Monarch RBC© 裂解液 |NEB酶试剂 New England Biolabs

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产品信息

Monarch RBC 裂解液专为裂解红细胞而设计，同时保持白细胞完好无损。单独的红细胞裂解步骤能够去除血红蛋白，提高从血液中提取 DNA 的纯度。RBC 裂解液还能去除核酸酶，以确保纯化的高分子量 DNA 大小达到 Mb 级。

产品类别:
Genomic DNA Extraction & Purification Products,
Nucleic Acid Purification Products

  • 试剂盒组成

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • T3051L     25    
        Monarch® RBC Lysis Buffer T3051-1 25 1 x 160 ml Not Applicable

  • 相关产品

    相关产品

    • Monarch 高分子量 DNA 提取试剂盒（细胞和血液）
    • T3060 Monarch HMW DNA Extraction Kit for Tissue
    • Monarch 2 ml 离心管
    • Monarch DNA 捕获珠
    • Monarch 捕获珠分离管
    • T3054 Monarch gDNA Nuclei Prep and Lysis Buffer Pack
    • T3056 Monarch gDNA Elution Buffer II
    • T3062 Monarch Protein Separation Solution
    • T3055 Monarch Precipitation Enhancer
    • Monarch® 收集管 II
    • Monarch 2 ml 离心管
    • T3015 Monarch gDNA Wash Buffer

操作说明、说明书 & 用法

  • 操作说明

    1. Download Quick Protocol Card for Blood
    2. Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Blood (NEB #T3050)
    3. Protocol for UHMW DNA Cleanup in the Oxford Nanopore Technologies® UL Library Prep Workflow
    4. Protocol for Isolation of Genomic DNA (gDNA) from up to 500 µl mammalian blood using the Monarch® Genomic DNA Purification Kit (NEB #T3010) and RBC Lysis Buffer (NEB #T3051)

  • 应用实例

    • Monarch® HMW DNA Extraction Kit improves sample preparation for Oxford Nanopore Technologies® sequencing of malaria parasites

FAQs & 问题解决指南

  • FAQs

    1. Will extended binding increase the yield of the target protein in the eluate?
    2. Can the Monarch HMW DNA Extraction Kits be used to process human Buffy coat?
    3. My blood sample is frozen in aliquots larger than is recommended in your protocol (e.g., in Vacutainers). Can I still use the frozen blood protocol for the Monarch HMW DNA Extraction Kit for Cells & Blood (NEB #T3050)?

  • 问题解决指南

    • Troubleshooting Guide for Monarch HMW DNA Extraction Kits (NEB #T3050 and #T3060)