Next® Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1) |NEB酶试剂 New England Biolabs

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产品信息

Next® Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1) |

Incorporating a novel hairpin loop structure, the NEBNext adaptor ligates with increased efficiency to end-repaired, dA-tailed DNA. The loop contains a U, which is removed by treatment with USER® Enzyme (a mix of UDG and Endo VIII), to open up the loop and make it available as a substrate for PCR. During PCR, barcodes can be incorporated by use of the NEBNext index primers, thereby enabling multiplexing.

NEBNext oligos can be used with NEBNext products, and with other standard Illumina-compatible library preparation protocols.

产品类别:
Discontinued (<3 years)

  • 特性和用法

    需要但不提供的材料

    Compatible with the following library preparation kits:
    NEBNext Ultra™ II DNA Library Prep Kit for Illumina (#E7645),
    NEBNext Ultra™ DNA Library Prep Kit for Illumina (#E7370),
    NEBNext DNA Library Prep Master Mix Set for Illumina (#E6040), or
    NEBNext DNA Library Prep Reagent Set for Illumina (#E6000)

    NEB EpiMark® Bisulfite Conversion Kit (NEB # E3318S)
    NEB EpiMark Hot Start Taq DNA Polymerase (NEB # M0490S)

    10 mM dNTPs
    Freshly prepared 80% Ethanol
    Magnetic rack or plate (e.g., NEBNext® Magnetic Separation Rack (NEB #S1515S), Alpaqua ® 96S Super Magnet Plate (#A001322), or equivalent)
    DNA LoBind® Tubes (Eppendorf®)/PCR Plates
    Agencourt® AMPure® XP Beads (Beckman Coulter, Inc. #A63881)
    Bioanalyzer® (Agilent® Technologies, Inc.)
    Nuclease Free Water
    0.1X TE (or 10 mM Tris-HCl, pH 7.5-8.0)

操作说明、说明书 & 用法

  • 操作说明

    1. Please see manual (NEB #E7535) for protocols

  • 说明书

    产品说明书包含产品使用的详细信息、产品配方和质控分析。

    • manualE7535

  • 使用指南

    • E7535 NebNext Multiple Oligos for Illumina Sample Sheet

FAQs & 问题解决指南

  • FAQs

    1. What are the major applications of NEBNext Methylated Adaptor for Illumina?
    2. Is library prep workflow different when NEBNext Methylated Adaptor is used?
    3. How is NEBNext Methylated Adaptor (NEB #E7535) different from NEBNext adaptor for Illumina (NEB #E7335 & #E7350)?
    4. How do I know the concentration of my index primers? 
    5. What should I do if the sample sheet from the NEB.com website is not compatible with the software version I am using?