上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
产品信息
NEBNext 接头采用了一种新型的发卡环状结构,能以更高的效率连接末端经修复和加 dA 尾的 DNA 片段。环状结构 DNA 中包含一个 U,当 U 被 USER® 酶(UDG 和 Endo VIII 的混合物)切掉后,环状结构打开,使它可以成为 PCR 反应的底物。通过 NEBNext Index 引物在 PCR 步骤中引入 Barcode,从而有助于实现多重测序文库制备。该试剂盒包含 8 种 i5 Index 引物和 12 种 i7 Index 引物。
NEBNext 接头引物可与 NEBNext 产品以及其它兼容 Illumina 平台的标准文库制备流程结合使用。
还提供双端 Index 引物(NEB #E7600)。还提供 12 种和 96 种单端 Index 引物(NEB #E7335、 NEB #E7500、NEB #E7710、NEB #E7730、NEB #E6609)。也提供甲基化的 NEBNext 接头(NEB #E7535),可用于重亚硫酸盐测序流程。
- 产品类别:
- NEBNext® Multiplex Oligos (Adaptors & Primers) Products
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试剂盒组成
本产品提供以下试剂或组分:
NEB # 名称 组分货号 储存温度 数量 浓度 -
E7780S -20 NEBNext Adaptor for Illumina E7601AVIAL -20 1 x 0.96 ml Not Applicable USER® Enzyme E7602AVIAL -20 1 x 0.288 ml Not Applicable NEBNext® i509 Primer E7781AVIAL -20 1 x 0.06 ml 10 µM NEBNext® i510 Primer E7782AVIAL -20 1 x 0.06 ml 10 µM NEBNext® i511 Primer E7783AVIAL -20 1 x 0.06 ml 10 µM NEBNext® i512 Primer E7784AVIAL -20 1 x 0.06 ml 10 µM NEBNext® i513 Primer E7785AVIAL -20 1 x 0.06 ml 10 µM NEBNext® i514 Primer E7786AVIAL -20 1 x 0.06 ml 10 µM NEBNext® i515 Primer E7787AVIAL -20 1 x 0.06 ml 10 µM NEBNext® i516 Primer E7788AVIAL -20 1 x 0.06 ml 10 µM NEBNext® i713 Primer E7789AVIAL -20 1 x 0.04 ml 10 µM NEBNext® i714 Primer E7790AVIAL -20 1 x 0.04 ml 10 µM NEBNext® i715 Primer E7791AVIAL -20 1 x 0.04 ml 10 µM NEBNext® i716 Primer E7792AVIAL -20 1 x 0.04 ml 10 µM NEBNext® i717 Primer E7793AVIAL -20 1 x 0.04 ml 10 µM NEBNext® i718 Primer E7794AVIAL -20 1 x 0.04 ml 10 µM NEBNext® i719 Primer E7795AVIAL -20 1 x 0.04 ml 10 µM NEBNext® i720 Primer E7796AVIAL -20 1 x 0.04 ml 10 µM NEBNext® i721 Primer E7797AVIAL -20 1 x 0.04 ml 10 µM NEBNext® i722 Primer E7798AVIAL -20 1 x 0.04 ml 10 µM NEBNext® i723 Primer E7799AVIAL -20 1 x 0.04 ml 10 µM NEBNext® i724 Primer E7800AVIAL -20 1 x 0.04 ml 10 µM
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相关产品
相关产品
- e7760-nebnext-ultra-ii-directional-rna-library-prep-kit-for-illumina
- e7765-nebnext-ultra-ii-directional-rna-library-prep-with-sample-purification-beads
- NEBNext® Ultra™ II RNA 非链特异性文库制备试剂盒
- e7775-nebnext-ultra-ii-rna-library-prep-with-sample-purification-beads
- NEBNext® Ultra™ II FS DNA 文库制备试剂盒 – 含片段化酶
- NEBNext® Ultra™ II FS DNA 文库制备试剂盒 – 含片段化酶(含纯化磁珠)
- NEBNext® Ultra™ II DNA 文库制备试剂盒
- NEBNext® Ultra™ II DNA 文库制备试剂盒(含纯化磁珠)
- NEBNext® Ultra™ II 连接模块
- NEBNext® Ultra™ DNA 文库制备试剂盒
- NEBNext® Ultra 连接模块
- NEBNext® 快速连接模块
操作说明、说明书 & 用法
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操作说明
- Setting up the PCR Reaction – NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 2) (E7780)
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说明书
产品说明书包含产品使用的详细信息、产品配方和质控分析。- manualE7780
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使用指南
- E7780_DualIndex_IlluminaLRM
- Forward Strand Workflow Sample Sheet for E7780: NovaSeq 6000 with v1.0 reagent kits, MiniSeq with Rapid reagent kits, MiSeq, HiSeq 2000/2500 (paired-end flow cell), HiSeq 3000/4000 (single-read flow cell)
- Reverse Complement Workflow Sample Sheet for E7780: iSeq 100, MiniSeq with Standard reagent kits, NextSeq Systems, NovaSeq 6000 with v1.5 reagent kits, HiSeq 2000/2500 (single-read flow cell), HiSeq 3000/4000 (paired-end flow cell)
工具 & 资源
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选择指南
- NEBNext® Multiplex Oligos Selection Chart
FAQs & 问题解决指南
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FAQs
- Where can I find the sample sheets for these Multiplex Oligos?
- Are NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 2) supplied with library prep reagents?
- Can I use NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 2) for library preparation from DNA and RNA samples?
- How many libraries can I prepare with the NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 2)?
- For pooling <96 samples, what combination of indices should I use?
- Do I need to spike in custom sequencing primers when sequencing libraries are made with NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 2) on Illumina sequencing instruments?
- Can I perform single read runs and still get both index sequences? Can I run both single read and paired-end recipes with dual-indexed libraries?
- Is the USER® Enzyme in the NEBNext Oligos kits identical in concentration to the standalone product?
- How do I use the NEB provided Sample Sheet for my specific instrument?
- Are dual indexed libraries compatible with single end sequencing?
- What sequences need to be trimmed for NEBNext libraries that are sequenced on an Illumina instrument?