Bst DNA Polymerase, Full Length |NEB酶试剂 New England Biolabs

发表于由

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

产品信息

Bst DNA Polymerase, Full Length is the full length polymerase from Bacillus stearothermophilus. It has 5´ → 3´ polymerase and double-strand specific 5´ → 3´ exonuclease activity, but lacks 3´ → 5´ exonuclease activity (1).

产品来源

An E. coli strain that contains the gene from Bacillus stearothermophilus. (H. Kong)

产品类别:
Isothermal Amplification & Strand Displacement Products

应用:
Isothermal Amplification

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • M0328S     -20    
        Bst DNA Polymerase, Full Length M0328SVIAL -20 1 x 0.1 ml 5,000 units/ml
        ThermoPol® Reaction Buffer B9004SVIAL -20 1 x 1.5 ml 10 X

  • 特性和用法

    单位定义

    One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C.

    反应条件

    1X ThermoPol® Reaction Buffer
    Incubate at 65°C

    1X ThermoPol® Reaction Buffer
    20 mM Tris-HCl
    10 mM (NH4)2SO4
    10 mM KCl
    2 mM MgSO4
    0.1% Triton® X-100
    (pH 8.8 @ 25°C)

    贮存溶液

    10 mM Tris-HCl
    50 mM KCl
    1 mM DTT
    0.1 mM EDTA
    50% Glycerol
    0.1% Triton® X-100
    pH 7.1 @ 25°C

    热失活

    80°C for 20 minutes

    5′ – 3′ 核酸外切酶

    Yes

    3′ – 5′ 核酸外切酶

    No

    链置换

    No

    单位活性检测条件

    50 mM KCl, 20 mM Tris-HCl (pH 8.8), 10 mM MgCl2, 30 nM M13mp18 SS DNA, 70 nM M13 sequencing primer (–47) 24-mer, 200 µM dATP, 200 µM dCTP, 200 µM dGTP, 100 µM dTTP including [3H]-dTTP and 100 µg/ml BSA.

  • 相关产品

    相关产品

    • dNTP 套装
    • Magnesium Sulfate (MgSO4) Solution
    • dNTP 混合液

    单独销售的组分

    • ThermoPol® 反应缓冲液套装

  • 注意事项

    1. Bst DNA Polymerase does not exhibit 3´→ 5´ exonuclease activity.
    2. Reaction temperatures above 70°C are not recommended.
    3. Cannot be used for thermal cycle sequencing or PCR.

  • 参考文献

    1. Aliotta, J.M. et al. (1996). Genetic Analysis. 12, 185-195.
    2. Mead, D.A. et al. (1991). BioTechniques. 11, 76-87.

工具 & 资源

  • 选择指南

    • DNA Polymerase Selection Chart

FAQs & 问题解决指南

  • FAQs

    1. What are Hot Start and WarmStart® polymerases and when would I use them?

  • 问题解决指南

    • PCR Troubleshooting Guide