527)?
    7. Can I store competent cells at -20°C instead of -80°C?
    8. Which kind of transformation tubes should be used?
    9. What volume of DNA can be added into competent cells?
    10. What is the shelf life for this strain (NEB #C2527H and NEB #C2527I)?
    11. Are NEB’s competent cells compatible with the “Mix & Go” protocol?
    12. What is the difference between BL21 and BL21(DE3) competent E.coli cells?
    13. How should I store SOC Outgrowth Medium? The SOC I received with my competent cells recommends storage at either room temperature or 4°C, however, when I purchase it as a stand alone product, it recommends storing it at 4°C. Which is better?
    14. Is T7 expression subject to catabolite repression in BL21(DE3) and derivative strains?