Express® Competent E. coli (High Efficiency) |NEB酶试剂 New England Biolabs

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产品信息

重点

  • Enhanced BL21 derivative ideal for Plac, Ptac, Ptrc expression vectors
  • Deficient in proteases Lon and OmpT
  • Resistant to phage T1 (fhuA2)
  • Does not restrict methylated DNA (McrA–, McrBC–, EcoBr–m–, Mrr–)
  • Free of animal products

基因型

fhuA2 [lon] ompT gal sulA11 R(mcr-73::miniTn10–TetS)2 [dcm] R(zgb-210::Tn10–TetS) endA1 Δ(mcrC-mrr)114::IS10

产品类别:
Non-T7 Expression,
E. coli Protein Expression Strains Products,
Protein Expression Products,

E. coli Expression Strains Products

应用:
Non-T7 Expression,
Protein Expression in E. Coli,
Protein Expression

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • C2523H     -80    
        SOC Outgrowth Medium B9020SVIAL 4 1 x 25 ml Not Applicable
        pUC19 Vector N3041AVIAL -20 1 x 0.025 ml 50 pg/µl
        NEBExpress® Competent E. coli (High Efficiency) C2523HVIAL -80 20 x 0.05 ml Not Applicable
    • C2523I     -80    
        SOC Outgrowth Medium B9020SVIAL 4 1 x 25 ml Not Applicable
        pUC19 Vector N3041AVIAL -20 1 x 0.025 ml 50 pg/µl
        NEBExpress® Competent E. coli (High Efficiency) C2523IVIAL -80 6 x 0.2 ml Not Applicable

  • 特性和用法

    Antibiotic for Plasmid Selection

    用于质粒筛选的抗生素 使用浓度
    Ampicillin 100 µg/ml
    Carbenicillin 100 µg/ml
    Chloramphenicol 33 µg/ml
    Kanamycin 30 µg/ml
    Tetracycline 15 µg/ml
    Streptomycin 25 µg/ml

    货运单

    • Ships on dry ice

    抗生素耐药性

    • nit

  • 优势和特性

    Features

    • Versatile non-T7 expression strain
    • Protease deficient

    应用特性

    Chemically competent E. coli cells suitable for high efficiency transformation and protein expression. Recommended host strain for pMAL Protein Fusion and Purification System.

     

    DNA Effects on Transformation Efficiency and Colony Output: Express® Competent E. coli (High Efficiency) |
    The optimal amount of DNA to use in a transformation reaction is lower than commonly recognized. Using clean, supercoiled pUC19, the efficiency of transformation is highest in the 100 pg-1 ng range. However, the total colonies which can be obtained from a single transformation reaction increase up to about 100 ng.
    Effect of heat shock time on NEBExpress competent E. coli transformation efficiency: Express® Competent E. coli (High Efficiency) |  
    50 μl of competent cells were transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol except heat shock time varied from 0 to 80 seconds.
    Effect of DNA incubation time on NEB Express competent E. coli transformation efficiency: Express® Competent E. coli (High Efficiency) |
    50 μl of competent cells were transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol except DNA incubation time varied from 0 to 40 minutes.

  • 相关产品

    相关产品

    • c3037-nebexpress-iq-competent-e-coli-high-efficiency

    单独销售的组分

    • SOC Outgrowth 培养基

  • 注意事项

    1. 注意：本产品含有害物质 DMSO。处理前请先查看 MSDS。
    2. 贮存和处理：感受态细胞应在 -80℃ 下贮存。在 -20℃ 贮存会导致转化效率显著降低。当细胞温度高于 -80℃ 时，即使不融化，细胞也会失去效率。

操作说明、说明书 & 用法

  • 操作说明

    1. High Efficiency Transformation (C2523)
    2. 5 Minute Transformation (C2523)
    3. Expression Using NEB Express (C2523)
    4. High Efficiency Transformation Protocol (NEB #C2523 and #C3037)

  • 使用指南

    • Additional E. coli Strain Genotypes
    • Competent E. coli Genome Sequences Tool
    • Genetic Markers
    • Making Unmethylated (Dam- Dcm-) DNA
    • McrA, McrBC and EcoKI Strain Phenotypes
    • Restriction of Foreign DNA by E. coli K-12

  • 应用实例

    • Using the PURExpress® In Vitro Protein Synthesis Kit for Heterologous In Vitro Expression and Functional Screening of FMN-dependent Oxidoreductase Variants
    • Enhancing Transformation Efficiency

工具 & 资源

  • 选择指南

    • Characteristics of Select E.coli Strains
    • Competent Cell Selection Guide

FAQs & 问题解决指南

  • FAQs

    1. Does New England Biolabs offer strains of Competent E.coli suited for protein expression?
    2. How long should I incubate cells on ice after DNA has been added (NEB #C2523H and NEB #C2523I)?
    3. What are the solutions/recipes (C2523)?
    4. What are the strain properties (C2523)?
    5. What is the difference between NEB #C2523H and NEB #C2523I?
    6. Why are there no colonies or no growth in liquid culture (C2523)?
    7. Why is induced protein insoluble (C2523)?
    8. Why is there no protein visible on gel or no activity (C2523)?
    9. Can I store competent cells at -20°C instead of -80°C?
    10. Can the NEBExpress Competent E.coli (High Efficiency) be used for the expression of constructs containing a T7 promoter?
    11. What is the optimal heat shock time for this strain (NEB #C2523H and NEB #C2523I)?
    12. Which kind of transformation tubes should be used?
    13. What volume of DNA can be added into competent cells?
    14. What is the shelf life for this strain (NEB #C2523H and NEB #C2323I)?
    15. Are NEB’s competent cells compatible with the “Mix & Go” protocol?
    16. How should I store SOC Outgrowth Medium? The SOC I received with my competent cells recommends storage at either room temperature or 4°C, however, when I purchase it as a stand alone product, it recommends storing it at 4°C. Which is better?