Bst 2.0 WarmStart DNA Polymerase (Glycerol-free) |NEB酶试剂 New England Biolabs

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产品信息

Bst 2.0 WarmStart DNA Polymerase (Glycerol-free) is formulated without glycerol to support lyophilization, incorporation into microfluidic devices, and enable quick adoption into automation workflows.

Bst 2.0 WarmStart DNA Polymerase is an in silico designed homolog of Bacillus stearothermophilus DNA Polymerase I, Large Fragment (Bst DNA Polymerase, Large Fragment) with a reversibly-bound aptamer, which inhibits polymerase activity at temperatures below 45°C. At temperatures above 45°C, the aptamer rapidly releases from the enzyme with no special activation step needed to activate the polymerase. Bst 2.0 WarmStart DNA Polymerase contains 5´→3´ DNA polymerase activity and strong strand-displacement activity but lacks 5´→3´ exonuclease activity. Bst 2.0 WarmStart DNA Polymerase displays improved amplification speed, yield, salt tolerance, and thermostability compared to wild-type Bst DNA Polymerase, Large Fragment.

Bst 2.0 WarmStart DNA Polymerase (Glycerol-free) offers the same robust detection of human DNA targets in LAMP assays as the glycerol-containing enzyme.

Bst 2.0 WarmStart DNA Polymerase (Glycerol-free) |

LAMP (DNA targets) experiments were performed with NEB #M0538: Bst 2.0 WarmStart DNA Polymerase or NEB #M0402: Bst 2.0 WarmStart DNA Polymerase (Glycerol-free). Reactions containing 1X LAMP primers and 0.5X LAMP Fluorescent dye were set up in quadruplicate over three logs of total Jurkat DNA (10 ng to 0.1 ng) in 96-well, 25 µl reactions. Control reactions without template (NTC) were also evaluated. Reactions were incubated at 65°C for 40 minutes and fluorescence was monitored every 15 seconds in the SYBR/FAM channel of a real-time thermocycler (Bio-Rad® CFX96). Each dot represents the time at which the fluorescence signal for a single reaction crosses the instrument-defined threshold. All four replicates were detected at each template input unless otherwise indicated (note that dots frequently overlap given similar detection time for the replicates). Overall, similar performance was observed for both glycerol-containing (NEB #M0538) and glycerol-free (NEB #M0402) enzymes at each template input. No amplification was observed in any of the no template control reactions.

产品类别:
Isothermal Amplification & Strand Displacement Products

应用:
Isothermal Amplification,
DNA Amplification, PCR & qPCR

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • M0402L     -80    
        Bst 2.0 WarmStart DNA Polymerase (Glycerol-free) M0402LVIAL -80 1 x 0.067 ml 120,000 units/ml
        10X Isothermal Amplification Buffer (Lyo-compatible) B1714SVIAL -20 2 x 1.25 ml 10 X

操作说明、说明书 & 用法

  • 操作说明

    1. Typical LAMP Protocol (NEB #M0402)

工具 & 资源

  • Web 工具

    • NEB LAMP Primer Design Tool

FAQs & 问题解决指南

  • FAQs

    1. What is the difference between Bst DNA Polymerase, Large Fragment and Bst 2.0 DNA Polymerase?
    2. Why would I use Bst 2.0 WarmStart® DNA Polymerase?
    3. Can Bst 2.0 DNA Polymerase be heat inactivated?
    4. Are NEB DNA Polymerases supplied with dNTPs?
    5. Can Bst 2.0 DNA Polymerase be used in applications requiring thermal cycling?
    6. Can Bst 2.0 DNA Polymerase initiate at a nick in the DNA?
    7. When should Bst 2.0 DNA Polymerase be the enzyme of choice?
    8. Can Bst 2.0 DNA Polymerase be used at temperatures other than 65°C?
    9. Does Bst 2.0 DNA polymerase incorporate dUTP?
    10. How do I use Antarctic Thermolabile UDG for carryover prevention in LAMP reactions?
    11. What is LAMP and RT-LAMP?
    12. What are Hot Start and WarmStart® polymerases and when would I use them?
    13. What is the difference between buffers B1714: 10X Isothermal Amplification Buffer (Lyo-compatible) and B0537: 10X Isothermal Amplification Buffer?
    14. What is the Mg2+ concentration in the 10X Isothermal Amplification Buffer (Lyo-compatible)?
    15. Does B1714: 10X Isothermal Amplification Buffer (Lyo-compatible) include any excipients?
    16. Can Bst 2.0 WarmStart® DNA Polymerase (Glycerol-free) at 120,000 U/ml be diluted prior to use?
    17. How many Freeze/Thaw cycles can Bst 2.0 WarmStart® DNA Polymerase (Glycerol-free) tolerate?

质控、安全 & 法规

  • 质控分析

    每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。

  • 产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

    • M0402L_v1

  • CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

    • M0402L_v1_10225700

  • SDS

    以下 SDS 文件可以帮助您安全地使用该产品

    • Bst 2.0 WarmStart DNA Polymerase (Glycerol-free)

  • 法律 & 免责声明

    产品及内容受 New England Biolabs, Inc（NEB）所拥有或控制的一项或多项专利、商标和/或版权保护。文中使用商标符号并不一定表明其在出现的国家/地区注册为商标，仅表明已在创始地注册。如将本产品用于特定应用，用户可能需要获取第三方知识产权授权许可。了解更多信息，请邮件咨询 [email protected]。

    本产品仅用于科研。本产品不适用于人类或动物的治疗或诊断。

    New England Biolabs（NEB）致力于开展合乎伦理的科学研究 – 我们坚信，作为科研工作者，我们的工作是提出重要问题，解决这些问题，将有助于维护我们的生活质量和我们所生活的世界。然而，研究必须始终以安全且合乎道德的方式进行。了解更多。