RNase Inhibitor, Human Placenta |NEB酶试剂 New England Biolabs

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产品信息

RNase Inhibitor, Human Placenta is a recombinant human placental protein which specifically inhibits ribonucleases (RNases) A, B and C (1). It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor, Human Placenta is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3).

The 50 kDa protein inhibits RNases by binding noncovalently in a 1:1 ratio with an association constant greater than 1014 (2).

产品来源

An E. coli strain that carries the Ribonuclease Inhibitor gene from human placenta.

产品类别:
RNase Inhibitors,
RNA Synthesis In vitro Transcription (IVT)

应用:
RT-PCR & cDNA Synthesis

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • M0307S     -20    
        RNase Inhibitor, Human Placenta M0307SVIAL -20 1 x 0.05 ml 40,000 units/ml
    • M0307L     -20    
        RNase Inhibitor, Human Placenta M0307LVIAL -20 1 x 0.25 ml 40,000 units/ml

  • 特性和用法

    单位定义

    One unit is defined as the amount of RNase Inhibitor, Human Placenta required to inhibit the activity of 5 ng of RNase A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2, 3′-cyclic monophosphate by RNase A.

    贮存溶液

    50 mM KCl
    8 mM DTT
    50% Glycerol
    20 mM HEPES-KOH
    pH 7.6 @ 25°C

  • 注意事项

    1. Since ribonucleases typically retain activity under denaturing conditions, care must be taken to avoid denaturing RNase Inhibitor molecules which have complexed with a ribonuclease. To prevent the release of active ribonuclease, temperatures greater than 50°C and high concentrations of urea or other denaturing agents should be avoided.
    2. The recommended concentration of RNase Inhibitor in a reaction is 1 unit/μl. During assembly of a reaction, RNase Inhibitor should be added before other components that are possible sources of RNase contamination (i.e. enzymes, plasmid from a mini prep.)

  • 参考文献

    1. Blackburn, P. and Moore, S. (1982). Pancreatic Ribonucleases . The Enzymes. XV, Part B, Academic Press, NY.
    2. Blackburn, P., Wilson, G. and Moore, S. (1977). J. Biol. Chem. 252,5904.

操作说明、说明书 & 用法

  • 操作说明

    1. Protocol for Avoiding Rnase Contamination using Rnase Inhibitor, Human Placenta (M0307)