StemAb™ 干细胞用碱性磷酸酶染色试剂盒

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StemAb™ 干细胞用碱性磷酸酶染色试剂盒

StemAb™ 干细胞用碱性磷酸酶染色试剂盒



StemAb™ 干细胞用碱性磷酸酶染色试剂盒


碱性磷酸酶(AP)在多能干细胞中高水平表达。 AP 在碱性条件下使核苷酸、蛋白和生物碱去磷酸化。 染色后,未分化细胞呈现红色或紫色,而分化细胞呈现无色。

胚胎干细胞(ES)和诱导多能干细胞(iPS)的未分化状态可以通过高水平的碱性磷酸酶(AP)表达来表征,连同其他表面标志物的表达,表明未分化细胞具有自我更新潜力。AP 是一种水解酶,负责在碱性条件下使核苷酸,蛋白和生物碱去磷酸化。 当使用 AP 染色试剂盒对固定的 ES 或 iPS 细胞染色时,未分化细胞呈现红色或紫色,而分化细胞呈现无色。



◆试剂盒组成


固定溶液:  25 mL 

AP 染色溶液 A:10 mL
AP 染色溶液 B:10 mL

AP 染色溶液 C:10 mL



储存和稳定性


● 在 4°C 储存, 不可冷冻储存。到货后妥善存储可稳定 6 个月。



质量控制


StemAb™ 干细胞用碱性磷酸酶染色试剂盒已在人 iPS 细胞和小鼠 ES 细胞上进行功能测试,确保产品质量。

◆操作步骤


StemAb Alkaline Phosphatase Staining Kit II


※ 本页面产品仅供研究用,研究以外不可使用。

参考文献


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Smith, A.G., Nichols, J., Robertson, M., and Rathjen, P.D. (1992) Differentiation inhibiting activity (DIA/LIF) and mouse development. Dev. Biol. 151: 339-351. 全文

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Takahashi, K., Tanabe K., Ohnuki, M., Narita, M., Ichisaka T., Tomoda K. and Yamanaka, S. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131: 861-872. 全文


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Yu, J., Vodyanik, M.A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J.L., Tian, S., Nie, J., Jonsdottir, G.A., Ruotti, V., Stewart, R., Slukvin, I.I., and Thomson, J.A. (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science 318: 1917-1920.全文

◆其他相关资料

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产品编号 产品名称 产品规格 产品等级
00-0055 Stemgent Alkaline Phosphatase Staining Kit II (50 assays) 
Stemgent碱性磷酸酶染色试剂盒II
50 assays