上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
产品信息
RNase 4 is a single-stranded RNA endonuclease that cleaves 3´ of uridine in uridine-purine sequences (cut sites: U/A and U/G). RNase 4 allows more targeted digestion of substrate RNA compared to single-nucleotide specific RNases like T1 (cut site: after G), RNase U2 (cut sites: after A and G), or bovine pancreatic RNase A (cut sites: after C and U). RNase 4 endonuclease activity tolerates uridine base modifications such as pseudo-, N1-methyl-pseudo-, dihydro-, and 5-methoxy-uridine species (Ψ, m1Ψ, D, and mo5U). Due to the chemical mechanism of RNase 4 endonucleolytic cleavage, product oligonucleotides contain heterogenous 3´ ends, where most species contain a linear 3´-phosphate or cyclic 2´,3´-phosphate.
产品来源
An Escherichia coli strain that carries the cloned RNASE4 gene from Homo sapiens, with an N-terminal 6xHis tag.
产品类别:
RNases
产品组分信息
本产品提供以下试剂或组分:
NEB #
名称
组分货号
储存温度
数量
浓度
M1284S
-20
RNase 4
M1284SVIAL
-20
1 x 0.05 ml
50,000 units/ml
NEBuffer™ r1.1
B6001SVIAL
-20
1 x 1.25 ml
10 X
M1284L
-20
RNase 4
M1284LVIAL
-20
1 x 0.25 ml
50,000 units/ml
NEBuffer™ r1.1
B6001SVIAL
-20
1 x 1.25 ml
10 X
特性和用法
反应条件
1X NEBuffer™ r1.1 Incubate at 37°C
1X NEBuffer™ r1.1 10 mM Bis-Tris-Propane-HCl 10 mM MgCl2 100 µg/ml 重组白蛋白 (pH 7 @ 25°C)
贮存溶液
100 mM NaCl 50 mM NaOAc 200 µg/ml Recombinant Albumin 50% Glycerol pH 6 @ 25°C
热失活
否
参考文献
Wolf et al. (2022). Human RNase 4 improves mRNA sequence characterization by LC-MS/MS. NAR. PubMedID: 35871301
Wolf et al. (2023). Selective Characterization of mRNA 5′ End-Capping by DNA Probe-Directed Enrichment with Site-Specific Endoribonucleases. ACS Pharmacol. Transl. Sci. PubMedID: 37974627
操作说明、说明书 & 用法
操作说明
RNase 4 (NEB #M1284) DNA Probe-Directed Analysis of mRNA 5´Cap Structures
RNase 4 (NEB #M1284) Digestion Protocol
FAQs & 问题解决指南
FAQs
Does RNase 4 (NEB #M1284) cut RNA at sites other than U/A and U/G?
How much RNase 4 (NEB #M1284) should I use to digest RNA for LC-MS/MS nucleotide sequence mapping?
What is the chemical identity of 5´ and 3´ oligonucleotides generated after RNase 4 (NEB #M1284) cleavage?
How much RNase 4 (NEB #M1284) should I use for DNA-probe directed 5´-cap mRNA analysis?
Can RNase 4 (NEB #M1284) be inactivated?
Is RNase 4 (NEB #M1284) endonucleolytic activity sensitive to RNA modifications?
Does RNA secondary structure impact RNase 4 (NEB #M1284) activity?
Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?