上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
产品信息
Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5).
It is supplied with 10X ThermoPol Reaction Buffer, which contains a nonionic detergent to increase enzyme stability during longer incubations.
重点
- Isolated from a recombinant source
- Supplied with 10X Reaction Buffer
- Robust and reliable reactions
- Tolerates a wide range of templates
- Incorporates dUTP, dITP and fluorescently-labeled nucleotides
- Exceptional value in terms of cost per unit
产品来源
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1
- 产品类别:
- Taq DNA Polymerase Products
- 应用:
- Colony PCR,
- A-tailing,
- Polymerases for DNA Manipulation,
- Multiplex PCR,
- Specialty PCR,
- Routine PCR,
PCR
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产品组分信息
本产品提供以下试剂或组分:
NEB # 名称 组分货号 储存温度 数量 浓度 -
M0267V -20 Taq DNA Polymerase with ThermoPol® Buffer M0267VVIAL -20 1 x 0.04 ml 5,000 units/ml ThermoPol® Reaction Buffer B9004SVIAL -20 1 x 1.5 ml 10 X
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M0267S -20 Taq DNA Polymerase with ThermoPol® Buffer M0267SVIAL -20 1 x 0.08 ml 5,000 units/ml ThermoPol® Reaction Buffer B9004SVIAL -20 2 x 1.5 ml 10 X
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M0267L -20 Taq DNA Polymerase with ThermoPol® Buffer M0267LVIAL -20 1 x 0.4 ml 5,000 units/ml ThermoPol® Reaction Buffer B9004SVIAL -20 4 x 1.5 ml 10 X
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M0267X -20 Taq DNA Polymerase with ThermoPol® Buffer M0267XVIAL -20 1 x 0.8 ml 5,000 units/ml ThermoPol® Reaction Buffer B9004SVIAL -20 8 x 1.5 ml 10 X
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M0267E -20 Taq DNA Polymerase with ThermoPol® Buffer M0267XVIAL -20 5 x 0.8 ml 5,000 units/ml ThermoPol® Reaction Buffer B9004SVIAL -20 40 x 1.5 ml 10 X
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特性和用法
单位定义
One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.
反应条件
1X ThermoPol® Reaction Buffer
1X ThermoPol® Reaction Buffer
20 mM Tris-HCl
10 mM (NH4)2SO4
10 mM KCl
2 mM MgSO4
0.1% Triton® X-100
(pH 8.8 @ 25°C)贮存溶液
10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
0.5% Tween® 20
0.5% IGEPAL® CA-630
50% Glycerol
pH 7.4 @ 25°C热失活
否
分子量
理论上的: 94000 daltons
5′ – 3′ 核酸外切酶
Yes
3′ – 5′ 核酸外切酶
No
链置换
+
产物末端
- 3´ 单碱基突出末端
单位活性检测条件
1X ThermoPol Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.
错配率
~ 285×10-6bases
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优势和特性
应用特性
- PCR
- Primer extension
- Colony PCR
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相关产品
相关产品
- Taq 5X 预混液
- Taq 2X 预混液
- Taq PCR 试剂盒
- dNTP 混合液
- dNTP 套装
- Quick-Load® Taq 2X 预混液
- Magnesium Sulfate (MgSO4) Solution
单独销售的组分
- ThermoPol® 反应缓冲液套装
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注意事项
- 5´→3´ 结构特异性核酸内切酶活性会降解置换链。
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参考文献
- Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact.. 127, 1550-1557.
- Kaledin, A.S., Sliusarenko, A.G. and Gorodetskii, S.I. (1980). Biokhimiya. 45, 644-651.
- Lawyer, F.C. et al. (1993). PCRMethods and Appl.. 2, 275-287.
- Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990). NucleicAcids Res.. 18, 7317-7322.
- Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.
- Saiki R.K. et al. (1985). Science. 230, 1350-1354.
- Powell, L.M. et al. (1987). Cell. 50, 831-840.
- Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
- Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). Nucleic Acids Res.. 18, 7465.
操作说明、说明书 & 用法
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操作说明
- PCR Protocol for Taq DNA Polymerase with ThermoPol® Buffer (M0267)
- A-Tailing with Taq Polymerase
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使用指南
- Activity of Restriction Enzymes in PCR Buffers
- Guidelines for PCR Optimization with Taq DNA Polymerase
- Guidelines for PCR Optimization with Thermophilic DNA Polymerases
工具 & 资源
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选择指南
- DNA Polymerase Selection Chart
- NEB Diluent and Buffer Table
- Taq Buffer Selection Chart
- Thermophilic DNA Polymerases
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Web 工具
- Tm Calculator
FAQs & 问题解决指南
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FAQs
- What ends will my PCR products have?
- How should I determine the appropriate annealing temperature for my reaction?
- What should the final primer concentration be in my PCR?
- What are the properties of this polymerase (fidelity, product ends, max amplicon, modified base incorporation, etc.)?
- What are the stability and storage requirements for NEB’s Taq and OneTaq DNA Polymerases?
- Is this Taq DNA Polymerase product compatible with other NEB Buffers?
- Can Taq/OneTaq® DNA Polymerases be used for nick translation?
- How can I optimize my PCR when using Taq DNA Polymerase?
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问题解决指南
- PCR Troubleshooting Guide
- Taq PCR Kit Troubleshooting Guide
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实验技巧
Did you know most Taq reactions amplify more efficiently and robustly when you use a 68°C extension temperature?