Express® Iq Competent E. coli (High Efficiency) |NEB酶试剂 New England Biolabs

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产品信息

重点

  • Enhanced BL21 derivative ideal for Plac, Ptac, Ptrc expression vectors
  • LacIq reduces basal expression
  • Ideal for pUC19 expression
  • Deficient in proteases Lon and OmpT
  • Resistant to phage T1 (fhuA2)
  • Does not restrict methylated DNA (McrA–, McrBC–, EcoBr–m–, Mrr–)
  • Better control of IPTG induced expression of non T7 plasmids
  • Free of animal products

基因型

MiniF lacIq (CamR) / fhuA2 [lon] ompT gal sulA11 R(mcr-73::miniTn10–TetS)2 [dcm] R(zgb-210::Tn10–TetS) endA1 Δ(mcrC-mrr)114::IS10

产品类别:
Non-T7 Expression,
E. coli Protein Expression Strains Products,
Protein Expression Products,

E. coli Expression Strains Products

应用:
Non-T7 Expression,
Protein Expression in E. Coli,
Protein Expression

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • C3037I     -80    
        NEBExpress® Iq Competent E. coli (High Efficiency) C3037IVIAL -80 6 x 0.2 ml Not Applicable

  • 特性和用法

    Antibiotic for Plasmid Selection

    用于质粒筛选的抗生素 使用浓度
    Ampicillin 100 µg/ml
    Carbenicillin 100 µg/ml
    Kanamycin 30 µg/ml
    Streptomycin 25 µg/ml
    Tetracycline 15 µg/ml

    货运单

    • Ships on dry ice

    抗生素耐药性

    • nit
    • cam

  • 优势和特性

    Features

    • Control of IPTG induced expression from Plac, Ptac,Ptrc and PT5-lacO
    • Protease deficient

    应用特性

    Chemically competent E. coli cells suitable for high efficiency transformation and protein expression, featuring control of IPTG induced expression with non-T7 plasmids.

    DNA Effects on Transformation Efficiency and Colony Output: Express® Iq Competent E. coli (High Efficiency) |
    The optimal amount of DNA to use in a transformation reaction is lower than commonly recognized. Using clean, supercoiled pUC19, the efficiency of transformation is highest in the 100 pg-1 ng range. However, the total colonies which can be obtained from a single transformation reaction increase up to about 100 ng.
    Effect of heat shock time on NEB Express Iq competent E. coli transformation efficiency: Express® Iq Competent E. coli (High Efficiency) |
    50 μl of competent cells were transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol except heat shock time varied from 0 to 80 seconds.
    Effect of DNA incubation time on NEB Express Iq competent E. coli transformation efficiency: Express® Iq Competent E. coli (High Efficiency) |
    50 μl of competent cells were transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol except DNA incubation time varied from 0 to 40 minutes.

  • 相关产品

    相关产品

    • c2523-neb-express-competent-e-coli-high-efficiency

    单独销售的组分

    • SOC Outgrowth 培养基

  • 注意事项

    1. 注意：本产品含有害物质 DMSO。处理前请先查看 MSDS。
    2. 贮存和处理：感受态细胞应在 -80℃ 下贮存。在 -20℃ 贮存会导致转化效率显著降低。当细胞温度高于 -80℃ 时，即使不融化，细胞也会失去效率。

操作说明、说明书 & 用法

  • 操作说明

    1. High Efficiency Transformation Protocol (C3037)
    2. 5 Minute Transformation Protocol (C3037)
    3. Protocol for Expression Using NEB Express Iq (C3037)
    4. High Efficiency Transformation Protocol (NEB #C2523 and #C3037)

  • 使用指南

    • Additional E. coli Strain Genotypes
    • Competent E. coli Genome Sequences Tool
    • Genetic Markers
    • McrA, McrBC and EcoKI Strain Phenotypes

  • 应用实例

    • AppNote_Enhancing_Transformation_Efficiency
    • Enhancing Transformation Efficiency

工具 & 资源

  • 选择指南

    • Characteristics of Select E.coli Strains
    • Competent Cell Selection Guide

FAQs & 问题解决指南

  • FAQs

    1. Does New England Biolabs offer strains of Competent E.coli suited for protein expression?
    2. How long should I incubate cells on ice after DNA has been added (NEB #C3037H and NEB #C3037I)?
    3. What are the solutions/recipes (C3037)?
    4. What are the strain properties (C3037)?
    5. What is the difference between NEB #C3037H and NEB #C3037I?
    6. What is the optimal heat shock time for this strain (NEB #C3037H and NEB #C3037I)?
    7. Why are there no colonies or no growth in liquid culture (C3037)?
    8. Why is induced protein insoluble (C3037)?
    9. Why is there no protein visible on gel or no activity (C3037)?
    10. Can I store competent cells at -20°C instead of -80°C?
    11. What is the shelf life for this strain (NEB #C3037H and NEB #C3037I)?
    12. Which kind of transformation tubes should be used?
    13. What volume of DNA can be added into competent cells?
    14. Are NEB’s competent cells compatible with the “Mix & Go” protocol?
    15. Is antibiotic selection necessary to maintain the MiniF-lacIq plasmid?