The Phytic Acid (Total Phosphorus) test kit is a simple method for the measurement and analysis of phytic acid/total phosphorus in food and feed samples. This method does not require purification of phytic acid via anion-exchange chromatography making it amenable to high numbers of samples.

Colourimetric method for the determination of Phytic Acid
in cereal products, seed materials, animal feeds and
other materials

Principle:
(phytase)
(1) Phytic acid + H₂O → myo-Inositol (phosphate)_n + P _i

(alkaline phosphatase)
(2) myo-Inositol (phosphate)_n + H₂O → myo-inositol + P_i

(3) P_i + ammonium molybdate → 12-molybdophosphoric acid

(diaphorase)
(4) 12-molybdophosphoric acid + H₂SO₄ / ascorbic acid →
molybdenum blue

Kit size:                            50 assays
Method:                            Spectrophotometric at 655 nm
Reaction time:                   25 min enzymic; 1 h for phosphate determination
Detection limit:                  ~ 11.3 mg phosphorus (~ 40 mg phytic acid) 
                                          /100 g material
Application examples: 
Seed materials, feeds and foodstuffs
Method recognition: Novel method

Advantages

• Very cost effective
  
• All reagents stable for > 2 years after preparation
  
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  
• Standard included