Next® Poly(A) mRNA Magnetic Isolation Module |NEB酶试剂 New England Biolabs

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产品信息

Next® Poly(A) mRNA Magnetic Isolation Module |

The NEBNext Poly(A) mRNA Magnetic Isolation Module is designed to isolate intact poly(A)+ RNA from 1-5µg of previously isolated total RNA. The technology is based on the coupling of Oligo d(T)25 to 1 μm paramagnetic beads which is then used as the solid support for the direct binding of poly(A)+ RNA. Thus, the procedure permits the manual processing of multiple samples and can be adapted for automated high-throughput applications. Additionally, magnetic separation technology permits elution of intact mRNA in small volumes eliminating the need for precipitating the poly(A)+ transcripts in the eluent. Intact poly(A)+ RNA which is fully representative of the mRNA population of the original sample can be obtained in less than one hour.

Note that for input amounts >5µg we recommend the NEBNext High Input Poly(A) mRNA Isolation Module (NEB #E3370).

产品类别:
RNA Depletion & mRNA Enrichment Products,
RNA Library Prep for Illumina,
Next Generation Sequencing Library Preparation Products

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • E7490S     4    
        NEBNext RNA Binding Buffer (2X) E7492AVIAL 4 1 x 7.2 ml Not Applicable
        NEBNext Wash Buffer E7493AVIAL 4 1 x 28.8 ml Not Applicable
        Nuclease-free Water E7495AVIAL 4 1 x 1.2 ml Not Applicable
        NEBNext Tris Buffer E7496AVIAL 4 1 x 6 ml Not Applicable
        NEBNext Oligo d(T)25 Beads E7499AVIAL 4 1 x 0.48 ml Not Applicable
    • E7490L     4    
        NEBNext RNA Binding Buffer (2X) E7492AAVIAL 4 1 x 28.8 ml Not Applicable
        NEBNext Wash Buffer E7493AAVIAL 4 2 x 57.6 ml Not Applicable
        Nuclease-free Water E7495AAVIAL 4 1 x 4.8 ml Not Applicable
        NEBNext Tris Buffer E7496AAVIAL 4 1 x 24 ml Not Applicable
        NEBNext Oligo d(T)25 Beads E7499AAVIAL 4 1 x 1.92 ml Not Applicable

  • 特性和用法

    需要但不提供的材料

    • 96-well 0.2 ml PCR Plates and Microseal® 'B' Adhesive Sealer (BioRad MSB-1001) or 0.2 ml RNase-free tube.
    • Magnetic rack or plate (e.g., NEBNext® Magnetic Separation Rack (NEB #S1515S), Alpaqua ® 96S Super Magnet Plate (#A001322), or equivalent)
    • Thermal cycler or heat block
    • Bioanalyzer® (Agilent Technologies, Inc.) or similar instrument and consumables.

  • 优势和特性

    应用特性

    Isolation of poly(A)+ RNA transcript from Total RNA for RNA library preparation and sequencing.

操作说明、说明书 & 用法

  • 操作说明

    1. Where can I find protocols for using NEBNext® RNA Library Prep reagents?

  • 说明书

    产品说明书包含产品使用的详细信息、产品配方和质控分析。

    • manualE7490

  • 使用指南

    • Getting Started with RNA-Sequencing (RNA-Seq)

FAQs & 问题解决指南

  • FAQs

    1. Can I isolate poly(A)+ mRNA directly from tissue lysis using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (E7490)?
    2. Do I need two rounds of binding?
    3. Can I use the Oligo (dT)25 Magnetic Beads (NEB# S1419S) to isolate poly(A)+ mRNA for Next Generation Sequence applications?